| ProGlyProt ID | AC119 |
| Organism Information |
| Organism Name | Staphylothermus mari |
| Domain | Archaea |
| Classification | Family: Desulfurococcaceae
Order: Desulfurococcales
Class: Thermoprotei or Crenarchaeota
Division or phylum: "Crenarchaeota" |
| Taxonomic ID (NCBI) | 2280 |
| Genome Sequence (s) |
| EMBL | U57967 |
| Gene Information |
| Gene Name | Smar_1008 |
| NCBI Gene ID | |
| Protein Information |
| Protein Name | Tetrabrachion (S layer glycoprotein) |
| UniProtKB/SwissProt ID | Q54436 |
| NCBI RefSeq | |
| EMBL-CDS | AAC44118.1 |
| UniProtKB Sequence | >tr|Q54436|Q54436_STAMA Tetrabrachion OS=Staphylothermus marinus PE=1 SV=1
MNRVLAYSLLAIMTLSLLIIPAPGIAQRITVGVSVKAGTYNFYNITPTTQTVEVTDNGML
RVIINRTEATELGTTIRLAFILDTDKYDPNVGGYFLNVSNIGVYAPSDPTQSPYGGVIDI
TQNSTLTDGTQVVGNVTVVNGGNNVIILIDLSKLPDLQNVVYITNVYTETSTTANLTNTL
LRVKAFDAASWDAVISGNQFKILYIPSLCKYVKINVIHSPAIVGTNVDVIVSFHKYFSLV
QSIAGVSLDITVDNKTQLNMTNYYNATENYVLATFINGNLTVGGSEVFSSPTVTVINAST
FKYSGQVKDYAPTVADTATPWVRTLNKFEVEFRHEIVNXTHDLIFYIHCDSDTVSYDTWP
FLIVNASLDITTTEVAFNSTTINPGDIVNFTAHNVPLQYLTATNYGVLRFQLINPALVVY
VPVSNMTLSANTTTGIINGSFVLPDAPYGGLDYLTYLVFNDGKFIANGYITVSPCIETYV
LTNTSAYAEDAGSSYIGRFVPGYTSVPGDYIVIKGYGFALSNLTGFTVSINNTDVIILNA
TYNASTGKIIILAKLLDTNGTPIPVGAGFIRVGQNGTTNIAYAPFNVTRNSGLEKVLFNP
RWFYNGTYYIEHDKLGDPYLYFPVDYPLVNNTFTTEMWPFNTTIEVIGWPTNTFTLKAFN
KEFNLSFNLLTLSLTNGYNMTNLYNLTIPFLPYGNYTLLEGTLLSVNNRTVFTVHMGINV
DLDSCGNGTLSITVVGAAPNTEYNFTFGYQVHDLNYGITRYISPQWNGTWNISLVTDIYG
TGSTSVPLITLYPTSYVINATWDVITWLRLSGSGTLDLLFSVDVSYNGFTDNLTTPITYV
FGPSDTTPGSFNIYVNTTYNVSVVRVAVDYLPRTNVVISVPETVLPGDTITVQIFPHHNE
VWGFIEPTALFDENQLLGWYLTVRLVDPLSNTVVERVAGYYAGNLIVEDVDGDGDNEVWF
VVNLTAPLVLGVDKTYRVDVELFLAVLNPSSNITGVTAVDNECYVQLDLNGTIYWNGLGS
GIMLGGDGQIVTVLGVLEGKLDTIKDGIAEINATVNDINTYLKVNVTDLLKTINNSVVMI
KNDTATLIIGQAEIKAKLDDLLNLTSQVNDTVTMILACCNNASKVLNRMEGTLNSTYTGV
LNVKSDLSTLIDTANNVVIPKFNELYDNVTVEINASRDLIIQKISSVNDSLTTIISAGFN
DVEAMISNLNTTLLNRIDELEGTLLFYMTANEQRLEGIINETADDIVYRLTVIIDDRYES
LKNLITLRADRLEMIINDNVSTILASIGNVNLTVFNKLNDLEIELGDVNATINAGIFQIQ
SNLGNANQLILDTLTSSKVEILNAISSNASAISSEIHNAVNQLSTLVLQVNDTLTLKITG
EADNILNFLSSLEGSMNTGFNNVTSTLSAVENNILGKITDTSNLLSSKIDNTLSTLQDLI
TSTSNDLKNSISSAKNDIVSSLSSKVDSSTQTLSTKLDDLKSAQESNTNSINNNIMLFGA
ASLILLIVTIGLVGYRLIARRRVG |
| Sequence length | 1524 AA |
| Subcellular Location | Surface (anchored in the membrane at one end) |
| Function | Structural unit of S layer making it stable towards extremely harsh conditions. Encoded as a precursor with 26 AA signal peptide. |
| Protein Structure |
| PDB ID | 1FE6, 1YBK |
| Glycosylation Status |
| Glycosylation Type | N (Asn) linked (O Ser/Thr- linked residues not yet identified) |
| Experimentally Validated Glycosite(s) in Full Length Protein | N44, N605, N641, N685, N708, N1279, N1402 |
| Experimentally Validated Glycosite(s ) in Mature Protein | N44, N605, N641, N685, N708, N1279, N1402 |
| Glycosite(s) Annotated Protein Sequence | >tr|Q54436|Q54436_STAMA Tetrabrachion OS=Staphylothermus marinus PE=1 SV=1
MNRVLAYSLLAIMTLSLLIIPAPGIAQRITVGVSVKAGTYNFYN*(44)ITPTTQTVEVTDNGML
RVIINRTEATELGTTIRLAFILDTDKYDPNVGGYFLNVSNIGVYAPSDPTQSPYGGVIDI
TQNSTLTDGTQVVGNVTVVNGGNNVIILIDLSKLPDLQNVVYITNVYTETSTTANLTNTL
LRVKAFDAASWDAVISGNQFKILYIPSLCKYVKINVIHSPAIVGTNVDVIVSFHKYFSLV
QSIAGVSLDITVDNKTQLNMTNYYNATENYVLATFINGNLTVGGSEVFSSPTVTVINAST
FKYSGQVKDYAPTVADTATPWVRTLNKFEVEFRHEIVNXTHDLIFYIHCDSDTVSYDTWP
FLIVNASLDITTTEVAFNSTTINPGDIVNFTAHNVPLQYLTATNYGVLRFQLINPALVVY
VPVSNMTLSANTTTGIINGSFVLPDAPYGGLDYLTYLVFNDGKFIANGYITVSPCIETYV
LTNTSAYAEDAGSSYIGRFVPGYTSVPGDYIVIKGYGFALSNLTGFTVSINNTDVIILNA
TYNASTGKIIILAKLLDTNGTPIPVGAGFIRVGQNGTTNIAYAPFNVTRNSGLEKVLFNP
RWFYN*(605)GTYYIEHDKLGDPYLYFPVDYPLVNNTFTTEMWPFN*(641)TTIEVIGWPTNTFTLKAFN
KEFNLSFNLLTLSLTNGYNMTNLYN*(685)LTIPFLPYGNYTLLEGTLLSVNN*(708)RTVFTVHMGINV
DLDSCGNGTLSITVVGAAPNTEYNFTFGYQVHDLNYGITRYISPQWNGTWNISLVTDIYG
TGSTSVPLITLYPTSYVINATWDVITWLRLSGSGTLDLLFSVDVSYNGFTDNLTTPITYV
FGPSDTTPGSFNIYVNTTYNVSVVRVAVDYLPRTNVVISVPETVLPGDTITVQIFPHHNE
VWGFIEPTALFDENQLLGWYLTVRLVDPLSNTVVERVAGYYAGNLIVEDVDGDGDNEVWF
VVNLTAPLVLGVDKTYRVDVELFLAVLNPSSNITGVTAVDNECYVQLDLNGTIYWNGLGS
GIMLGGDGQIVTVLGVLEGKLDTIKDGIAEINATVNDINTYLKVNVTDLLKTINNSVVMI
KNDTATLIIGQAEIKAKLDDLLNLTSQVNDTVTMILACCNNASKVLNRMEGTLNSTYTGV
LNVKSDLSTLIDTANNVVIPKFNELYDNVTVEINASRDLIIQKISSVNDSLTTIISAGFN
DVEAMISNLNTTLLNRIDELEGTLLFYMTANEQRLEGIINETADDIVYRLTVIIDDRYES
LKNLITLRADRLEMIINDN*(1279)VSTILASIGNVNLTVFNKLNDLEIELGDVNATINAGIFQIQ
SNLGNANQLILDTLTSSKVEILNAISSNASAISSEIHNAVNQLSTLVLQVNDTLTLKITG
EADNILNFLSSLEGSMNTGFNN*(1402)VTSTLSAVENNILGKITDTSNLLSSKIDNTLSTLQDLI
TSTSNDLKNSISSAKNDIVSSLSSKVDSSTQTLSTKLDDLKSAQESNTNSINNNIMLFGA
ASLILLIVTIGLVGYRLIARRRVG
|
| Sequence Around Glycosites (21 AA) | SVKAGTYNFYNITPTTQTVEV
KVLFNPRWFYNGTYYIEHDKL
NTFTTEMWPFNTTIEVIGWPT
TNGYNMTNLYNLTIPFLPYGN
LLEGTLLSVNNRTVFTVHMGI
ADRLEMIINDNVSTILASIGN
LEGSMNTGFNNVTSTLSAVEN
|
| Glycosite Sequence Logo | seqlogo |
| Glycosite Sequence Logo |  |
| Technique(s) used for Glycosylation Detection | Carbohydrate analysis using trifluoroacetic acid and GC/MS (gas chromatography/mass spectrometry). |
| Technique(s) used for Glycosylated Residue(s) Detection | Edman degradation |
| Protein Glycosylation- Implication | |
| Glycan Information |
| Glycan Annotation | Carbohydrates present: glucose, mannose, and glucosamine. Both N and O linked glycans present.
β-elimination revealed that at least 15 carbohydrate chains are bound to Ser or Thr. Two molecules of 150 KDa proteases are associated with glycosylated tetrabrachion polypeptides (85 KDa & 92KDa) and for this tetrabrachion-protease complex overall carbohydrate content is 38%. |
| Technique(s) used for Glycan Identification | |
| Protein Glycosylation linked (PGL) gene(s) |
| OST Gene Name | |
| OST NCBI Gene ID | |
| OST GenBank Gene Sequence | |
| OST Protein Name | |
| OST UniProtKB/ SwissProt ID | |
| OST NCBI RefSeq | |
| OST EMBL-CDS | |
| OST UniProtKB Sequence | |
| OST EC Number (BRENDA) | |
| OST Genome Context | |
| Characterized Accessory Gene(s) | Information currently not available with us. |
| PGL Additional Links | CAZy |
| Literatures |
| Reference(s) | 1) Ozbek, S., Muller, J.F., Figgemeier, E. and Stetefeld, J. (2005) Favourable mediation of crystal contacts by cocoamidopropylbetaine (CAPB). Acta Crystallogr D Biol Crystallogr, 61, 477-480. [PubMed: 15805603]
2) Stetefeld, J., Jenny, M., Schulthess, T., Landwehr, R., Engel, J. and Kammerer, R.A. (2000) Crystal structure of a naturally occurring parallel right-handed coiled coil tetramer. Nat Struct Biol, 7, 772-776. [PubMed: 10966648]
3) Peters, J., Baumeister, W. and Lupas, A. (199 |
| Additional Comments | |
| Year of Identification | 1995 |
| Year of Validation | 1996 |
