| ProGlyProt ID | BC102 |
| Organism Information |
| Organism Name | Acidovorax (Pseudomonas) avenae K1 (H8301) |
| Domain | Bacteria |
| Classification | Family: Comamonadaceae
Order: Burkholderiales
Class: Betaproteobacteria
Division or phylum: "Proteobacteria" |
| Taxonomic ID (NCBI) | 80870 |
| Genome Sequence (s) |
| EMBL | AB040140 |
| Gene Information |
| Gene Name | flaA |
| NCBI Gene ID | |
| Protein Information |
| Protein Name | Flagellin |
| UniProtKB/SwissProt ID | Q9FAE7 |
| NCBI RefSeq | |
| EMBL-CDS | BAB16757.1 |
| UniProtKB Sequence | >tr|Q9FAE7|Q9FAE7_9BURK Flagellin OS=Acidovorax avenae subsp. avenae GN=H8301-fla1 PE=4 SV=1
MASTINTNVSSLTAQRNLSLSQSSLNTSIQRLSSGLRINSAKDDAAGLAISERFTSQIRG
LNQAVRNANDGISLAQTAEGALKSTGDILQRVRELAVQSANATNSSGDRKAIQAEVGQLL
SEMDRIAGNTEFNGQKLLDGSFGSATFQVGANANQTITATTGNFRTNNYGAQLTASASGA
ATSGASAGSAGAAAGTVVIAGLQTKTVNVAASGTAADIASAVNAVADSTGVTASARNVSE
LKFSGTGSFTLAVKGDNSTAANVTFNVSATSTAAGLAEAVKAFNDVSSQTGVTAKLNSDS
SGLILTNESGNDINVANGASSAAGITLASQDAATTQSSGTLTFTTATAAGTGATVASRGT
VEYKSDKGYTVSGTGGTMTTTAATSSTLTKVSDIDVSTVDGSTKALKIIDAALSAVNGQR
ASFGALQSRFETTVNNLQSTSENMSASRSRIQDADFAAETANLSRSQILQQAGTAMVAQA
NQLPQGVLSLLK |
| Sequence length | 492 AA |
| Subcellular Location | Surface |
| Function | Flagellin structural protein. |
| Protein Structure |
| PDB ID | |
| Glycosylation Status |
| Glycosylation Type | O (Ser/Thr) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | S178, S183, S212, T351 |
| Experimentally Validated Glycosite(s ) in Mature Protein | S178, S183, S212, T351 |
| Glycosite(s) Annotated Protein Sequence | >tr|Q9FAE7|Q9FAE7_9BURK Flagellin OS=Acidovorax avenae subsp. avenae GN=H8301-fla1 PE=4 SV=1
MASTINTNVSSLTAQRNLSLSQSSLNTSIQRLSSGLRINSAKDDAAGLAISERFTSQIRG
LNQAVRNANDGISLAQTAEGALKSTGDILQRVRELAVQSANATNSSGDRKAIQAEVGQLL
SEMDRIAGNTEFNGQKLLDGSFGSATFQVGANANQTITATTGNFRTNNYGAQLTASAS*(178)GA
ATS*(183)GASAGSAGAAAGTVVIAGLQTKTVNVAAS*(212)GTAADIASAVNAVADSTGVTASARNVSE
LKFSGTGSFTLAVKGDNSTAANVTFNVSATSTAAGLAEAVKAFNDVSSQTGVTAKLNSDS
SGLILTNESGNDINVANGASSAAGITLASQDAATTQSSGTLTFTTATAAGT*(351)GATVASRGT
VEYKSDKGYTVSGTGGTMTTTAATSSTLTKVSDIDVSTVDGSTKALKIIDAALSAVNGQR
ASFGALQSRFETTVNNLQSTSENMSASRSRIQDADFAAETANLSRSQILQQAGTAMVAQA
NQLPQGVLSLLK
|
| Sequence Around Glycosites (21 AA) | NYGAQLTASASGAATSGASAG
LTASASGAATSGASAGSAGAA
LQTKTVNVAASGTAADIASAV
LTFTTATAAGTGATVASRGTV
|
| Glycosite Sequence Logo | seqlogo |
| Glycosite Sequence Logo |  |
| Technique(s) used for Glycosylation Detection | Higher molecular mass detected on SDS-PAGE, Gel code glycoside detection kit (Pierce) |
| Technique(s) used for Glycosylated Residue(s) Detection | HPLC analysis after digestion with trypsin and aspartic N-peptidase (sequencing), site-directed mutagenesis, MALDI-TOF MS (matrix-assisted laser desorption/ionization time of flight mass spectrometry). |
| Protein Glycosylation- Implication | Glycosylation regulates the induction specificity of the immune responses (such as H2O2 generation) to flagellin. It prevents recognition of the flagellin epitope (of virulent strain K1) in rice. K1 flagellin without glycans induces a strong immune response in cultured rice cells. |
| Glycan Information |
| Glycan Annotation | The sites are glycosylated by an approximately 540 Da glycan that may be a derivative of the glycan of P. syringae pv. tabaci. |
| Technique(s) used for Glycan Identification | Mass spectroscopy |
| Protein Glycosylation linked (PGL) gene(s) |
| OST Gene Name | |
| OST NCBI Gene ID | |
| OST GenBank Gene Sequence | |
| OST Protein Name | |
| OST UniProtKB/ SwissProt ID | |
| OST NCBI RefSeq | |
| OST EMBL-CDS | |
| OST UniProtKB Sequence | |
| OST EC Number (BRENDA) | |
| OST Genome Context | |
| Characterized Accessory Gene(s) | |
| PGL Additional Links | CAZy |
| Literatures |
| Reference(s) | 1) Hirai, H., Takai, R., Iwano, M., Nakai, M., Kondo, M., Takayama, S., Isogai, A. and Che, F.S. (2011) Glycosylation regulates the specific induction of rice immune responses by Acidovorax avenae flagellin. J Biol Chem. ahead of print [PMID: 21628471]
2) Che, F.S., Nakajima, Y., Tanaka, N., Iwano, M., Yoshida, T., Takayama, S., Kadota, I. and Isogai, A. (2000) Flagellin from an incompatible strain of Pseudomonas avenae induces a resistance response in cultured rice cells. J Biol Chem, 275, |
| Additional Comments | |
| Year of Identification | 2000 |
| Year of Validation | 2011 |
