| ProGlyProt ID | BC107 |
| Organism Information |
| Organism Name | Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) |
| Domain | Bacteria |
| Classification | Family: Bacteroidaceae
Order: "Bacteroidales"
Class: "Bacteroidia"
Division or phylum: "Bacteroidetes" |
| Taxonomic ID (NCBI) | 272559 |
| Genome Sequence (s) |
| GeneBank | CR626927.1 |
| EMBL | CR626927 |
| Gene Information |
| Gene Name | BF0810Â |
| NCBI Gene ID | 3288800 |
| GenBank Gene Sequence | 3288800 |
| Protein Information |
| Protein Name | BF0810 |
| UniProtKB/SwissProt ID | Q5LH32 |
| NCBI RefSeq | YP_210506.1 |
| EMBL-CDS | CAH06554.1 |
| UniProtKB Sequence | >tr|Q5LH32|Q5LH32_BACFN Putative alpha-L-fucosidase OS=Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) GN=BF0810 PE=4 SV=1
MKNNRLIITLIALFLLGFGLKAQTASTEETAAQKEKRMEWFAQAKLGIFIHWGIYAVNGV
SESWSFFNNYLPYEEYMAQEKGFTASAYNPQEWVKLIKESGARYTVITTKHHDGVALWDT
KAGDLSTVKSTPAGRDLIAPFVKEVRKQGLKLGFYYSLLDWSHPDYPNKTRTEVRYKNDP
DRWAKFVKFNFGQLSELNKTWKPDLYWFDGDWEQTAEAWDSKGIINLLRSTNPNVIVNSR
IQGYGDYATPEQGVPVVRPADKYWELCMTMNDSWGYQHADTNYKTPFMLLRTFVDCLSMG
GNLLLDIGPKEDGTIPAEQIAVLKEFGRWTKKHKEAIYETRAGIPCEHFQGYTTLNKAGD
ILYLYLPYKPNGPIEVKGLVNKVNRVWVVGNGAMLPYKVYNKNYWSEVPGNLYIDIPERV
QDEQITVIAVLLDGPIKLYRGVGQVIESN |
| Sequence length | 449 AA |
| Subcellular Location | |
| Function | Putative alpha-L-fucosidase. |
| Protein Structure |
| PDB ID | |
| Glycosylation Status |
| Glycosylation Type | O (Thr) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | T84, T281 (Engineered glycosylation site DTA was created at two positions F83D and N282A) |
| Experimentally Validated Glycosite(s ) in Mature Protein | T84, T281 (Engineered glycosylation site DTA was created at two positions F83D and N282A) |
| Glycosite(s) Annotated Protein Sequence | >tr|Q5LH32|Q5LH32_BACFN Putative alpha-L-fucosidase OS=Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) GN=BF0810 PE=4 SV=1
MKNNRLIITLIALFLLGFGLKAQTASTEETAAQKEKRMEWFAQAKLGIFIHWGIYAVNGV
SESWSFFNNYLPYEEYMAQEKGDT*(84)ASAYNPQEWVKLIKESGARYTVITTKHHDGVALWDT
KAGDLSTVKSTPAGRDLIAPFVKEVRKQGLKLGFYYSLLDWSHPDYPNKTRTEVRYKNDP
DRWAKFVKFNFGQLSELNKTWKPDLYWFDGDWEQTAEAWDSKGIINLLRSTNPNVIVNSR
IQGYGDYATPEQGVPVVRPADKYWELCMTMNDSWGYQHADT*(281)AYKTPFMLLRTFVDCLSMG
GNLLLDIGPKEDGTIPAEQIAVLKEFGRWTKKHKEAIYETRAGIPCEHFQGYTTLNKAGD
ILYLYLPYKPNGPIEVKGLVNKVNRVWVVGNGAMLPYKVYNKNYWSEVPGNLYIDIPERV
QDEQITVIAVLLDGPIKLYRGVGQVIESN |
| Sequence Around Glycosites (21 AA) | EEYMAQEKGFTASAYNPQEWV
NDSWGYQHADTNYKTPFMLLR
|
| Glycosite Sequence Logo | seqlogo |
| Glycosite Sequence Logo |  |
| Technique(s) used for Glycosylation Detection | Coomassie staining, Pro-Q Emerald glycostaining, and reactivity with anti-glycan serum |
| Technique(s) used for Glycosylated Residue(s) Detection | Known residues are mutated to create glycosylation motif/sequon |
| Protein Glycosylation- Implication | |
| Glycan Information |
| Glycan Annotation | Exogenous fucose. |
| Technique(s) used for Glycan Identification | Lectin (AAL)binding |
| Protein Glycosylation linked (PGL) gene(s) |
| OST Gene Name | |
| OST NCBI Gene ID | |
| OST GenBank Gene Sequence | |
| OST Protein Name | |
| OST UniProtKB/ SwissProt ID | |
| OST NCBI RefSeq | |
| OST EMBL-CDS | |
| OST UniProtKB Sequence | |
| OST EC Number (BRENDA) | |
| OST Genome Context | |
| Characterized Accessory Gene(s) | |
| PGL Additional Links | CAZy |
| Literatures |
| Reference(s) | 1) Fletcher, C.M., Coyne, M.J. and Comstock, L.E. (2011) Theoretical and experimental characterization of the scope of protein O-glycosylation in Bacteroides fragilis. J Biol Chem, 286, 3219-3226. [PubMed: 21115495] |
| Additional Comments | Engineered glycoprotein.
An example of engineered glycoprotein from a native protein.
Glycosylation sequon features: the sequon has an aspartate (D) preceding the glycosylated T or S which is followed by an amino acid with one or more methyl groups (alanine, isoleucine, or leucine; (D)(S/T)(A/I/L/V/M/T). Moreover, none of the 17 unglycosylated S and T residues examined in BF2494 (excluding two in the signal peptide) have a preceding D, although seven are followed by A, I, or L and one |
| Year of Identification | 2011 |
| Year of Validation | 2011 |
