ProGlyProt ID AC117 Organism Information Organism Name Methanothermus fervi Domain Archaea Classification Family: Methanothermaceae Order: Methanobacteriales Class: Methanobacteria or Archaeobacteria Division or phylum: "Euryarchaeota" Taxonomic ID (NCBI) 2180 Genome Sequence (s) EMBL X58297 Gene Information Gene Name slgA NCBI Gene ID Protein Information Protein Name SlgA (cell surface glycoprotein) UniProtKB/SwissProt ID P27373 NCBI RefSeq EMBL-CDS CAA41230.1 UniProtKB Sequence >sp|P27373|CSG_METFE Cell surface glycoprotein OS=Methanothermus fervidus GN=slgA PE=1 SV=1 MRKFTLLMLLLIVISMSGIAGAAEVKNLNTSKTFTKIQEAIDDPSTTDGNIIIVGPGNYT ENILVNKSLTLKSNGSAIINAVSSEKSTITIKANNVWIEGFIIIGGKNGIYMENVTGCTI TNNTIQNAFVSGWEYYGGNGICLVNSTNNTITNNIIRNNTWNGINVCESKGNIIKNNTIM YSGGIGIYVWGFNKFEGNNIIENNRIINATYGGIYLFRPSNNKICRNYIANVSSGGGGMS GAICIDVSDYNIVKDNIGINCDGGLFTDGMIGNEITNNIFKNCKVAVSESTYGPASRNNK IYGNYFINYETAISDPKGELVDNIWNTTEGGNYWSNYTGNNTGDGTGNIPYYYDNKPLVV DLAIEDIAAKPSGIEVRVKNLGKADIKKIDPLTKLKIKISCDNDVYETFIDPLSAGESQI VRWDKIVPEGNHTIKAEIPYSAEGYLIGTNIRDADISNNVFSKIVQGFVQNKTFTITLTN LGKSTITIKYYISIYTNPVNGTKVSYRELTITLKPNETKTIELGKYPFKYAVSGTMIVKN PSRYRIPLNLRIKYEIEGLNPQMREISKYIAPRGEFRYIARYTGKEEGYADVW Sequence length 593 AA Subcellular Location Surface Function In Archaea, which do not possess other cell wall components, the S-layer has to maintain the cell integrity and stabilize as well as to protect the cell against mechanical and osmotic stresses or extreme pH conditions. It is also predicted that the S-layer has to maintain or even determine the cell shape. Protein Structure PDB ID Glycosylation Status Glycosylation Type N (Asn) linked Experimentally Validated Glycosite(s) in Full Length Protein (Signal peptide: 1-22) N29 in case of precursor protein. Experimentally Validated Glycosite(s ) in Mature Protein N7 Glycosite(s) Annotated Protein Sequence >sp|P27373|CSG_METFE Cell surface glycoprotein OS=Methanothermus fervidus GN=slgA PE=1 SV=1 MRKFTLLMLLLIVISMSGIAGAAEVKNLN*(29)TSKTFTKIQEAIDDPSTTDGNIIIVGPGNYT ENILVNKSLTLKSNGSAIINAVSSEKSTITIKANNVWIEGFIIIGGKNGIYMENVTGCTI TNNTIQNAFVSGWEYYGGNGICLVNSTNNTITNNIIRNNTWNGINVCESKGNIIKNNTIM YSGGIGIYVWGFNKFEGNNIIENNRIINATYGGIYLFRPSNNKICRNYIANVSSGGGGMS GAICIDVSDYNIVKDNIGINCDGGLFTDGMIGNEITNNIFKNCKVAVSESTYGPASRNNK IYGNYFINYETAISDPKGELVDNIWNTTEGGNYWSNYTGNNTGDGTGNIPYYYDNKPLVV DLAIEDIAAKPSGIEVRVKNLGKADIKKIDPLTKLKIKISCDNDVYETFIDPLSAGESQI VRWDKIVPEGNHTIKAEIPYSAEGYLIGTNIRDADISNNVFSKIVQGFVQNKTFTITLTN LGKSTITIKYYISIYTNPVNGTKVSYRELTITLKPNETKTIELGKYPFKYAVSGTMIVKN PSRYRIPLNLRIKYEIEGLNPQMREISKYIAPRGEFRYIARYTGKEEGYADVW Sequence Around Glycosites (21 AA) IAGAAEVKNLNTSKTFTKIQE Glycosite Sequence Logo seqlogo Glycosite Sequence Logo Technique(s) used for Glycosylation Detection Carbohydrate detection (TLC, thin layer chromatography) Technique(s) used for Glycosylated Residue(s) Detection Edman degradation Protein Glycosylation- Implication Glycan Information Glycan Annotation Linkage: β-GlcNAc-Asn. N-linked heterosaccharide is 17 % of molecular weight of the protein. Composition of the hexasaccharide (1061.83 Da) is α-D-3-O-MetManp-(1→6)-α-D-3-O-MetManp-[(1→2)-α-D-Manp]3-(1→4)-D-GalNAc. 3-O-methylglucose, N-acetylglucosamine, and galactose are also found in lesser amounts. 3-O-methylmannose could be partly replaced by 3-O-methylglucose. Technique(s) used for Glycan Identification Methylation analysis, plasma desorption mass spectrometry, and high field 1H, 13C NMR spectroscopy- COSY (correlated spectroscopy), 1D-, 2D-TOCSY (total correlation spectroscopy), and HMBC (heteronuclear multiple bond coherence), HMQC (heteronuclear multiple quantum correlation). Protein Glycosylation linked (PGL) gene(s) OST Gene Name Mfer_0177 OST NCBI Gene ID 9961890 OST GenBank Gene Sequence 9961890 OST Protein Name Oligosaccharyl transferase stt3 subunit OST UniProtKB/ SwissProt ID E3GXE8 OST NCBI RefSeq YP_004003742.1 OST EMBL-CDS ADP76980.1 OST UniProtKB Sequence >tr|E3GXE8|E3GXE8_METFE Oligosaccharyl transferase STT3 subunit OS=Methanothermus fervidus DSM 2088 GN=Mfer_0177 PE=4 SV=1 MKKKMLNTIKQIAIIAILFSLAFYIRSEAVNIGGVPPDQKNFYKDPSTGLPYFSEIDSYY NLRLTTNFLTKGMLGDTKVNGKDWDLHSYYPPGRPVDYPPMIVYVTSFLYKFVNIFKKVS LPVVAFWTGAFIGSLVVIPAYLFVRKITNDYGGITAALLVAFAPVYFAHTFAGLFRTAMF NVLFPILIMWFFAESIVAKTFKRRVIFAVATAISIILFSLSWVGYVFYLAVIFVVALVYF GLRYFIERGKVEYVSKLDWIKGQRELLALLIVAIICGGILTIGGGPSAIPNALSQLVGAV QMQALTQTSSYPNVYISVAELQVPPFVMPIPGKPGSVFLPNQMSAVGQIGGLGVFLLGVA GIIALLWKAGSIYRSEFSLTKDFLKTTPKIRAGKTSKFKKRKRRISEEVSRDIQIEHLFY AVLFTIWSIMAGYAVTKGIRFAEPFSIPMSLSAGIFVGFAADFVKKHVRAELTALTLAFI CGILAASPFAPTVQMKFLAGIISAILVYIVRKHSLRSVIIALLIVGVAATPSIYGAQQFA SQIVPSSNTGMYNATKWIKDHTTPGTVIMSWWDFGHFFTYEADRPVTFDGGSQNTPRAYW IGKALLTNNETLSAGILRMLASSGDKAYLTLDNYTHDTAKTVEILDKTLGVPKSEARNIM INEYKLTPQQADTILQYTHPDKKRPFVLVCSSDMIPKAAWWSYFGSWNFETKNGTHYFYH ISQQVSPPQKEKDKTVIYTINEVMQNQMICTKIEKKANEINATILMGTKGNLNEVKPHKL VIIEGNRVVKNEIVDKDSNYGLVVIGQGGSYVTIVMDKQLEDSVFTKLFILGGFNQTTFK YLHSEPGVSIWTAQ OST EC Number (BRENDA) OST Genome Context Characterized Accessory Gene(s) PGL Additional Links CAZy Literatures Reference(s) 1) Karcher, U., Schroder, H., Haslinger, E., Allmaier, G., Schreiner, R., Wieland, F., Haselbeck, A. and Konig, H. (1993) Primary structure of the heterosaccharide of the surface glycoprotein of Methanothermus fervidus. J Biol Chem, 268, 26821-26826. [PubMed: 8262914] 2) Kalmokoff, M.L., Koval, S.F. and Jarrell, K.F. (1992) Relatedness of the flagellins from methanogens. Arch Microbiol, 157, 481-487. [PubMed: 1503530] 3) Brockl, G., Behr, M., Fabry, S., Hensel, R., Kaudewitz, H., Biend Additional Comments Year of Identification 1989 Year of Validation 1991