| ProGlyProt ID | BC103 |
| Organism Information |
| Organism Name | Aneurinibacillus thermoaerophilus DSM 10155 |
| Domain | Bacteria |
| Classification | Family: Paenibacillaceae
Order: Bacillales
Class: Bacilli (or Firmibacteria)
Division or phylum: "Firmicutes" |
| Taxonomic ID (NCBI) | 143495 |
| Genome Sequence (s) |
| EMBL | AY395579 |
| Gene Information |
| Gene Name | sat B |
| NCBI Gene ID | |
| Protein Information |
| Protein Name | Surface-layer glycoprotein Sat B |
| UniProtKB/SwissProt ID | Q6TL21 |
| NCBI RefSeq | |
| EMBL-CDS | AAS44592.1 |
| UniProtKB Sequence | >tr|Q6TL21|Q6TL21_ANETH Surface-layer glycoprotein SatB OS=Aneurinibacillus thermoaerophilus GN=satB PE=3 SV=1
MKTNKALKVLTTSALLASVAAPFGAGVVSANITNLVNKVLNVKSNYDGEQSASHTLTVKE
DDIAFDQTSGETFRVVLPSGVKWKTAPANTPTAKFDLRSDQVLEVTVKGTNTIADDEQVS
IPLNFKVDGATGELKLKVESMDSTITSGEYVFAIVGSGSTTAAVDNVKNIGKSGKAGTIR
VEETAIGAVADGRNHKVKLKLPSNFRWVTTGTDKTEISTSGGFTSASIVTGESNDNVLVL
DVATATGQGRGVLYVTPYIKADKDASYGDVSVNIRGPDFSDSDVVVAKYGDWGATIKVEK
VEEVLAGKYDENTRTAKITIEESVPGSFVKGRDIDVELPSWVKVTDIKEFTTSGFTTGAP
TSGSVDIDGTDNKISIPLGETGGSSTAKIEFKLELSVQADKTGDIEAVISGGGVAEQKAV
IAKAVAPVSVALDGEASKVKIGAQAQDAPNIIITELKKGAIEKTTENGSTTNGTIEVKLP
DGVKFASVPKVEVVEGDGTIDVDNVRRINNDNVLTIPVKGESVSKPMKIKLSNVKLTVDR
TVPEGDIELKVGGNAIVENYKSGKGWLKGNKQNGSSDALDAGEFNTEHAAKVVVAKTVTP
APTDTTATNIVFKLNSKTYTVDGKEFEMDAAPLVGWDRAYLPVRFAANALNVSDDQIVWD
DKTSTFTIFKGDRVISGKVGDKFLTVNGAKVPMDVPVWRNKEKTNNRVMVPIRYLANALN
AEIEWNKETSEITIQAKK
|
| Sequence length | 738 AA |
| Subcellular Location | Surface |
| Function | |
| Protein Structure |
| PDB ID | |
| Glycosylation Status |
| Glycosylation Type | O (Ser/Thr) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | T471 and /or S69
|
| Experimentally Validated Glycosite(s ) in Mature Protein | T471 and /or S69
|
| Glycosite(s) Annotated Protein Sequence | >tr|Q6TL21|Q6TL21_ANETH Surface-layer glycoprotein SatB OS=Aneurinibacillus thermoaerophilus GN=satB PE=3 SV=1
MKTNKALKVLTTSALLASVAAPFGAGVVSANITNLVNKVLNVKSNYDGEQSASHTLTVKE
DDIAFDQTS*(69)GETFRVVLPSGVKWKTAPANTPTAKFDLRSDQVLEVTVKGTNTIADDEQVS
IPLNFKVDGATGELKLKVESMDSTITSGEYVFAIVGSGSTTAAVDNVKNIGKSGKAGTIR
VEETAIGAVADGRNHKVKLKLPSNFRWVTTGTDKTEISTSGGFTSASIVTGESNDNVLVL
DVATATGQGRGVLYVTPYIKADKDASYGDVSVNIRGPDFSDSDVVVAKYGDWGATIKVEK
VEEVLAGKYDENTRTAKITIEESVPGSFVKGRDIDVELPSWVKVTDIKEFTTSGFTTGAP
TSGSVDIDGTDNKISIPLGETGGSSTAKIEFKLELSVQADKTGDIEAVISGGGVAEQKAV
IAKAVAPVSVALDGEASKVKIGAQAQDAPNIIITELKKGAIEKTTENGSTT*(471)NGTIEVKLP
DGVKFASVPKVEVVEGDGTIDVDNVRRINNDNVLTIPVKGESVSKPMKIKLSNVKLTVDR
TVPEGDIELKVGGNAIVENYKSGKGWLKGNKQNGSSDALDAGEFNTEHAAKVVVAKTVTP
APTDTTATNIVFKLNSKTYTVDGKEFEMDAAPLVGWDRAYLPVRFAANALNVSDDQIVWD
DKTSTFTIFKGDRVISGKVGDKFLTVNGAKVPMDVPVWRNKEKTNNRVMVPIRYLANALN
AEIEWNKETSEITIQAKK
|
| Sequence Around Glycosites (21 AA) | IEKTTENGSTTNGTIEVKLPD
KEDDIAFDQTSGETFRVVLPS
|
| Glycosite Sequence Logo | seqlogo |
| Glycosite Sequence Logo |  |
| Technique(s) used for Glycosylation Detection | Periodic acid-Schiff (PAS) staining |
| Technique(s) used for Glycosylated Residue(s) Detection | Modified Edman degradation |
| Protein Glycosylation- Implication | |
| Glycan Information |
| Glycan Annotation | Linkage: β-GalNAc-Ser/Thr.
Total carbohydrate content of 15%.
Disaccharide repeating unit composed of L-rhamnose and D-glycero-D-manno-heptose residues: →4)-α-L-Rhap-(1→3)-β-D-glycero-D-manno-Hepp-(1→ and β-D-GalpNAc-(1→O)-Ser/Thr linkage. Equal amounts of the complete core structure α-L-Rhap-(1→3)-α-L-Rhap-(1→3)-β-D-GalpNAc-(1→O)-Ser/Thr and the truncated forms α-L-Rhap-(1→3)-β-D-GalpNAc-(1→O)-Ser/Thr and β-D-GalpNAc-(1→O)-Ser/Thr are present. The differe |
| Technique(s) used for Glycan Identification | |
| Protein Glycosylation linked (PGL) gene(s) |
| OST Gene Name | |
| OST NCBI Gene ID | |
| OST GenBank Gene Sequence | |
| OST Protein Name | |
| OST UniProtKB/ SwissProt ID | |
| OST NCBI RefSeq | |
| OST EMBL-CDS | |
| OST UniProtKB Sequence | |
| OST EC Number (BRENDA) | |
| OST Genome Context | |
| Characterized Accessory Gene(s) | |
| PGL Additional Links | CAZy |
| Literatures |
| Reference(s) | 1) Messner, P., Steiner, K., Zarschler, K. and Schaffer, C. (2008) S-layer nanoglycobiology of bacteria. Carbohydr Res, 343, 1934-1951. [PubMed: 18336801]
2) Kneidinger, B., Graninger, M., Puchberger, M., Kosma, P. and Messner, P. (2001) Biosynthesis of nucleotide-activated D-glycero-D-manno-heptose. J Biol Chem, 276, 20935-20944. [PubMed: 11279237]
3) Wugeditsch, T., Zachara, N.E., Puchberger, M., Kosma, P., Gooley, A.A. and Messner, P. (1999) Structural heterogeneity in the core olig |
| Additional Comments | |
| Year of Identification | 1995 |
| Year of Validation | 1999 |
