| ProGlyProt ID | BC104 |
| Organism Information |
| Organism Name | Aneurinibacillus thermoaerophilus L420-91T (DSM 10154) and GS4-97 |
| Domain | Bacteria |
| Classification | Family: Paenibacillaceae
Order: Bacillales
Class: Bacilli (or Firmibacteria)
Division or phylum: "Firmicutes" |
| Taxonomic ID (NCBI) | 143495 |
| Genome Sequence (s) |
| EMBL | AY395578 |
| Gene Information |
| Gene Name | sat A |
| NCBI Gene ID | |
| Protein Information |
| Protein Name | Surface-layer glycoprotein SatA |
| UniProtKB/SwissProt ID | Q6TL22 |
| NCBI RefSeq | |
| EMBL-CDS | AAS44591.1 |
| UniProtKB Sequence | >tr|Q6TL22|Q6TL22_ANETH Surface-layer glycoprotein SatA OS=Aneurinibacillus thermoaerophilus GN=satA PE=3 SV=1
MKTNKALKVLTTSALLASVAAPFGAGVVSANSTNLVDKRVQVGSNSEINGTSHLIVKEDD
KKFRVSTGDTFRVELPAGTEWKFSDSEFYVDGSGNVYSSESSASPDTKAKVNVISSRTIE
VRPLGNGFKSGDPDSKNAINIPLHVKVNGASGELKLKIDSMNSTVTSGEYVFATVNTGNT
RAVVDTVRYIGKNGIGGQIRIDENFAGAISAGTHSLKLKLPSNFEWDDMSSGEIKFGGSF
FGNAKVDGAIIGNGDNTLTIPVKISQSTSGATPGYIYITPKIKAKSDAAKGTDVTVNISG
GDFSDADLTVAKYGDYDANIKVEKVEELVAGKFDEYTKTAKITIEESVPGSFYKDRDIDV
KFPSWVKVVEVSDWSTSGVKANKPKNIDGTDDEFSIKIEEPTTKENTTPGRIEFKVRLSI
DANKTGDIEATVSGGGIKEQKAVVAKAIAPAAVSVEGDTSKLKIGAQDQEGPTFYITEAK
KGVFEKNVEGPSGNGEIIVRLASGVKFSKVPKVEVVEGDGPIDTSNVRRIENDNALAIPI
KGESTSKPMKLKVSDVRLTLDRTVPEGDIEAKIGGSAVVENYVKDSKGWVGTGLYTGTDN
KNPGTFNTQHAVTFKIGSTITPAPSDTTAKNIVFKLNSKTYTVDGKEIEMDAAPLVGWDR
AYLPVRFAANALNVSDDQIIWDDKTSTFTIFKGDRVISGKVGDKFLTINGVKTPMDVPVW
RNKEKTNNRVMVPIRYLANALNAEINWNKETSEITIQVK
|
| Sequence length | 759 AA |
| Subcellular Location | Surface |
| Function | |
| Protein Structure |
| PDB ID | |
| Glycosylation Status |
| Glycosylation Type | O (Ser/Thr) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | T67 |
| Experimentally Validated Glycosite(s ) in Mature Protein | T67 |
| Glycosite(s) Annotated Protein Sequence | >tr|Q6TL22|Q6TL22_ANETH Surface-layer glycoprotein SatA OS=Aneurinibacillus thermoaerophilus GN=satA PE=3 SV=1
MKTNKALKVLTTSALLASVAAPFGAGVVSANSTNLVDKRVQVGSNSEINGTSHLIVKEDD
KKFRVST*(67)GDTFRVELPAGTEWKFSDSEFYVDGSGNVYSSESSASPDTKAKVNVISSRTIE
VRPLGNGFKSGDPDSKNAINIPLHVKVNGASGELKLKIDSMNSTVTSGEYVFATVNTGNT
RAVVDTVRYIGKNGIGGQIRIDENFAGAISAGTHSLKLKLPSNFEWDDMSSGEIKFGGSF
FGNAKVDGAIIGNGDNTLTIPVKISQSTSGATPGYIYITPKIKAKSDAAKGTDVTVNISG
GDFSDADLTVAKYGDYDANIKVEKVEELVAGKFDEYTKTAKITIEESVPGSFYKDRDIDV
KFPSWVKVVEVSDWSTSGVKANKPKNIDGTDDEFSIKIEEPTTKENTTPGRIEFKVRLSI
DANKTGDIEATVSGGGIKEQKAVVAKAIAPAAVSVEGDTSKLKIGAQDQEGPTFYITEAK
KGVFEKNVEGPSGNGEIIVRLASGVKFSKVPKVEVVEGDGPIDTSNVRRIENDNALAIPI
KGESTSKPMKLKVSDVRLTLDRTVPEGDIEAKIGGSAVVENYVKDSKGWVGTGLYTGTDN
KNPGTFNTQHAVTFKIGSTITPAPSDTTAKNIVFKLNSKTYTVDGKEIEMDAAPLVGWDR
AYLPVRFAANALNVSDDQIIWDDKTSTFTIFKGDRVISGKVGDKFLTINGVKTPMDVPVW
RNKEKTNNRVMVPIRYLANALNAEINWNKETSEITIQVK
|
| Sequence Around Glycosites (21 AA) | KEDDKKFRVSTGDTFRVELPA |
| Glycosite Sequence Logo | seqlogo |
| Glycosite Sequence Logo |  |
| Technique(s) used for Glycosylation Detection | Periodic acid-Schiff (PAS)-staining |
| Technique(s) used for Glycosylated Residue(s) Detection | N-terminal sequencing and C-13 NMR |
| Protein Glycosylation- Implication | |
| Glycan Information |
| Glycan Annotation | Linkage: β-GalNAc-Ser/Thr.
Hexasaccharide repeating unit (an average of 15 repeats) with the constituents D-rhamnose and 3-N-acetyl-D-fucosamine (3-acetamido-3,6-dideoxy-D-galactose); and β-D-GalNAc-Ser/Thr linkage. An O-methyl group on the non-reducing terminal rhamnose. |
| Technique(s) used for Glycan Identification | |
| Protein Glycosylation linked (PGL) gene(s) |
| OST Gene Name | |
| OST NCBI Gene ID | |
| OST GenBank Gene Sequence | |
| OST Protein Name | |
| OST UniProtKB/ SwissProt ID | |
| OST NCBI RefSeq | |
| OST EMBL-CDS | |
| OST UniProtKB Sequence | |
| OST EC Number (BRENDA) | |
| OST Genome Context | |
| Characterized Accessory Gene(s) | |
| PGL Additional Links | CAZy |
| Literatures |
| Reference(s) | 1) Messner, P., Steiner, K., Zarschler, K. and Schaffer, C. (2008) S-layer nanoglycobiology of bacteria. Carbohydr Res, 343, 1934-1951. [PubMed: 18336801]
2) Pfoestl, A., Hofinger, A., Kosma, P. and Messner, P. (2003) Biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose in Aneurinibacillus thermoaerophilus L420-91T. J Biol Chem, 278, 26410-26417. [PubMed: 12740380]
3) Schaffer, C., Muller, N., Christian, R., Graninger, M., Wugeditsch, T., Scheberl, A. and Messner, P. (1999) C |
| Additional Comments | |
| Year of Identification | 1995 |
| Year of Validation | 1999 |
