ProGlyProt ID AC122 Organism Information Organism Name Sulfolobus acidocaldarius (DSM 639) Domain Archaea Classification Family: Sulfolobaceae Order: Sulfolobales Class: Thermoprotei or Crenarchaeota Division or phylum: "Crenarchaeota" Taxonomic ID (NCBI) 2285 Genome Sequence (s) GeneBank CP000077.1 EMBL CP000077 Gene Information Gene Name thpS (Saci_1714) NCBI Gene ID 3474479 GenBank Gene Sequence 3474479 Protein Information Protein Name Thermopsin UniProtKB/SwissProt ID P17118 NCBI RefSeq YP_256313.1 EMBL-CDS AAY81020.1 UniProtKB Sequence >sp|P17118|THPS_SULAC Thermopsin OS=Sulfolobus acidocaldarius GN=thpS PE=1 SV=1 MNFKSICLIILLSALIIPYIPQNIYFFPHRNTTGATISSGLYVNPYLYYTSPPAPAGIAS FGLYNYSGNVTPYVITTNEMLGYVNITSLLAYNREALRYGVDPYSATLQFNIVLSVNTSN GVYAYWLQDVGQFQTNKNSLTFIDNVWNLTGSLSTLSSSAITGNGQVASAGGGQTFYYDV GPSYTYSFPLSYIYIINMSYTSNAVYVWIGYEIIQIGQTEYGTVNYYDKITIYQPNIISA SLMINGNNYTPNGLYYDAELVWGGGGNGAPTSFNSLNCTLGLYYISNGSITPVPSLYTFG ADTAEAAYNVYTTMNNGVPIAYNGIENLTILTNNFSVILI Sequence length 340 AA Subcellular Location Secreted Function Thermostable acid protease. It has maximal proteolytic activity at pH 2 and 90 “C.Belongs to the peptidase A5 family. EC= 3.4.23.42. Protein Structure PDB ID Glycosylation Status Glycosylation Type N (Asn) linked Experimentally Validated Glycosite(s) in Full Length Protein (Signal peptide: 1-28, Propeptide: 28-41) N65, N69 Experimentally Validated Glycosite(s ) in Mature Protein N24, N28 Glycosite(s) Annotated Protein Sequence >sp|P17118|THPS_SULAC Thermopsin OS=Sulfolobus acidocaldarius GN=thpS PE=1 SV=1 MNFKSICLIILLSALIIPYIPQNIYFFPHRNTTGATISSGLYVNPYLYYTSPPAPAGIAS FGLYN*(65)YSGN*(69)VTPYVITTNEMLGYVNITSLLAYNREALRYGVDPYSATLQFNIVLSVNTSN GVYAYWLQDVGQFQTNKNSLTFIDNVWNLTGSLSTLSSSAITGNGQVASAGGGQTFYYDV GPSYTYSFPLSYIYIINMSYTSNAVYVWIGYEIIQIGQTEYGTVNYYDKITIYQPNIISA SLMINGNNYTPNGLYYDAELVWGGGGNGAPTSFNSLNCTLGLYYISNGSITPVPSLYTFG ADTAEAAYNVYTTMNNGVPIAYNGIENLTILTNNFSVILI Sequence Around Glycosites (21 AA) PAGIASFGLYNYSGNVTPYVI ASFGLYNYSGNVTPYVITTNE Glycosite Sequence Logo seqlogo Glycosite Sequence Logo Technique(s) used for Glycosylation Detection Aberrant SDS-PAGE migration. Technique(s) used for Glycosylated Residue(s) Detection Edman degradation Protein Glycosylation- Implication Glycan Information Glycan Annotation Technique(s) used for Glycan Identification Protein Glycosylation linked (PGL) gene(s) OST Gene Name Saci_1274 OST NCBI Gene ID 3473075 OST GenBank Gene Sequence 3473075 OST Protein Name Conserved membrane protein OST UniProtKB/ SwissProt ID Q4J9B4 OST NCBI RefSeq YP_255909.1 OST EMBL-CDS AAY80616.1 OST UniProtKB Sequence >tr|Q4J9B4|Q4J9B4_SULAC Conserved membrane protein OS=Sulfolobus acidocaldarius GN=Saci_1274 PE=4 SV=1 MQSTSILARIDKFKFLDAIIIGSLALFSILIRIISITAFPQTINGFDSWYLFYNALLIVK AGGNWYAVPPDVHAWFPWGYFIELENTIGLPFLVALFSVPFYSIFGQNIVYTLTLVSPIV LDGIGVVAAFLAVESITNSRVGGYIAAAITAFTPSLTYKNILGSLPKTSWGGVFVLFTIY FLSLAIKKKKPLYGIPAGIMIFLANITWGGYTYIDISLAIAAFLIVLFNKNDEISAKTLT ISGITAAFLTSLSPNTIGFMSEVAHGLALLIIPLFLYLDLYLRRVLPKDIVDSKNIVIGA AIILLVSLVVLGSVAFKVQLIPSRYYAIINPFFQFTVPIDRTVAEYIPQSIAAMIQDFGI GLFLSIIGIYFLLTRKQDMAGIWLVVLGAASIYGTSEQPYLFNYTIYIVAALAGVAVAEL FSRFMERKIRIAPILMLTLIGVALLADAGIAVEASYAPQALINSSTSYLTTNYAWISALD WINQNTPNNAFILSWWDYGYWIHAVGNRTVIDENNTLNGTQIKLMAEMFLNNESFAVNVL ENDFHLYPYGNPNYTRPVYIVAYDAVTEYIVNNQYPVWFIGYPTNFPGTFIGYTTSLGDI AKAIGAMTTIAGYNTNSYVNTTYINETASYAAQYNSQLASIIANSLPMAWTPKTYNSLIG SMFIEAIQSLNQGPVQAPFSISLSQLLQSSSTSLYNPNALLPRVNLMYFKPVYIALFPLS VTNALGGEAIVYIMVYIYQFVMPNVIIPPTISTA OST EC Number (BRENDA) 2.4.1.119 OST Genome Context Q4J9B4 Characterized Accessory Gene(s) PGL Additional Links CAZy Literatures Reference(s) 1) Lin, X. and Tang, J. (1990) Purification, characterization, and gene cloning of thermopsin, a thermostable acid protease from Sulfolobus acidocaldarius. J Biol Chem, 265, 1490-1495. [PubMed: 2104843] Additional Comments Year of Identification 1990 Year of Validation 1990