ProGlyProt IDBC101
Organism Information
Organism NameAcetogenium kivui (Thermoanaerobacter kivui)
DomainBacteria
ClassificationFamily: Thermoanaerobacteraceae
Order: Thermoanaerobacterales
Class: Clostridia
Division or phylum: "Firmicutes"
Taxonomic ID (NCBI)2325
Genome Sequence (s)
EMBLM31069
Gene Information
Gene Name
NCBI Gene ID
Protein Information
Protein NameCell surface protein/ S-layer protein
UniProtKB/SwissProt IDP22258
NCBI RefSeq
EMBL-CDSAAA21930.1
UniProtKB Sequence>sp|P22258|SLAP_ACEKI Cell surface protein OS=Acetogenium kivui PE=1 SV=1 MKNLKKLIAVVSTFALVFSAMAVGFAATTPFTDVKDDAPYASAVARLYALNITNGVGDPK FGVDQPVTRAQMITFVNRMLGYEDLAEMAKSEKSAFKDVPQNHWAVGQINLAYKLGLAQG VGNGKFDPNSELRYAQALAFVLRALGFKDLDWPYGYLAKAQDLGLVHGLNLAYNGVIKRG DLALILDRALEVPMVKYVDGKEVLGEPLISKVATKAEYTVIATNAQDRSVEEGKVAVLDK DGKLTTINAGLVDFSEYLGKKVIVYSERFGDPVYVAEGDNDVVSFTEGQDSVGTTVYKND DNKTAIKVDDNAYVLYNGYLTKVSKVTVKEGAEVTIINNNYLIVNGSYDNSTIVYNDVQS GDKYLNRDSNYELKGTVTVTGAVSKVTDIKANDYIYYGKQYDVNGNVVGTVIYVVRNQVT GTVTEKSVSGSTYKASIDNVSYTVADNNVWNQLEPGKKVTVILNKDNVIVGISSTTTTTA VNYAIFKEKSDPFTAWFAKVKLILPDAAEKVFDAVYSDVYDKVNLAEGTIVTYTVDANGK LNDIQRANDQPFSSASYKADAKVLTEGSTTYYITDNTVLLNNTSDGYKALKLTDLKDATN LNVKIVADNYNVAKVVVFNNASFVSTTTSTVYAYVTGTADVYVNGSTFTRLTVLENGQTK TYDANAQLATNYTHKAVVLTLTNAKIANIALPTVASGVKLTNIDQANLRITDTTNKGYLL DPNFIVVDTNGNLKGLSDITKDTGVNLYTNDVGKVFVIEIVQ
Sequence length 762 AA
Subcellular LocationSurface
FunctionThe S-layer is a paracrystalline mono-layered assembly of proteins which coat the surface of bacteria. This bacterium is covered by a S-layer with hexagonal symmetry.
Protein Structure
PDB ID
Glycosylation Status
Glycosylation TypeO (Tyr) linked
Experimentally Validated Glycosite(s) in Full Length ProteinY297, Y516, Y520, Y632
Experimentally Validated Glycosite(s ) in Mature ProteinY297, Y516, Y520, Y632
Glycosite(s) Annotated Protein Sequence>sp|P22258|SLAP_ACEKI Cell surface protein OS=Acetogenium kivui PE=1 SV=1 MKNLKKLIAVVSTFALVFSAMAVGFAATTPFTDVKDDAPYASAVARLYALNITNGVGDPK FGVDQPVTRAQMITFVNRMLGYEDLAEMAKSEKSAFKDVPQNHWAVGQINLAYKLGLAQG VGNGKFDPNSELRYAQALAFVLRALGFKDLDWPYGYLAKAQDLGLVHGLNLAYNGVIKRG DLALILDRALEVPMVKYVDGKEVLGEPLISKVATKAEYTVIATNAQDRSVEEGKVAVLDK DGKLTTINAGLVDFSEYLGKKVIVYSERFGDPVYVAEGDNDVVSFTEGQDSVGTTVY*(297)KND DNKTAIKVDDNAYVLYNGYLTKVSKVTVKEGAEVTIINNNYLIVNGSYDNSTIVYNDVQS GDKYLNRDSNYELKGTVTVTGAVSKVTDIKANDYIYYGKQYDVNGNVVGTVIYVVRNQVT GTVTEKSVSGSTYKASIDNVSYTVADNNVWNQLEPGKKVTVILNKDNVIVGISSTTTTTA VNYAIFKEKSDPFTAWFAKVKLILPDAAEKVFDAVY*(516)SDVY*(520)DKVNLAEGTIVTYTVDANGK LNDIQRANDQPFSSASYKADAKVLTEGSTTYYITDNTVLLNNTSDGYKALKLTDLKDATN LNVKIVADNYNVAKVVVFNNASFVSTTTSTVY*(632)AYVTGTADVYVNGSTFTRLTVLENGQTK TYDANAQLATNYTHKAVVLTLTNAKIANIALPTVASGVKLTNIDQANLRITDTTNKGYLL DPNFIVVDTNGNLKGLSDITKDTGVNLYTNDVGKVFVIEIVQ
Sequence Around Glycosites (21 AA)EGQDSVGTTVYKNDDNKTAIK
DAAEKVFDAVYSDVYDKVNLA
KVFDAVYSDVYDKVNLAEGTI
SFVSTTTSTVYAYVTGTADVY
Glycosite Sequence Logoseqlogo
Glycosite Sequence Logo
Technique(s) used for Glycosylation DetectionDeglycosylation (trifluoromethanesulfonic acid), aberrant migration on SDS-PAGE.
Technique(s) used for Glycosylated Residue(s) DetectionProtease digestion and glycopeptide separation by molecular sieve chromatography and reversed-phase HPLC. Deglycosylated and undeglycosylated peptides were run on 2D PAGE and subjected to N-terminal sequencing post blotting.
Protein Glycosylation- ImplicationAn N terminal SLH sequence of A. kivui S layer glycoprotein is suggested to fold independently and present itself in a modular way that may serve as an anchor to the peptidoglycan. Glycosylated residues are surface exposed and implicated in protease K resistance.
Glycan Information
Glycan AnnotationCarbohydrate content 8% corresponding to 40 to 50 sugar molecules per protein monomer. Acidic, repetitive heterogenous glycans of varying length containing N-acetylglucosamine, 1,6 linked glucose, 1,4 linked galactosamine and/or unidentified sugar-related component.
Technique(s) used for Glycan IdentificationHydrazinolysis followed by amino-acid analysis as well as two-dimensional NMR spectroscopy
Protein Glycosylation linked (PGL) gene(s)
OST Gene Name
OST NCBI Gene ID
OST GenBank Gene Sequence
OST Protein Name
OST UniProtKB/ SwissProt ID
OST NCBI RefSeq
OST EMBL-CDS
OST UniProtKB Sequence
OST EC Number (BRENDA)
OST Genome Context
Characterized Accessory Gene(s)
PGL Additional LinksCAZy
Literatures
Reference(s)1) Lupas, A., Engelhardt, H., Peters, J., Santarius, U., Volker, S. and Baumeister, W. (1994) Domain structure of the Acetogenium kivui surface layer revealed by electron crystallography and sequence analysis. J Bacteriol, 176, 1224-1233. [PubMed: 8113161]
2) Peters, J., Rudolf, S., Oschkinat, H., Mengele, R., Sumper, M., Kellermann, J., Lottspeich, F. and Baumeister, W. (1992) Evidence for tyrosine-linked glycosaminoglycan in a bacterial surface protein. Biol Chem Hoppe Seyler, 373, 171-17
Additional CommentsA new recognition sequence for tyrosine linked glycosylation in A. kivui is suggested based on the fact that all glycosylated tyrosine in the sequence are preceded by valine.
Year of Identification1989
Year of Validation1992