ProGlyProt ID BC104 Organism Information Organism Name Aneurinibacillus thermoaerophilus L420-91T (DSM 10154) and GS4-97 Domain Bacteria Classification Family: Paenibacillaceae Order: Bacillales Class: Bacilli (or Firmibacteria) Division or phylum: "Firmicutes" Taxonomic ID (NCBI) 143495 Genome Sequence (s) EMBL AY395578 Gene Information Gene Name sat A NCBI Gene ID Protein Information Protein Name Surface-layer glycoprotein SatA UniProtKB/SwissProt ID Q6TL22 NCBI RefSeq EMBL-CDS AAS44591.1 UniProtKB Sequence >tr|Q6TL22|Q6TL22_ANETH Surface-layer glycoprotein SatA OS=Aneurinibacillus thermoaerophilus GN=satA PE=3 SV=1 MKTNKALKVLTTSALLASVAAPFGAGVVSANSTNLVDKRVQVGSNSEINGTSHLIVKEDD KKFRVSTGDTFRVELPAGTEWKFSDSEFYVDGSGNVYSSESSASPDTKAKVNVISSRTIE VRPLGNGFKSGDPDSKNAINIPLHVKVNGASGELKLKIDSMNSTVTSGEYVFATVNTGNT RAVVDTVRYIGKNGIGGQIRIDENFAGAISAGTHSLKLKLPSNFEWDDMSSGEIKFGGSF FGNAKVDGAIIGNGDNTLTIPVKISQSTSGATPGYIYITPKIKAKSDAAKGTDVTVNISG GDFSDADLTVAKYGDYDANIKVEKVEELVAGKFDEYTKTAKITIEESVPGSFYKDRDIDV KFPSWVKVVEVSDWSTSGVKANKPKNIDGTDDEFSIKIEEPTTKENTTPGRIEFKVRLSI DANKTGDIEATVSGGGIKEQKAVVAKAIAPAAVSVEGDTSKLKIGAQDQEGPTFYITEAK KGVFEKNVEGPSGNGEIIVRLASGVKFSKVPKVEVVEGDGPIDTSNVRRIENDNALAIPI KGESTSKPMKLKVSDVRLTLDRTVPEGDIEAKIGGSAVVENYVKDSKGWVGTGLYTGTDN KNPGTFNTQHAVTFKIGSTITPAPSDTTAKNIVFKLNSKTYTVDGKEIEMDAAPLVGWDR AYLPVRFAANALNVSDDQIIWDDKTSTFTIFKGDRVISGKVGDKFLTINGVKTPMDVPVW RNKEKTNNRVMVPIRYLANALNAEINWNKETSEITIQVK Sequence length 759 AA Subcellular Location Surface Function Protein Structure PDB ID Glycosylation Status Glycosylation Type O (Ser/Thr) linked Experimentally Validated Glycosite(s) in Full Length Protein T67 Experimentally Validated Glycosite(s ) in Mature Protein T67 Glycosite(s) Annotated Protein Sequence >tr|Q6TL22|Q6TL22_ANETH Surface-layer glycoprotein SatA OS=Aneurinibacillus thermoaerophilus GN=satA PE=3 SV=1 MKTNKALKVLTTSALLASVAAPFGAGVVSANSTNLVDKRVQVGSNSEINGTSHLIVKEDD KKFRVST*(67)GDTFRVELPAGTEWKFSDSEFYVDGSGNVYSSESSASPDTKAKVNVISSRTIE VRPLGNGFKSGDPDSKNAINIPLHVKVNGASGELKLKIDSMNSTVTSGEYVFATVNTGNT RAVVDTVRYIGKNGIGGQIRIDENFAGAISAGTHSLKLKLPSNFEWDDMSSGEIKFGGSF FGNAKVDGAIIGNGDNTLTIPVKISQSTSGATPGYIYITPKIKAKSDAAKGTDVTVNISG GDFSDADLTVAKYGDYDANIKVEKVEELVAGKFDEYTKTAKITIEESVPGSFYKDRDIDV KFPSWVKVVEVSDWSTSGVKANKPKNIDGTDDEFSIKIEEPTTKENTTPGRIEFKVRLSI DANKTGDIEATVSGGGIKEQKAVVAKAIAPAAVSVEGDTSKLKIGAQDQEGPTFYITEAK KGVFEKNVEGPSGNGEIIVRLASGVKFSKVPKVEVVEGDGPIDTSNVRRIENDNALAIPI KGESTSKPMKLKVSDVRLTLDRTVPEGDIEAKIGGSAVVENYVKDSKGWVGTGLYTGTDN KNPGTFNTQHAVTFKIGSTITPAPSDTTAKNIVFKLNSKTYTVDGKEIEMDAAPLVGWDR AYLPVRFAANALNVSDDQIIWDDKTSTFTIFKGDRVISGKVGDKFLTINGVKTPMDVPVW RNKEKTNNRVMVPIRYLANALNAEINWNKETSEITIQVK Sequence Around Glycosites (21 AA) KEDDKKFRVSTGDTFRVELPA Glycosite Sequence Logo seqlogo Glycosite Sequence Logo Technique(s) used for Glycosylation Detection Periodic acid-Schiff (PAS)-staining Technique(s) used for Glycosylated Residue(s) Detection N-terminal sequencing and C-13 NMR Protein Glycosylation- Implication Glycan Information Glycan Annotation Linkage: β-GalNAc-Ser/Thr. Hexasaccharide repeating unit (an average of 15 repeats) with the constituents D-rhamnose and 3-N-acetyl-D-fucosamine (3-acetamido-3,6-dideoxy-D-galactose); and β-D-GalNAc-Ser/Thr linkage. An O-methyl group on the non-reducing terminal rhamnose. Technique(s) used for Glycan Identification Protein Glycosylation linked (PGL) gene(s) OST Gene Name OST NCBI Gene ID OST GenBank Gene Sequence OST Protein Name OST UniProtKB/ SwissProt ID OST NCBI RefSeq OST EMBL-CDS OST UniProtKB Sequence OST EC Number (BRENDA) OST Genome Context Characterized Accessory Gene(s) PGL Additional Links CAZy Literatures Reference(s) 1) Messner, P., Steiner, K., Zarschler, K. and Schaffer, C. (2008) S-layer nanoglycobiology of bacteria. Carbohydr Res, 343, 1934-1951. [PubMed: 18336801] 2) Pfoestl, A., Hofinger, A., Kosma, P. and Messner, P. (2003) Biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose in Aneurinibacillus thermoaerophilus L420-91T. J Biol Chem, 278, 26410-26417. [PubMed: 12740380] 3) Schaffer, C., Muller, N., Christian, R., Graninger, M., Wugeditsch, T., Scheberl, A. and Messner, P. (1999) C Additional Comments Year of Identification 1995 Year of Validation 1999