ProGlyProt IDAC117
Organism Information
Organism NameMethanothermus fervi
DomainArchaea
ClassificationFamily: Methanothermaceae
Order: Methanobacteriales
Class: Methanobacteria or Archaeobacteria
Division or phylum: "Euryarchaeota"
Taxonomic ID (NCBI)2180
Genome Sequence (s)
EMBLX58297
Gene Information
Gene NameslgA
NCBI Gene ID
Protein Information
Protein NameSlgA (cell surface glycoprotein)
UniProtKB/SwissProt IDP27373
NCBI RefSeq
EMBL-CDSCAA41230.1
UniProtKB Sequence>sp|P27373|CSG_METFE Cell surface glycoprotein OS=Methanothermus fervidus GN=slgA PE=1 SV=1 MRKFTLLMLLLIVISMSGIAGAAEVKNLNTSKTFTKIQEAIDDPSTTDGNIIIVGPGNYT ENILVNKSLTLKSNGSAIINAVSSEKSTITIKANNVWIEGFIIIGGKNGIYMENVTGCTI TNNTIQNAFVSGWEYYGGNGICLVNSTNNTITNNIIRNNTWNGINVCESKGNIIKNNTIM YSGGIGIYVWGFNKFEGNNIIENNRIINATYGGIYLFRPSNNKICRNYIANVSSGGGGMS GAICIDVSDYNIVKDNIGINCDGGLFTDGMIGNEITNNIFKNCKVAVSESTYGPASRNNK IYGNYFINYETAISDPKGELVDNIWNTTEGGNYWSNYTGNNTGDGTGNIPYYYDNKPLVV DLAIEDIAAKPSGIEVRVKNLGKADIKKIDPLTKLKIKISCDNDVYETFIDPLSAGESQI VRWDKIVPEGNHTIKAEIPYSAEGYLIGTNIRDADISNNVFSKIVQGFVQNKTFTITLTN LGKSTITIKYYISIYTNPVNGTKVSYRELTITLKPNETKTIELGKYPFKYAVSGTMIVKN PSRYRIPLNLRIKYEIEGLNPQMREISKYIAPRGEFRYIARYTGKEEGYADVW
Sequence length 593 AA
Subcellular LocationSurface
FunctionIn Archaea, which do not possess other cell wall components, the S-layer has to maintain the cell integrity and stabilize as well as to protect the cell against mechanical and osmotic stresses or extreme pH conditions. It is also predicted that the S-layer has to maintain or even determine the cell shape.
Protein Structure
PDB ID
Glycosylation Status
Glycosylation TypeN (Asn) linked
Experimentally Validated Glycosite(s) in Full Length Protein(Signal peptide: 1-22) N29 in case of precursor protein.
Experimentally Validated Glycosite(s ) in Mature ProteinN7
Glycosite(s) Annotated Protein Sequence>sp|P27373|CSG_METFE Cell surface glycoprotein OS=Methanothermus fervidus GN=slgA PE=1 SV=1 MRKFTLLMLLLIVISMSGIAGAAEVKNLN*(29)TSKTFTKIQEAIDDPSTTDGNIIIVGPGNYT ENILVNKSLTLKSNGSAIINAVSSEKSTITIKANNVWIEGFIIIGGKNGIYMENVTGCTI TNNTIQNAFVSGWEYYGGNGICLVNSTNNTITNNIIRNNTWNGINVCESKGNIIKNNTIM YSGGIGIYVWGFNKFEGNNIIENNRIINATYGGIYLFRPSNNKICRNYIANVSSGGGGMS GAICIDVSDYNIVKDNIGINCDGGLFTDGMIGNEITNNIFKNCKVAVSESTYGPASRNNK IYGNYFINYETAISDPKGELVDNIWNTTEGGNYWSNYTGNNTGDGTGNIPYYYDNKPLVV DLAIEDIAAKPSGIEVRVKNLGKADIKKIDPLTKLKIKISCDNDVYETFIDPLSAGESQI VRWDKIVPEGNHTIKAEIPYSAEGYLIGTNIRDADISNNVFSKIVQGFVQNKTFTITLTN LGKSTITIKYYISIYTNPVNGTKVSYRELTITLKPNETKTIELGKYPFKYAVSGTMIVKN PSRYRIPLNLRIKYEIEGLNPQMREISKYIAPRGEFRYIARYTGKEEGYADVW
Sequence Around Glycosites (21 AA)IAGAAEVKNLNTSKTFTKIQE
Glycosite Sequence Logoseqlogo
Glycosite Sequence Logo
Technique(s) used for Glycosylation DetectionCarbohydrate detection (TLC, thin layer chromatography)
Technique(s) used for Glycosylated Residue(s) DetectionEdman degradation
Protein Glycosylation- Implication
Glycan Information
Glycan AnnotationLinkage: β-GlcNAc-Asn.
N-linked heterosaccharide is 17 % of molecular weight of the protein. Composition of the hexasaccharide (1061.83 Da) is α-D-3-O-MetManp-(1→6)-α-D-3-O-MetManp-[(1→2)-α-D-Manp]3-(1→4)-D-GalNAc.
3-O-methylglucose, N-acetylglucosamine, and galactose are also found in lesser amounts. 3-O-methylmannose could be partly replaced by 3-O-methylglucose.
Technique(s) used for Glycan IdentificationMethylation analysis, plasma desorption mass spectrometry, and high field 1H, 13C NMR spectroscopy- COSY (correlated spectroscopy), 1D-, 2D-TOCSY (total correlation spectroscopy), and HMBC (heteronuclear multiple bond coherence), HMQC (heteronuclear multiple quantum correlation).
Protein Glycosylation linked (PGL) gene(s)
OST Gene NameMfer_0177
OST NCBI Gene ID9961890
OST GenBank Gene Sequence9961890
OST Protein NameOligosaccharyl transferase stt3 subunit
OST UniProtKB/ SwissProt IDE3GXE8
OST NCBI RefSeqYP_004003742.1
OST EMBL-CDSADP76980.1
OST UniProtKB Sequence>tr|E3GXE8|E3GXE8_METFE Oligosaccharyl transferase STT3 subunit OS=Methanothermus fervidus DSM 2088 GN=Mfer_0177 PE=4 SV=1 MKKKMLNTIKQIAIIAILFSLAFYIRSEAVNIGGVPPDQKNFYKDPSTGLPYFSEIDSYY NLRLTTNFLTKGMLGDTKVNGKDWDLHSYYPPGRPVDYPPMIVYVTSFLYKFVNIFKKVS LPVVAFWTGAFIGSLVVIPAYLFVRKITNDYGGITAALLVAFAPVYFAHTFAGLFRTAMF NVLFPILIMWFFAESIVAKTFKRRVIFAVATAISIILFSLSWVGYVFYLAVIFVVALVYF GLRYFIERGKVEYVSKLDWIKGQRELLALLIVAIICGGILTIGGGPSAIPNALSQLVGAV QMQALTQTSSYPNVYISVAELQVPPFVMPIPGKPGSVFLPNQMSAVGQIGGLGVFLLGVA GIIALLWKAGSIYRSEFSLTKDFLKTTPKIRAGKTSKFKKRKRRISEEVSRDIQIEHLFY AVLFTIWSIMAGYAVTKGIRFAEPFSIPMSLSAGIFVGFAADFVKKHVRAELTALTLAFI CGILAASPFAPTVQMKFLAGIISAILVYIVRKHSLRSVIIALLIVGVAATPSIYGAQQFA SQIVPSSNTGMYNATKWIKDHTTPGTVIMSWWDFGHFFTYEADRPVTFDGGSQNTPRAYW IGKALLTNNETLSAGILRMLASSGDKAYLTLDNYTHDTAKTVEILDKTLGVPKSEARNIM INEYKLTPQQADTILQYTHPDKKRPFVLVCSSDMIPKAAWWSYFGSWNFETKNGTHYFYH ISQQVSPPQKEKDKTVIYTINEVMQNQMICTKIEKKANEINATILMGTKGNLNEVKPHKL VIIEGNRVVKNEIVDKDSNYGLVVIGQGGSYVTIVMDKQLEDSVFTKLFILGGFNQTTFK YLHSEPGVSIWTAQ
OST EC Number (BRENDA)
OST Genome Context
Characterized Accessory Gene(s)
PGL Additional LinksCAZy
Literatures
Reference(s)1) Karcher, U., Schroder, H., Haslinger, E., Allmaier, G., Schreiner, R., Wieland, F., Haselbeck, A. and Konig, H. (1993) Primary structure of the heterosaccharide of the surface glycoprotein of Methanothermus fervidus. J Biol Chem, 268, 26821-26826. [PubMed: 8262914]
2) Kalmokoff, M.L., Koval, S.F. and Jarrell, K.F. (1992) Relatedness of the flagellins from methanogens. Arch Microbiol, 157, 481-487. [PubMed: 1503530]
3) Brockl, G., Behr, M., Fabry, S., Hensel, R., Kaudewitz, H., Biend
Additional Comments
Year of Identification1989
Year of Validation1991