ProGlyProt IDAC119
Organism Information
Organism NameStaphylothermus mari
DomainArchaea
ClassificationFamily: Desulfurococcaceae
Order: Desulfurococcales
Class: Thermoprotei or Crenarchaeota
Division or phylum: "Crenarchaeota"
Taxonomic ID (NCBI)2280
Genome Sequence (s)
EMBLU57967
Gene Information
Gene NameSmar_1008
NCBI Gene ID
Protein Information
Protein NameTetrabrachion (S layer glycoprotein)
UniProtKB/SwissProt IDQ54436
NCBI RefSeq
EMBL-CDSAAC44118.1
UniProtKB Sequence>tr|Q54436|Q54436_STAMA Tetrabrachion OS=Staphylothermus marinus PE=1 SV=1 MNRVLAYSLLAIMTLSLLIIPAPGIAQRITVGVSVKAGTYNFYNITPTTQTVEVTDNGML RVIINRTEATELGTTIRLAFILDTDKYDPNVGGYFLNVSNIGVYAPSDPTQSPYGGVIDI TQNSTLTDGTQVVGNVTVVNGGNNVIILIDLSKLPDLQNVVYITNVYTETSTTANLTNTL LRVKAFDAASWDAVISGNQFKILYIPSLCKYVKINVIHSPAIVGTNVDVIVSFHKYFSLV QSIAGVSLDITVDNKTQLNMTNYYNATENYVLATFINGNLTVGGSEVFSSPTVTVINAST FKYSGQVKDYAPTVADTATPWVRTLNKFEVEFRHEIVNXTHDLIFYIHCDSDTVSYDTWP FLIVNASLDITTTEVAFNSTTINPGDIVNFTAHNVPLQYLTATNYGVLRFQLINPALVVY VPVSNMTLSANTTTGIINGSFVLPDAPYGGLDYLTYLVFNDGKFIANGYITVSPCIETYV LTNTSAYAEDAGSSYIGRFVPGYTSVPGDYIVIKGYGFALSNLTGFTVSINNTDVIILNA TYNASTGKIIILAKLLDTNGTPIPVGAGFIRVGQNGTTNIAYAPFNVTRNSGLEKVLFNP RWFYNGTYYIEHDKLGDPYLYFPVDYPLVNNTFTTEMWPFNTTIEVIGWPTNTFTLKAFN KEFNLSFNLLTLSLTNGYNMTNLYNLTIPFLPYGNYTLLEGTLLSVNNRTVFTVHMGINV DLDSCGNGTLSITVVGAAPNTEYNFTFGYQVHDLNYGITRYISPQWNGTWNISLVTDIYG TGSTSVPLITLYPTSYVINATWDVITWLRLSGSGTLDLLFSVDVSYNGFTDNLTTPITYV FGPSDTTPGSFNIYVNTTYNVSVVRVAVDYLPRTNVVISVPETVLPGDTITVQIFPHHNE VWGFIEPTALFDENQLLGWYLTVRLVDPLSNTVVERVAGYYAGNLIVEDVDGDGDNEVWF VVNLTAPLVLGVDKTYRVDVELFLAVLNPSSNITGVTAVDNECYVQLDLNGTIYWNGLGS GIMLGGDGQIVTVLGVLEGKLDTIKDGIAEINATVNDINTYLKVNVTDLLKTINNSVVMI KNDTATLIIGQAEIKAKLDDLLNLTSQVNDTVTMILACCNNASKVLNRMEGTLNSTYTGV LNVKSDLSTLIDTANNVVIPKFNELYDNVTVEINASRDLIIQKISSVNDSLTTIISAGFN DVEAMISNLNTTLLNRIDELEGTLLFYMTANEQRLEGIINETADDIVYRLTVIIDDRYES LKNLITLRADRLEMIINDNVSTILASIGNVNLTVFNKLNDLEIELGDVNATINAGIFQIQ SNLGNANQLILDTLTSSKVEILNAISSNASAISSEIHNAVNQLSTLVLQVNDTLTLKITG EADNILNFLSSLEGSMNTGFNNVTSTLSAVENNILGKITDTSNLLSSKIDNTLSTLQDLI TSTSNDLKNSISSAKNDIVSSLSSKVDSSTQTLSTKLDDLKSAQESNTNSINNNIMLFGA ASLILLIVTIGLVGYRLIARRRVG
Sequence length1524 AA
Subcellular LocationSurface (anchored in the membrane at one end)
FunctionStructural unit of S layer making it stable towards extremely harsh conditions. Encoded as a precursor with 26 AA signal peptide.
Protein Structure
PDB ID1FE6, 1YBK
Glycosylation Status
Glycosylation TypeN (Asn) linked (O Ser/Thr- linked residues not yet identified)
Experimentally Validated Glycosite(s) in Full Length Protein N44, N605, N641, N685, N708, N1279, N1402
Experimentally Validated Glycosite(s ) in Mature Protein N44, N605, N641, N685, N708, N1279, N1402
Glycosite(s) Annotated Protein Sequence>tr|Q54436|Q54436_STAMA Tetrabrachion OS=Staphylothermus marinus PE=1 SV=1 MNRVLAYSLLAIMTLSLLIIPAPGIAQRITVGVSVKAGTYNFYN*(44)ITPTTQTVEVTDNGML RVIINRTEATELGTTIRLAFILDTDKYDPNVGGYFLNVSNIGVYAPSDPTQSPYGGVIDI TQNSTLTDGTQVVGNVTVVNGGNNVIILIDLSKLPDLQNVVYITNVYTETSTTANLTNTL LRVKAFDAASWDAVISGNQFKILYIPSLCKYVKINVIHSPAIVGTNVDVIVSFHKYFSLV QSIAGVSLDITVDNKTQLNMTNYYNATENYVLATFINGNLTVGGSEVFSSPTVTVINAST FKYSGQVKDYAPTVADTATPWVRTLNKFEVEFRHEIVNXTHDLIFYIHCDSDTVSYDTWP FLIVNASLDITTTEVAFNSTTINPGDIVNFTAHNVPLQYLTATNYGVLRFQLINPALVVY VPVSNMTLSANTTTGIINGSFVLPDAPYGGLDYLTYLVFNDGKFIANGYITVSPCIETYV LTNTSAYAEDAGSSYIGRFVPGYTSVPGDYIVIKGYGFALSNLTGFTVSINNTDVIILNA TYNASTGKIIILAKLLDTNGTPIPVGAGFIRVGQNGTTNIAYAPFNVTRNSGLEKVLFNP RWFYN*(605)GTYYIEHDKLGDPYLYFPVDYPLVNNTFTTEMWPFN*(641)TTIEVIGWPTNTFTLKAFN KEFNLSFNLLTLSLTNGYNMTNLYN*(685)LTIPFLPYGNYTLLEGTLLSVNN*(708)RTVFTVHMGINV DLDSCGNGTLSITVVGAAPNTEYNFTFGYQVHDLNYGITRYISPQWNGTWNISLVTDIYG TGSTSVPLITLYPTSYVINATWDVITWLRLSGSGTLDLLFSVDVSYNGFTDNLTTPITYV FGPSDTTPGSFNIYVNTTYNVSVVRVAVDYLPRTNVVISVPETVLPGDTITVQIFPHHNE VWGFIEPTALFDENQLLGWYLTVRLVDPLSNTVVERVAGYYAGNLIVEDVDGDGDNEVWF VVNLTAPLVLGVDKTYRVDVELFLAVLNPSSNITGVTAVDNECYVQLDLNGTIYWNGLGS GIMLGGDGQIVTVLGVLEGKLDTIKDGIAEINATVNDINTYLKVNVTDLLKTINNSVVMI KNDTATLIIGQAEIKAKLDDLLNLTSQVNDTVTMILACCNNASKVLNRMEGTLNSTYTGV LNVKSDLSTLIDTANNVVIPKFNELYDNVTVEINASRDLIIQKISSVNDSLTTIISAGFN DVEAMISNLNTTLLNRIDELEGTLLFYMTANEQRLEGIINETADDIVYRLTVIIDDRYES LKNLITLRADRLEMIINDN*(1279)VSTILASIGNVNLTVFNKLNDLEIELGDVNATINAGIFQIQ SNLGNANQLILDTLTSSKVEILNAISSNASAISSEIHNAVNQLSTLVLQVNDTLTLKITG EADNILNFLSSLEGSMNTGFNN*(1402)VTSTLSAVENNILGKITDTSNLLSSKIDNTLSTLQDLI TSTSNDLKNSISSAKNDIVSSLSSKVDSSTQTLSTKLDDLKSAQESNTNSINNNIMLFGA ASLILLIVTIGLVGYRLIARRRVG
Sequence Around Glycosites (21 AA)SVKAGTYNFYNITPTTQTVEV
KVLFNPRWFYNGTYYIEHDKL
NTFTTEMWPFNTTIEVIGWPT
TNGYNMTNLYNLTIPFLPYGN
LLEGTLLSVNNRTVFTVHMGI
ADRLEMIINDNVSTILASIGN
LEGSMNTGFNNVTSTLSAVEN
Glycosite Sequence Logoseqlogo
Glycosite Sequence Logo
Technique(s) used for Glycosylation DetectionCarbohydrate analysis using trifluoroacetic acid and GC/MS (gas chromatography/mass spectrometry).
Technique(s) used for Glycosylated Residue(s) DetectionEdman degradation
Protein Glycosylation- Implication
Glycan Information
Glycan AnnotationCarbohydrates present: glucose, mannose, and glucosamine. Both N and O linked glycans present.
β-elimination revealed that at least 15 carbohydrate chains are bound to Ser or Thr. Two molecules of 150 KDa proteases are associated with glycosylated tetrabrachion polypeptides (85 KDa & 92KDa) and for this tetrabrachion-protease complex overall carbohydrate content is 38%.
Technique(s) used for Glycan Identification
Protein Glycosylation linked (PGL) gene(s)
OST Gene Name
OST NCBI Gene ID
OST GenBank Gene Sequence
OST Protein Name
OST UniProtKB/ SwissProt ID
OST NCBI RefSeq
OST EMBL-CDS
OST UniProtKB Sequence
OST EC Number (BRENDA)
OST Genome Context
Characterized Accessory Gene(s)Information currently not available with us.
PGL Additional LinksCAZy
Literatures
Reference(s)1) Ozbek, S., Muller, J.F., Figgemeier, E. and Stetefeld, J. (2005) Favourable mediation of crystal contacts by cocoamidopropylbetaine (CAPB). Acta Crystallogr D Biol Crystallogr, 61, 477-480. [PubMed: 15805603]
2) Stetefeld, J., Jenny, M., Schulthess, T., Landwehr, R., Engel, J. and Kammerer, R.A. (2000) Crystal structure of a naturally occurring parallel right-handed coiled coil tetramer. Nat Struct Biol, 7, 772-776. [PubMed: 10966648]
3) Peters, J., Baumeister, W. and Lupas, A. (199
Additional Comments
Year of Identification1995
Year of Validation1996