ProGlyProt ID AC121 Organism Information Organism Name Sulfolobus acidocaldarius (DSM 639) Domain Archaea Classification Family: Sulfolobaceae Order: Sulfolobales Class: Thermoprotei or Crenarchaeota Division or phylum: "Crenarchaeota" Taxonomic ID (NCBI) 2285 Genome Sequence (s) GeneBank CP000077.1 EMBL CP000077 Gene Information Gene Name slaA (Saci_2355) or slp1 NCBI Gene ID 3472789 GenBank Gene Sequence 3472789 Protein Information Protein Name SlaA (S- layer protein) UniProtKB/SwissProt ID Q4J6E5 NCBI RefSeq YP_256929.1 EMBL-CDS AAY81636.1 UniProtKB Sequence >tr|Q4J6E5|Q4J6E5_SULAC Conserved protein OS=Sulfolobus acidocaldarius GN=Saci_2355 PE=4 SV=1 MNKLVGLLVSSLFLASILIGIAPAITTTALTPPVSAGGIQAYLLTGSGAPASGLVLFVVN VSNIQVSSSNVTNVISTVVSNIQINAKTENAQTGATTGSVTVRFPTSGYNAYYDSVDKVV FVVVSFLYPYTTTSVNIPLSYLSKYLPGLLTAQPYDETGAQVTSVSSTPFGSLIDTSTGQ QILGTNPVLTSYNSYTTQANTNMQEGVVSGTLTSFTLGGQSFSGSTVPVILYAPFIFSNS PYQAGLYNPMQVNGNLGSLSSEAYYHPVIWGRALINTTLIDTYASGSVPFTFQLNYSVPG PLTINMAQLAWIASINNLPTSFTYLSYKFSNGYESFLGIISNSTQLTAGALTINPSGNFT INGKKFYVYLLVVGSTNSTTPVEYVTKLVVEYPSSTNFLPQGVTVTTSSNKYTLPVYEIG GPAGTTITLTGNWYSTPYTVQITVGSTPTLTNYVSQILLKAVAYEGINVSTTQSPYYSTA ILSTPPSEISITGSSTITAQGKLTATSASATVNLLTNATLTYENIPLTQYSFNGIIVTPG YAAINGTTAMAYVIGALYNKTSDYVLSFAGSQEPMQVMNNNLTEVTTLAPFGLTLLAPSV PATETGTSPLQLEFFTVPSTSYIALVDFGLWGNLTSVTVSAYDTVNNKLSVNLGYFYGIV IPPSISTAPYNYQNFICPNNYVTVTIYDPDAVLDPYPSGSFTTSSLPLKYGNMNITGAVI FPGSSVYNPSGVFGYSNFNKGAAVTTFTYTAQSGPFSPVALTGNTNYLSQYADNNPTDNY YFIQTVNGMPVLMGGLSIVASPVSASLPSSTSSPGFMYLLPSAAQVPSPLPGMATPNYNL NIYITYKIDGATVGNNMINGLYVASQNTLIYVVPNGSFVGSNIKLTYTTTDYAVLHYFYS TGQYKVFKTVSVPNVTANLYFPSSTTPLYQLSVPLYLSEPYYGSPLPTYIGLGTNGTSLW NSPNYVLFGVSAVQQYLGFIKSISVTLSNGTTVVIPLTTSNMQTLFPQLVGQELQACNGT FQFGISITGLEKLLNLNVQQLNNSILSVTYHDYVTGETLTATTKLVALSTLSLVAKGAGV VEFLLTAYPYTGNITFAPPWFIAENVVKQPFMTYSDLQFAKTNPSAILSLSTVNITVVGL GGKASVYYNSTSGQTVITNIYGQTVATLSGNVLPTLTELAAGNGTFTGSLQFTIVPNNTV VQIPSSLTKTSFAVYTNGSLAIVLNGKAYSLGPAGLFLLPFVTYTGSAIGANATAIITVS DGVGTSTTQVPITAENFTPIRLAPFQVPAQVPLPNAPKLKYEYNGSIVITPQQQVLKIYV TSILPYPQEFQIQAFVYEASQFNVHTGSPTAAPVYFSYSAVRAYPALGIGTSVPNLLVYV QLQGISNLPAGKYVIVLSAVPFAGGPVLSEYPAQLIFTNVTLTQ Sequence length 1424 AA Subcellular Location Surface Function The S-layer has to maintain the cell integrity and stabilize as well as to protect the cell against mechanical and osmotic stresses or extreme pH conditions. It is also predicted that the S-layer has to maintain or even determine the cell shape. Protein Structure PDB ID Glycosylation Status Glycosylation Type N (Asn) linked Experimentally Validated Glycosite(s) in Full Length Protein (Signal peptide : 1-29) N1042, N1093, N1134, N1197, N1217, N1252, N1276, N1304, N1419 Experimentally Validated Glycosite(s ) in Mature Protein N1013, N1064, N1105, N1168, N1188, N1223, N1247, N1275, N1390 Glycosite(s) Annotated Protein Sequence >tr|Q4J6E5|Q4J6E5_SULAC Conserved protein OS=Sulfolobus acidocaldarius GN=Saci_2355 PE=4 SV=1 MNKLVGLLVSSLFLASILIGIAPAITTTALTPPVSAGGIQAYLLTGSGAPASGLVLFVVN VSNIQVSSSNVTNVISTVVSNIQINAKTENAQTGATTGSVTVRFPTSGYNAYYDSVDKVV FVVVSFLYPYTTTSVNIPLSYLSKYLPGLLTAQPYDETGAQVTSVSSTPFGSLIDTSTGQ QILGTNPVLTSYNSYTTQANTNMQEGVVSGTLTSFTLGGQSFSGSTVPVILYAPFIFSNS PYQAGLYNPMQVNGNLGSLSSEAYYHPVIWGRALINTTLIDTYASGSVPFTFQLNYSVPG PLTINMAQLAWIASINNLPTSFTYLSYKFSNGYESFLGIISNSTQLTAGALTINPSGNFT INGKKFYVYLLVVGSTNSTTPVEYVTKLVVEYPSSTNFLPQGVTVTTSSNKYTLPVYEIG GPAGTTITLTGNWYSTPYTVQITVGSTPTLTNYVSQILLKAVAYEGINVSTTQSPYYSTA ILSTPPSEISITGSSTITAQGKLTATSASATVNLLTNATLTYENIPLTQYSFNGIIVTPG YAAINGTTAMAYVIGALYNKTSDYVLSFAGSQEPMQVMNNNLTEVTTLAPFGLTLLAPSV PATETGTSPLQLEFFTVPSTSYIALVDFGLWGNLTSVTVSAYDTVNNKLSVNLGYFYGIV IPPSISTAPYNYQNFICPNNYVTVTIYDPDAVLDPYPSGSFTTSSLPLKYGNMNITGAVI FPGSSVYNPSGVFGYSNFNKGAAVTTFTYTAQSGPFSPVALTGNTNYLSQYADNNPTDNY YFIQTVNGMPVLMGGLSIVASPVSASLPSSTSSPGFMYLLPSAAQVPSPLPGMATPNYNL NIYITYKIDGATVGNNMINGLYVASQNTLIYVVPNGSFVGSNIKLTYTTTDYAVLHYFYS TGQYKVFKTVSVPNVTANLYFPSSTTPLYQLSVPLYLSEPYYGSPLPTYIGLGTNGTSLW NSPNYVLFGVSAVQQYLGFIKSISVTLSNGTTVVIPLTTSNMQTLFPQLVGQELQACNGT FQFGISITGLEKLLNLNVQQLN*(1042)NSILSVTYHDYVTGETLTATTKLVALSTLSLVAKGAGV VEFLLTAYPYTGN*(1093)ITFAPPWFIAENVVKQPFMTYSDLQFAKTNPSAILSLSTVN*(1134)ITVVGL GGKASVYYNSTSGQTVITNIYGQTVATLSGNVLPTLTELAAGNGTFTGSLQFTIVPN*(1197)NTV VQIPSSLTKTSFAVYTN*(1217)GSLAIVLNGKAYSLGPAGLFLLPFVTYTGSAIGAN*(1252)ATAIITVS DGVGTSTTQVPITAEN*(1276)FTPIRLAPFQVPAQVPLPNAPKLKYEYN*(1304)GSIVITPQQQVLKIYV TSILPYPQEFQIQAFVYEASQFNVHTGSPTAAPVYFSYSAVRAYPALGIGTSVPNLLVYV QLQGISNLPAGKYVIVLSAVPFAGGPVLSEYPAQLIFTN*(1419)VTLTQ Sequence Around Glycosites (21 AA) KLLNLNVQQLNNSILSVTYHD FLLTAYPYTGNITFAPPWFIA PSAILSLSTVNITVVGLGGKA TGSLQFTIVPNNTVVQIPSSL LTKTSFAVYTNGSLAIVLNGK VTYTGSAIGANATAIITVSDG STTQVPITAENFTPIRLAPFQ PNAPKLKYEYNGSIVITPQQQ SEYPAQLIFTNVTLTQ Glycosite Sequence Logo seqlogo Glycosite Sequence Logo Technique(s) used for Glycosylation Detection Glycan identification by nano-LC-MS/MS Technique(s) used for Glycosylated Residue(s) Detection In-gel tryptic digestion and nano-LC-ES-MS/MS (nano-liquid chromatography-electrospray tandem mass spectrometry) Protein Glycosylation- Implication Glycosylation of S layer protein might contribute towards increased stability and half life of S layer structural proteins in extreme thermoacidic enviornments. Glycan Information Glycan Annotation Linkage: β-GlcNAc-Asn. N-linked hexasaccharide units - two residues each of Man and GlcNAc (one trisubstituted) and one residue each of Glc and 6-deoxy-6-sulfoglucose (6-sulfoquinovose, QuiS - a rare acidic sugar). β-D-Glcp-(1→4)-β-D-Quip(6--SO3-)-(1→3)-[α-D-Manp(1→4), α-D-Manp(1→6)] β-D-GlcpNAc-(1→4)-β-D-GlcpNAc-(1→Asn.... Microheterogeneity is observed as a family of glycans modify each of the glycosylation sites. The glycans are linked via a chitobiose core. Technique(s) used for Glycan Identification GC-MS of trimethylsilyl (TMS) methyl glycoside derivatives and nano-LC-ESMS analysis Protein Glycosylation linked (PGL) gene(s) OST Gene Name Saci_1274 OST NCBI Gene ID 3473075 OST GenBank Gene Sequence 3473075 OST Protein Name Conserved membrane protein OST UniProtKB/ SwissProt ID Q4J9B4 OST NCBI RefSeq YP_255909.1 OST EMBL-CDS AAY80616.1 OST UniProtKB Sequence >tr|Q4J9B4|Q4J9B4_SULAC Conserved membrane protein OS=Sulfolobus acidocaldarius GN=Saci_1274 PE=4 SV=1 MQSTSILARIDKFKFLDAIIIGSLALFSILIRIISITAFPQTINGFDSWYLFYNALLIVK AGGNWYAVPPDVHAWFPWGYFIELENTIGLPFLVALFSVPFYSIFGQNIVYTLTLVSPIV LDGIGVVAAFLAVESITNSRVGGYIAAAITAFTPSLTYKNILGSLPKTSWGGVFVLFTIY FLSLAIKKKKPLYGIPAGIMIFLANITWGGYTYIDISLAIAAFLIVLFNKNDEISAKTLT ISGITAAFLTSLSPNTIGFMSEVAHGLALLIIPLFLYLDLYLRRVLPKDIVDSKNIVIGA AIILLVSLVVLGSVAFKVQLIPSRYYAIINPFFQFTVPIDRTVAEYIPQSIAAMIQDFGI GLFLSIIGIYFLLTRKQDMAGIWLVVLGAASIYGTSEQPYLFNYTIYIVAALAGVAVAEL FSRFMERKIRIAPILMLTLIGVALLADAGIAVEASYAPQALINSSTSYLTTNYAWISALD WINQNTPNNAFILSWWDYGYWIHAVGNRTVIDENNTLNGTQIKLMAEMFLNNESFAVNVL ENDFHLYPYGNPNYTRPVYIVAYDAVTEYIVNNQYPVWFIGYPTNFPGTFIGYTTSLGDI AKAIGAMTTIAGYNTNSYVNTTYINETASYAAQYNSQLASIIANSLPMAWTPKTYNSLIG SMFIEAIQSLNQGPVQAPFSISLSQLLQSSSTSLYNPNALLPRVNLMYFKPVYIALFPLS VTNALGGEAIVYIMVYIYQFVMPNVIIPPTISTA OST EC Number (BRENDA) 2.4.1.119 OST Genome Context Q4J9B4 Characterized Accessory Gene(s) PGL Additional Links CAZy Literatures Reference(s) 1) Peyfoon, E., Meyer, B., Hitchen, P.G., Panico, M., Morris, H.R., Haslam, S.M., Albers, S.V. and Dell, A. (2010) The S-layer glycoprotein of the crenarchaeote Sulfolobus acidocaldarius is glycosylated at multiple sites with chitobiose-linked N-glycans. Archaea, 2010. [PubMed: 20936123] 2) Veith, A., Klingl, A., Zolghadr, B., Lauber, K., Mentele, R., Lottspeich, F., Rachel, R., Albers, S.V. and Kletzin, A. (2009) Acidianus, Sulfolobus and Metallosphaera surface layers: structure, compositi Additional Comments Similar to eukaryotes and unlike other archaea, the tribranched glycan is linked via chitobiose core (GlcNAcβ1-4GlcNAc) to the Asn in S. acidocaldarius glycoproteins. Hence sulfolobus has been suggested as closest eukaryotic model for N glycosylation studies among archaeal speceis. The glycosylation density at C terminus of the protein averages around 1 site per 30-40 residues. Similarity found in glycan sequence in SlaA and cytochrome b558/566 subunit A strengthen the existing und Year of Identification 2010 Year of Validation 2010