ProGlyProt ID BC103 Organism Information Organism Name Aneurinibacillus thermoaerophilus DSM 10155 Domain Bacteria Classification Family: Paenibacillaceae Order: Bacillales Class: Bacilli (or Firmibacteria) Division or phylum: "Firmicutes" Taxonomic ID (NCBI) 143495 Genome Sequence (s) EMBL AY395579 Gene Information Gene Name sat B NCBI Gene ID Protein Information Protein Name Surface-layer glycoprotein Sat B UniProtKB/SwissProt ID Q6TL21 NCBI RefSeq EMBL-CDS AAS44592.1 UniProtKB Sequence >tr|Q6TL21|Q6TL21_ANETH Surface-layer glycoprotein SatB OS=Aneurinibacillus thermoaerophilus GN=satB PE=3 SV=1 MKTNKALKVLTTSALLASVAAPFGAGVVSANITNLVNKVLNVKSNYDGEQSASHTLTVKE DDIAFDQTSGETFRVVLPSGVKWKTAPANTPTAKFDLRSDQVLEVTVKGTNTIADDEQVS IPLNFKVDGATGELKLKVESMDSTITSGEYVFAIVGSGSTTAAVDNVKNIGKSGKAGTIR VEETAIGAVADGRNHKVKLKLPSNFRWVTTGTDKTEISTSGGFTSASIVTGESNDNVLVL DVATATGQGRGVLYVTPYIKADKDASYGDVSVNIRGPDFSDSDVVVAKYGDWGATIKVEK VEEVLAGKYDENTRTAKITIEESVPGSFVKGRDIDVELPSWVKVTDIKEFTTSGFTTGAP TSGSVDIDGTDNKISIPLGETGGSSTAKIEFKLELSVQADKTGDIEAVISGGGVAEQKAV IAKAVAPVSVALDGEASKVKIGAQAQDAPNIIITELKKGAIEKTTENGSTTNGTIEVKLP DGVKFASVPKVEVVEGDGTIDVDNVRRINNDNVLTIPVKGESVSKPMKIKLSNVKLTVDR TVPEGDIELKVGGNAIVENYKSGKGWLKGNKQNGSSDALDAGEFNTEHAAKVVVAKTVTP APTDTTATNIVFKLNSKTYTVDGKEFEMDAAPLVGWDRAYLPVRFAANALNVSDDQIVWD DKTSTFTIFKGDRVISGKVGDKFLTVNGAKVPMDVPVWRNKEKTNNRVMVPIRYLANALN AEIEWNKETSEITIQAKK Sequence length 738 AA Subcellular Location Surface Function Protein Structure PDB ID Glycosylation Status Glycosylation Type O (Ser/Thr) linked Experimentally Validated Glycosite(s) in Full Length Protein T471 and /or S69 Experimentally Validated Glycosite(s ) in Mature Protein T471 and /or S69 Glycosite(s) Annotated Protein Sequence >tr|Q6TL21|Q6TL21_ANETH Surface-layer glycoprotein SatB OS=Aneurinibacillus thermoaerophilus GN=satB PE=3 SV=1 MKTNKALKVLTTSALLASVAAPFGAGVVSANITNLVNKVLNVKSNYDGEQSASHTLTVKE DDIAFDQTS*(69)GETFRVVLPSGVKWKTAPANTPTAKFDLRSDQVLEVTVKGTNTIADDEQVS IPLNFKVDGATGELKLKVESMDSTITSGEYVFAIVGSGSTTAAVDNVKNIGKSGKAGTIR VEETAIGAVADGRNHKVKLKLPSNFRWVTTGTDKTEISTSGGFTSASIVTGESNDNVLVL DVATATGQGRGVLYVTPYIKADKDASYGDVSVNIRGPDFSDSDVVVAKYGDWGATIKVEK VEEVLAGKYDENTRTAKITIEESVPGSFVKGRDIDVELPSWVKVTDIKEFTTSGFTTGAP TSGSVDIDGTDNKISIPLGETGGSSTAKIEFKLELSVQADKTGDIEAVISGGGVAEQKAV IAKAVAPVSVALDGEASKVKIGAQAQDAPNIIITELKKGAIEKTTENGSTT*(471)NGTIEVKLP DGVKFASVPKVEVVEGDGTIDVDNVRRINNDNVLTIPVKGESVSKPMKIKLSNVKLTVDR TVPEGDIELKVGGNAIVENYKSGKGWLKGNKQNGSSDALDAGEFNTEHAAKVVVAKTVTP APTDTTATNIVFKLNSKTYTVDGKEFEMDAAPLVGWDRAYLPVRFAANALNVSDDQIVWD DKTSTFTIFKGDRVISGKVGDKFLTVNGAKVPMDVPVWRNKEKTNNRVMVPIRYLANALN AEIEWNKETSEITIQAKK Sequence Around Glycosites (21 AA) IEKTTENGSTTNGTIEVKLPD KEDDIAFDQTSGETFRVVLPS Glycosite Sequence Logo seqlogo Glycosite Sequence Logo Technique(s) used for Glycosylation Detection Periodic acid-Schiff (PAS) staining Technique(s) used for Glycosylated Residue(s) Detection Modified Edman degradation Protein Glycosylation- Implication Glycan Information Glycan Annotation Linkage: β-GalNAc-Ser/Thr. Total carbohydrate content of 15%. Disaccharide repeating unit composed of L-rhamnose and D-glycero-D-manno-heptose residues: →4)-α-L-Rhap-(1→3)-β-D-glycero-D-manno-Hepp-(1→ and β-D-GalpNAc-(1→O)-Ser/Thr linkage. Equal amounts of the complete core structure α-L-Rhap-(1→3)-α-L-Rhap-(1→3)-β-D-GalpNAc-(1→O)-Ser/Thr and the truncated forms α-L-Rhap-(1→3)-β-D-GalpNAc-(1→O)-Ser/Thr and β-D-GalpNAc-(1→O)-Ser/Thr are present. The differe Technique(s) used for Glycan Identification Protein Glycosylation linked (PGL) gene(s) OST Gene Name OST NCBI Gene ID OST GenBank Gene Sequence OST Protein Name OST UniProtKB/ SwissProt ID OST NCBI RefSeq OST EMBL-CDS OST UniProtKB Sequence OST EC Number (BRENDA) OST Genome Context Characterized Accessory Gene(s) PGL Additional Links CAZy Literatures Reference(s) 1) Messner, P., Steiner, K., Zarschler, K. and Schaffer, C. (2008) S-layer nanoglycobiology of bacteria. Carbohydr Res, 343, 1934-1951. [PubMed: 18336801] 2) Kneidinger, B., Graninger, M., Puchberger, M., Kosma, P. and Messner, P. (2001) Biosynthesis of nucleotide-activated D-glycero-D-manno-heptose. J Biol Chem, 276, 20935-20944. [PubMed: 11279237] 3) Wugeditsch, T., Zachara, N.E., Puchberger, M., Kosma, P., Gooley, A.A. and Messner, P. (1999) Structural heterogeneity in the core olig Additional Comments Year of Identification 1995 Year of Validation 1999