ProGlyProt ID BC107 Organism Information Organism Name Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) Domain Bacteria Classification Family: Bacteroidaceae Order: "Bacteroidales" Class: "Bacteroidia" Division or phylum: "Bacteroidetes" Taxonomic ID (NCBI) 272559 Genome Sequence (s) GeneBank CR626927.1 EMBL CR626927 Gene Information Gene Name BF0810  NCBI Gene ID 3288800 GenBank Gene Sequence 3288800 Protein Information Protein Name BF0810 UniProtKB/SwissProt ID Q5LH32 NCBI RefSeq YP_210506.1 EMBL-CDS CAH06554.1 UniProtKB Sequence >tr|Q5LH32|Q5LH32_BACFN Putative alpha-L-fucosidase OS=Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) GN=BF0810 PE=4 SV=1 MKNNRLIITLIALFLLGFGLKAQTASTEETAAQKEKRMEWFAQAKLGIFIHWGIYAVNGV SESWSFFNNYLPYEEYMAQEKGFTASAYNPQEWVKLIKESGARYTVITTKHHDGVALWDT KAGDLSTVKSTPAGRDLIAPFVKEVRKQGLKLGFYYSLLDWSHPDYPNKTRTEVRYKNDP DRWAKFVKFNFGQLSELNKTWKPDLYWFDGDWEQTAEAWDSKGIINLLRSTNPNVIVNSR IQGYGDYATPEQGVPVVRPADKYWELCMTMNDSWGYQHADTNYKTPFMLLRTFVDCLSMG GNLLLDIGPKEDGTIPAEQIAVLKEFGRWTKKHKEAIYETRAGIPCEHFQGYTTLNKAGD ILYLYLPYKPNGPIEVKGLVNKVNRVWVVGNGAMLPYKVYNKNYWSEVPGNLYIDIPERV QDEQITVIAVLLDGPIKLYRGVGQVIESN Sequence length 449 AA Subcellular Location Function Putative alpha-L-fucosidase. Protein Structure PDB ID Glycosylation Status Glycosylation Type O (Thr) linked Experimentally Validated Glycosite(s) in Full Length Protein T84, T281 (Engineered glycosylation site DTA was created at two positions F83D and N282A) Experimentally Validated Glycosite(s ) in Mature Protein T84, T281 (Engineered glycosylation site DTA was created at two positions F83D and N282A) Glycosite(s) Annotated Protein Sequence >tr|Q5LH32|Q5LH32_BACFN Putative alpha-L-fucosidase OS=Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) GN=BF0810 PE=4 SV=1 MKNNRLIITLIALFLLGFGLKAQTASTEETAAQKEKRMEWFAQAKLGIFIHWGIYAVNGV SESWSFFNNYLPYEEYMAQEKGDT*(84)ASAYNPQEWVKLIKESGARYTVITTKHHDGVALWDT KAGDLSTVKSTPAGRDLIAPFVKEVRKQGLKLGFYYSLLDWSHPDYPNKTRTEVRYKNDP DRWAKFVKFNFGQLSELNKTWKPDLYWFDGDWEQTAEAWDSKGIINLLRSTNPNVIVNSR IQGYGDYATPEQGVPVVRPADKYWELCMTMNDSWGYQHADT*(281)AYKTPFMLLRTFVDCLSMG GNLLLDIGPKEDGTIPAEQIAVLKEFGRWTKKHKEAIYETRAGIPCEHFQGYTTLNKAGD ILYLYLPYKPNGPIEVKGLVNKVNRVWVVGNGAMLPYKVYNKNYWSEVPGNLYIDIPERV QDEQITVIAVLLDGPIKLYRGVGQVIESN Sequence Around Glycosites (21 AA) EEYMAQEKGFTASAYNPQEWV NDSWGYQHADTNYKTPFMLLR Glycosite Sequence Logo seqlogo Glycosite Sequence Logo Technique(s) used for Glycosylation Detection Coomassie staining, Pro-Q Emerald glycostaining, and reactivity with anti-glycan serum Technique(s) used for Glycosylated Residue(s) Detection Known residues are mutated to create glycosylation motif/sequon Protein Glycosylation- Implication Glycan Information Glycan Annotation Exogenous fucose. Technique(s) used for Glycan Identification Lectin (AAL)binding Protein Glycosylation linked (PGL) gene(s) OST Gene Name OST NCBI Gene ID OST GenBank Gene Sequence OST Protein Name OST UniProtKB/ SwissProt ID OST NCBI RefSeq OST EMBL-CDS OST UniProtKB Sequence OST EC Number (BRENDA) OST Genome Context Characterized Accessory Gene(s) PGL Additional Links CAZy Literatures Reference(s) 1) Fletcher, C.M., Coyne, M.J. and Comstock, L.E. (2011) Theoretical and experimental characterization of the scope of protein O-glycosylation in Bacteroides fragilis. J Biol Chem, 286, 3219-3226. [PubMed: 21115495] Additional Comments Engineered glycoprotein. An example of engineered glycoprotein from a native protein. Glycosylation sequon features: the sequon has an aspartate (D) preceding the glycosylated T or S which is followed by an amino acid with one or more methyl groups (alanine, isoleucine, or leucine; (D)(S/T)(A/I/L/V/M/T). Moreover, none of the 17 unglycosylated S and T residues examined in BF2494 (excluding two in the signal peptide) have a preceding D, although seven are followed by A, I, or L and one Year of Identification 2011 Year of Validation 2011