ProGlyProt ID AC118 Organism Information Organism Name Pyrococcus furiosus Domain Archaea Classification Family: Thermococcaceae Order: Thermococcales Class: Thermococci or Protoarchaea Division or phylum: "Euryarchaeota" Taxonomic ID (NCBI) 2261 Genome Sequence (s) GeneBank AE009950.1 EMBL AE009950 Gene Information Gene Name pls (PF0287) NCBI Gene ID 1468121 GenBank Gene Sequence 1468121 Protein Information Protein Name Pyrolysin UniProtKB/SwissProt ID P72186 NCBI RefSeq NP_578016.1 EMBL-CDS AAL80411.1 UniProtKB Sequence >sp|P72186|PLS_PYRFU Pyrolysin OS=Pyrococcus furiosus GN=pls PE=1 SV=2 MNKKGLTVLFIAIMLLSVVPVHFVSAGTPPVSSENSTTSILPNQQVVTKEVSQAALNAIM KGQPNMVLIIKTKEGKLEEAKTELEKLGAEILDENRVLNMLLVKIKPEKVKELNYISSLE KAWLNREVKLSPPIVEKDVKTKEPSLEPKMYNSTWVINALQFIQEFGYDGSGVVVAVLDT GVDPNHPFLSITPDGRRKIIEWKDFTDEGFVDTSFSFSKVVNGTLIINTTFQVASGLTLN ESTGLMEYVVKTVYVSNVTIGNITSANGIYHFGLLPERYFDLNFDGDQEDFYPVLLVNST GNGYDIAYVDTDLDYDFTDEVPLGQYNVTYDVAVFSYYYGPLNYVLAEIDPNGEYAVFGW DGHGHGTHVAGTVAGYDSNNDAWDWLSMYSGEWEVFSRLYGWDYTNVTTDTVQGVAPGAQ IMAIRVLRSDGRGSMWDIIEGMTYAATHGADVISMSLGGNAPYLDGTDPESVAVDELTEK YGVVFVIAAGNEGPGINIVGSPGVATKAITVGAAAVPINVGVYVSQALGYPDYYGFYYFP AYTNVRIAFFSSRGPRIDGEIKPNVVAPGYGIYSSLPMWIGGADFMSGTSMATPHVSGVV ALLISGAKAEGIYYNPDIIKKVLESGATWLEGDPYTGQKYTELDQGHGLVNVTKSWEILK AINGTTLPIVDHWADKSYSDFAEYLGVDVIRGLYARNSIPDIVEWHIKYVGDTEYRTFEI YATEPWIKPFVSGSVILENNTEFVLRVKYDVEGLEPGLYVGRIIIDDPTTPVIEDEILNT IVIPEKFTPENNYTLTWYDINGPEMVTHHFFTVPEGVDVLYAMTTYWDYGLYRPDGMFVF PYQLDYLPAAVSNPMPGNWELVWTGFNFAPLYESGFLVRIYGVEITPSVWYINRTYLDTN TEFSIEFNITNIYAPINATLIPIGLGTYNASVESVGDGEFFIKGIEVPEGTAELKIRIGN PSVPNSDLDLYLYDSKGNLVALDGNPTAEEEVVVEYPKPGVYSIVVHGYSVRDENGNPTT TTFDLVVQMTLDNGNIKLDKDSIILGSNESVVVTANITIDRDHPTGVYSGIIEIRDNEVY QDTNTSIAKIPITLVIDKADFAVGLTPAEGVLGEARNYTLIVKHALTLEPVPNATVIIGN YTYLTDENGTVTFTYAPTKLGSDEITVIVKKENFNTLEKTFQITVSEPEITEEDINEPKL AMSSPEANATIVSVEMESEGGVKKTVTVEITINGTANETATIVVPVPKKAENIEVSGDHV ISYSIEEGEYAKYVIITVKFASPVTVTVTYTIYAGPRVSILTLNFLGYSWYRLYSQKFDE LYQKALELGVDNETLALALSYHEKAKEYYEKALELSEGNIIQYLGDIRLLPPLRQAYINE MKAVKILEKAIEELEGEE Sequence length 1398 AA Subcellular Location Cell envelope associated Function Hyperthermostable serine protease. Has endopeptidase activity toward caseins, casein fragments including alpha-S1-casein and synthetic peptides. EC= 3.1.21.- Protein Structure PDB ID Glycosylation Status Glycosylation Type N (Asn) linked Experimentally Validated Glycosite(s) in Full Length Protein (Signal peptide: 1-26, propeptide: 26-149) N152 Experimentally Validated Glycosite(s ) in Mature Protein N3 Glycosite(s) Annotated Protein Sequence >sp|P72186|PLS_PYRFU Pyrolysin OS=Pyrococcus furiosus GN=pls PE=1 SV=2 MNKKGLTVLFIAIMLLSVVPVHFVSAGTPPVSSENSTTSILPNQQVVTKEVSQAALNAIM KGQPNMVLIIKTKEGKLEEAKTELEKLGAEILDENRVLNMLLVKIKPEKVKELNYISSLE KAWLNREVKLSPPIVEKDVKTKEPSLEPKMYN*(152)STWVINALQFIQEFGYDGSGVVVAVLDT GVDPNHPFLSITPDGRRKIIEWKDFTDEGFVDTSFSFSKVVNGTLIINTTFQVASGLTLN ESTGLMEYVVKTVYVSNVTIGNITSANGIYHFGLLPERYFDLNFDGDQEDFYPVLLVNST GNGYDIAYVDTDLDYDFTDEVPLGQYNVTYDVAVFSYYYGPLNYVLAEIDPNGEYAVFGW DGHGHGTHVAGTVAGYDSNNDAWDWLSMYSGEWEVFSRLYGWDYTNVTTDTVQGVAPGAQ IMAIRVLRSDGRGSMWDIIEGMTYAATHGADVISMSLGGNAPYLDGTDPESVAVDELTEK YGVVFVIAAGNEGPGINIVGSPGVATKAITVGAAAVPINVGVYVSQALGYPDYYGFYYFP AYTNVRIAFFSSRGPRIDGEIKPNVVAPGYGIYSSLPMWIGGADFMSGTSMATPHVSGVV ALLISGAKAEGIYYNPDIIKKVLESGATWLEGDPYTGQKYTELDQGHGLVNVTKSWEILK AINGTTLPIVDHWADKSYSDFAEYLGVDVIRGLYARNSIPDIVEWHIKYVGDTEYRTFEI YATEPWIKPFVSGSVILENNTEFVLRVKYDVEGLEPGLYVGRIIIDDPTTPVIEDEILNT IVIPEKFTPENNYTLTWYDINGPEMVTHHFFTVPEGVDVLYAMTTYWDYGLYRPDGMFVF PYQLDYLPAAVSNPMPGNWELVWTGFNFAPLYESGFLVRIYGVEITPSVWYINRTYLDTN TEFSIEFNITNIYAPINATLIPIGLGTYNASVESVGDGEFFIKGIEVPEGTAELKIRIGN PSVPNSDLDLYLYDSKGNLVALDGNPTAEEEVVVEYPKPGVYSIVVHGYSVRDENGNPTT TTFDLVVQMTLDNGNIKLDKDSIILGSNESVVVTANITIDRDHPTGVYSGIIEIRDNEVY QDTNTSIAKIPITLVIDKADFAVGLTPAEGVLGEARNYTLIVKHALTLEPVPNATVIIGN YTYLTDENGTVTFTYAPTKLGSDEITVIVKKENFNTLEKTFQITVSEPEITEEDINEPKL AMSSPEANATIVSVEMESEGGVKKTVTVEITINGTANETATIVVPVPKKAENIEVSGDHV ISYSIEEGEYAKYVIITVKFASPVTVTVTYTIYAGPRVSILTLNFLGYSWYRLYSQKFDE LYQKALELGVDNETLALALSYHEKAKEYYEKALELSEGNIIQYLGDIRLLPPLRQAYINE MKAVKILEKAIEELEGEE Sequence Around Glycosites (21 AA) KEPSLEPKMYNSTWVINALQF Glycosite Sequence Logo seqlogo Glycosite Sequence Logo Technique(s) used for Glycosylation Detection Periodic acid-Schiff (PAS) staining Technique(s) used for Glycosylated Residue(s) Detection Edman degradation Protein Glycosylation- Implication The post-translational modification may function in the thermostabilization of the enzyme. Glycan Information Glycan Annotation Technique(s) used for Glycan Identification Protein Glycosylation linked (PGL) gene(s) OST Gene Name aglB OST NCBI Gene ID 1467988 OST GenBank Gene Sequence 1467988 OST Protein Name AglB/STT3 OST UniProtKB/ SwissProt ID Q8U4D2 OST NCBI RefSeq NP_577885.1 OST EMBL-CDS AAL80280.1 OST UniProtKB Sequence >tr|Q8U4D2|Q8U4D2_PYRFU Oligosaccharyl transferase stt3 subunit related protein OS=Pyrococcus furiosus GN=PF0156 PE=1 SV=1 MVKTQIKEKKKDEKVTIPLPGKIKTVLAFLVVLAFAAYGFYIRHLTAGKYFSDPDTFYHF EIYKLVLKEGLPRYYPMADAPFGSLIGEPLGLYILPAIFYKIISIFGYNELEAFLLWPPF VGFLSVIGVYLLGRKVLNEWAGMWGAIILSVLTANFSRTFSGNARGDGPFMMLFTFSAVL MLYYLTEENKNKKIIWGTLFVLLAGISTAAWNGSPFGLMVLLGFASFQTIILFIFGKINE LREFIKEYYPAYLGILAISYLLTIPGIGKIGGFVRFAFEVFLGLVFLAIVMLYGGKYLNY SDKKHRFAVVAVIVIAGFAGAYIYVGPKLFTLMGGAYQSTQVYETVQELAKTDWGDVKVY YGVEKPNGIVFFLGLVGAMIVTARYLYKLFKDGRRPHEELFAITFYVMSIYLLWTAARFL FLASYAIALMSGVFAGYVLETVEKMKESIPIKAALGGVIAIMLLLIPLTHGPLLAQSAKS MRTTEIETSGWEDALKWLRENTPEYSTATSWWDYGYWIESSLLGQRRASADGGHARDRDH ILALFLARDGNISEVDFESWELNYFLVYLNDWAKFNAISYLGGAITRREYNGDESGRGAV TTLLPLPRYGEKYVNLYAKVIVDVSNSSVKVTVGDRECDPLMVTFTPSGKTIKGTGTCSD GNAFPYVLHLTPTIGVLAYYKVATANFIKLAFGVPASTIPGFSDKLFSNFEPVYESGNVI VYRFTPFGIYKIEENINGTWKQVYNLTPGKHELKLYISAFGRDIENATLYIYAINNEKII EKIKIAEISHMDYLNEYPIAVNVTLPNATSYRFVLVQKGPIGVLLDAPKVNGEIRSPTNI LREGESGEIELKVGVDKDYTADL OST EC Number (BRENDA) 2.4.1.119 OST Genome Context Q8U4D2 Characterized Accessory Gene(s) PGL Additional Links CAZy Literatures Reference(s) 1) Igura, M. and Kohda, D. (2011) Selective control of oligosaccharide transfer efficiency for the N-glycosylation sequon by a point mutation in oligosaccharyltransferase. J Biol Chem, 286, 13255-13260. [PubMed: 21357684] 2) Igura, M., Maita, N., Kamishikiryo, J., Yamada, M., Obita, T., Maenaka, K. and Kohda, D. (2008) Structure-guided identification of a new catalytic motif of oligosaccharyltransferase. EMBO J, 27, 234-243. [PubMed: 18046457] 3) de Vos, W.M., Voorhorst, W.G., Dijkgraa Additional Comments Year of Identification 1996 Year of Validation 1996