ProGlyProt IDBC106
Organism Information
Organism NameBacillus subtilis 168
DomainBacteria
ClassificationFamily: Bacillaceae
Order: Bacillales
Class: Bacilli (or Firmibacteria)
Division or phylum: "Firmicutes"
Taxonomic ID (NCBI)1423
Genome Sequence (s)
GeneBankAF014938.1
EMBLAF014938
Gene Information
Gene NamesunA
NCBI Gene ID939121
GenBank Gene Sequence939121
Protein Information
Protein NameSublansin
UniProtKB/SwissProt IDP68577
NCBI RefSeqNP_390031.1
EMBL-CDSAAC63531.1
UniProtKB Sequence>sp|P68577|SUNA_BACSU SPBc2 prophage-derived bacteriocin sublancin-168 OS=Bacillus subtilis GN=sunA PE=1 SV=1 MEKLFKEVKLEELENQKGSGLGKAQCAALWLQCASGGTIGCGGGAVACQNYRQFCR
Sequence length56 AA
Subcellular LocationSecreted
FunctionSPβ prophage-derived bacteriocin sublancin-168. It has antimicrobial activity against Gram-positive bacteria. It is stable at both low and high pH and lacks free thiols.
Protein Structure
PDB ID
Glycosylation Status
Glycosylation TypeS (Cys) linked
Experimentally Validated Glycosite(s) in Full Length Protein(Propeptide: 1-19) C41
Experimentally Validated Glycosite(s ) in Mature ProteinC22
Glycosite(s) Annotated Protein Sequence>sp|P68577|SUNA_BACSU SPBc2 prophage-derived bacteriocin sublancin-168 OS=Bacillus subtilis GN=sunA PE=1 SV=1 MEKLFKEVKLEELENQKGSGLGKAQCAALWLQCASGGTIGC*(41)GGGAVACQNYRQFCR
Sequence Around Glycosites (21 AA)LQCASGGTIGCGGGAVACQNY
Glycosite Sequence Logoseqlogo
Glycosite Sequence Logo
Technique(s) used for Glycosylation DetectionHigher mass observed using mass spectrometry
Technique(s) used for Glycosylated Residue(s) DetectionTandem ESI-MS (electrospray ionization quadrupole-TOF mass spectrometry) analysis after chymotrypsin digestion.
Protein Glycosylation- ImplicationGlucosylation is essential for its bioactivity.
Glycan Information
Glycan AnnotationUDP-Glc, UDP-GlcNAc, UDP-Gal, GDP-Man and UDP-Xyl can serve as substrates for SunS GTase but UDP-α-D-glucose is most efficiently used.
Technique(s) used for Glycan IdentificationGC-MS (gas chromatography-mass spectrometry) analysis after trimethylsilylation
Protein Glycosylation linked (PGL) gene(s)
OST Gene Name
OST NCBI Gene ID
OST GenBank Gene Sequence
OST Protein Name
OST UniProtKB/ SwissProt ID
OST NCBI RefSeq
OST EMBL-CDS
OST UniProtKB Sequence
OST EC Number (BRENDA)
OST Genome Context
Characterized Accessory Gene(s)SunS is a glycosyltransferase with very relaxed substrate specificity. It shows strong regioselectivity and chemoselectivity for glycosylation of a thiol.
PGL Additional LinksCAZy
Literatures
Reference(s)1) Oman, T.J., Boettcher, J.M., Wang, H., Okalibe, X.N. and van der Donk, W.A. (2011) Sublancin is not a lantibiotic but an S-linked glycopeptide. Nat Chem Biol, 7, 78-80. [PubMed: 21196935]
2) Stepper, J., Shastri, S., Loo, T.S., Preston, J.C., Novak, P., Man, P., Moore, C.H., Havlicek, V., Patchett, M.L. and Norris, G.E. (2011) Cysteine S-glycosylation, a new post-translational modification found in glycopeptide bacteriocins. FEBS Lett, 585, 645-650. [PubMed: 21251913]
Additional CommentsS-linked glycosylation is very rare. It has been observed that cysteine glycosylation leads to the formation of more stable products (both at low and high pH) than does serine glycosylation.
Year of Identification2011
Year of Validation2011