ProGlyProt ID BC102 Organism Information Organism Name Acidovorax (Pseudomonas) avenae K1 (H8301) Domain Bacteria Classification Family: Comamonadaceae Order: Burkholderiales Class: Betaproteobacteria Division or phylum: "Proteobacteria" Taxonomic ID (NCBI) 80870 Genome Sequence (s) EMBL AB040140 Gene Information Gene Name flaA NCBI Gene ID Protein Information Protein Name Flagellin UniProtKB/SwissProt ID Q9FAE7 NCBI RefSeq EMBL-CDS BAB16757.1 UniProtKB Sequence >tr|Q9FAE7|Q9FAE7_9BURK Flagellin OS=Acidovorax avenae subsp. avenae GN=H8301-fla1 PE=4 SV=1 MASTINTNVSSLTAQRNLSLSQSSLNTSIQRLSSGLRINSAKDDAAGLAISERFTSQIRG LNQAVRNANDGISLAQTAEGALKSTGDILQRVRELAVQSANATNSSGDRKAIQAEVGQLL SEMDRIAGNTEFNGQKLLDGSFGSATFQVGANANQTITATTGNFRTNNYGAQLTASASGA ATSGASAGSAGAAAGTVVIAGLQTKTVNVAASGTAADIASAVNAVADSTGVTASARNVSE LKFSGTGSFTLAVKGDNSTAANVTFNVSATSTAAGLAEAVKAFNDVSSQTGVTAKLNSDS SGLILTNESGNDINVANGASSAAGITLASQDAATTQSSGTLTFTTATAAGTGATVASRGT VEYKSDKGYTVSGTGGTMTTTAATSSTLTKVSDIDVSTVDGSTKALKIIDAALSAVNGQR ASFGALQSRFETTVNNLQSTSENMSASRSRIQDADFAAETANLSRSQILQQAGTAMVAQA NQLPQGVLSLLK Sequence length 492 AA Subcellular Location Surface Function Flagellin structural protein. Protein Structure PDB ID Glycosylation Status Glycosylation Type O (Ser/Thr) linked Experimentally Validated Glycosite(s) in Full Length Protein S178, S183, S212, T351 Experimentally Validated Glycosite(s ) in Mature Protein S178, S183, S212, T351 Glycosite(s) Annotated Protein Sequence >tr|Q9FAE7|Q9FAE7_9BURK Flagellin OS=Acidovorax avenae subsp. avenae GN=H8301-fla1 PE=4 SV=1 MASTINTNVSSLTAQRNLSLSQSSLNTSIQRLSSGLRINSAKDDAAGLAISERFTSQIRG LNQAVRNANDGISLAQTAEGALKSTGDILQRVRELAVQSANATNSSGDRKAIQAEVGQLL SEMDRIAGNTEFNGQKLLDGSFGSATFQVGANANQTITATTGNFRTNNYGAQLTASAS*(178)GA ATS*(183)GASAGSAGAAAGTVVIAGLQTKTVNVAAS*(212)GTAADIASAVNAVADSTGVTASARNVSE LKFSGTGSFTLAVKGDNSTAANVTFNVSATSTAAGLAEAVKAFNDVSSQTGVTAKLNSDS SGLILTNESGNDINVANGASSAAGITLASQDAATTQSSGTLTFTTATAAGT*(351)GATVASRGT VEYKSDKGYTVSGTGGTMTTTAATSSTLTKVSDIDVSTVDGSTKALKIIDAALSAVNGQR ASFGALQSRFETTVNNLQSTSENMSASRSRIQDADFAAETANLSRSQILQQAGTAMVAQA NQLPQGVLSLLK Sequence Around Glycosites (21 AA) NYGAQLTASASGAATSGASAG LTASASGAATSGASAGSAGAA LQTKTVNVAASGTAADIASAV LTFTTATAAGTGATVASRGTV Glycosite Sequence Logo seqlogo Glycosite Sequence Logo Technique(s) used for Glycosylation Detection Higher molecular mass detected on SDS-PAGE, Gel code glycoside detection kit (Pierce) Technique(s) used for Glycosylated Residue(s) Detection HPLC analysis after digestion with trypsin and aspartic N-peptidase (sequencing), site-directed mutagenesis, MALDI-TOF MS (matrix-assisted laser desorption/ionization time of flight mass spectrometry). Protein Glycosylation- Implication Glycosylation regulates the induction specificity of the immune responses (such as H2O2 generation) to flagellin. It prevents recognition of the flagellin epitope (of virulent strain K1) in rice. K1 flagellin without glycans induces a strong immune response in cultured rice cells. Glycan Information Glycan Annotation The sites are glycosylated by an approximately 540 Da glycan that may be a derivative of the glycan of P. syringae pv. tabaci. Technique(s) used for Glycan Identification Mass spectroscopy Protein Glycosylation linked (PGL) gene(s) OST Gene Name OST NCBI Gene ID OST GenBank Gene Sequence OST Protein Name OST UniProtKB/ SwissProt ID OST NCBI RefSeq OST EMBL-CDS OST UniProtKB Sequence OST EC Number (BRENDA) OST Genome Context Characterized Accessory Gene(s) PGL Additional Links CAZy Literatures Reference(s) 1) Hirai, H., Takai, R., Iwano, M., Nakai, M., Kondo, M., Takayama, S., Isogai, A. and Che, F.S. (2011) Glycosylation regulates the specific induction of rice immune responses by Acidovorax avenae flagellin. J Biol Chem. ahead of print [PMID: 21628471] 2) Che, F.S., Nakajima, Y., Tanaka, N., Iwano, M., Yoshida, T., Takayama, S., Kadota, I. and Isogai, A. (2000) Flagellin from an incompatible strain of Pseudomonas avenae induces a resistance response in cultured rice cells. J Biol Chem, 275, Additional Comments Year of Identification 2000 Year of Validation 2011