ProGlyProt ID AC113 Organism Information Organism Name Methanococcus voltae PS Domain Archaea Classification Family: Methanococcaceae Order: Methanococcales Class: Methanococci or Methanothermea Division or phylum: "Euryarchaeota" Taxonomic ID (NCBI) 2188 Genome Sequence (s) EMBL M72148 Gene Information Gene Name flaB2 NCBI Gene ID Protein Information Protein Name Fla B2 UniProtKB/SwissProt ID P27804 NCBI RefSeq EMBL-CDS AAA73075.1 UniProtKB Sequence >sp|P27804|FLAB2_METVO Flagellin B2 OS=Methanococcus voltae GN=flaB2 PE=1 SV=1 MKIKEFMSNKKGASGIGTLIVFIAMVLVAAVAASVLINTSGFLQQKASTTGKESTEQVAS GLQISQVMGMHNNSNINKTAIYISPNAGSSAIDLSQAVIMLSDGSNKRVYKYNESSYKDL TNGGDIFDNANVEWIKATATKFGIVVIQDADESCTAANPVINKGDLVAITLNTTSFSTTP RTSITGTVQPEFGAPGIISFTTPATYLNDSKVVQLQ Sequence length 216 AA Subcellular Location Surface associated Function Flagellin structural protein Protein Structure PDB ID Glycosylation Status Glycosylation Type N (Asn) linked Experimentally Validated Glycosite(s) in Full Length Protein (Propeptide: 1-12) N38, N72, N77, N113, N172, N208 Experimentally Validated Glycosite(s ) in Mature Protein N26, N60, N65, N101, N160, N196 Glycosite(s) Annotated Protein Sequence >sp|P27804|FLAB2_METVO Flagellin B2 OS=Methanococcus voltae GN=flaB2 PE=1 SV=1 MKIKEFMSNKKGASGIGTLIVFIAMVLVAAVAASVLIN*(38)TSGFLQQKASTTGKESTEQVAS GLQISQVMGMHN*(72)NSNIN*(77)KTAIYISPNAGSSAIDLSQAVIMLSDGSNKRVYKYN*(113)ESSYKDL TNGGDIFDNANVEWIKATATKFGIVVIQDADESCTAANPVINKGDLVAITLN*(172)TTSFSTTP RTSITGTVQPEFGAPGIISFTTPATYLN*(208)DSKVVQLQ Sequence Around Glycosites (21 AA) VAAVAASVLINTSGFLQQKAS LQISQVMGMHNNSNINKTAIY VMGMHNNSNINKTAIYISPNA DGSNKRVYKYNESSYKDLTNG NKGDLVAITLNTTSFSTTPRT ISFTTPATYLNDSKVVQLQ Glycosite Sequence Logo seqlogo Glycosite Sequence Logo Technique(s) used for Glycosylation Detection Mass shift detected on SDS-polyacrylamide gel and glycan identification by nano-LC-MS/MS Technique(s) used for Glycosylated Residue(s) Detection Nano-LC-MS/MS (nano-liquid chromatography-electrospray tandem mass spectrometry) and nano-ESI-feCID-MS/MS Protein Glycosylation- Implication The glycans on flagellin proteins are involved in the flagella assembly process. Glycan Information Glycan Annotation Linkage: β-GlcNAc-Asn. Trisaccharide (779 Da) composed of β-Manp NAcA6Thr-(1– 4)- β-Glcp NAc3NAcA-(1–3)-β-Glcp NAc. The sugars are mannuronic acid with the attached threonine, diacetylated glucuronic acid, N-acetylglucosamine. In a second version of this strain (M. voltae PS*), the glycan is modified with one additional residue (either 220 or 262 Da) linked to the terminal modified mannuronic acid. Technique(s) used for Glycan Identification NanoLC–MS/MS analysis and NMR spectroscopy- COSY (correlated spectroscopy), TOCSY (total correlation spectroscopy), NOESY (nuclear Overhauser effect spectroscopy) spectra, and 1H-13C HMBC (heteronuclear multiple bond coherence) spectra. Protein Glycosylation linked (PGL) gene(s) OST Gene Name aglB OST NCBI Gene ID OST GenBank Gene Sequence OST Protein Name AglB OST UniProtKB/ SwissProt ID Q2EMT4 OST NCBI RefSeq OST EMBL-CDS  ABD17750.1 OST UniProtKB Sequence >tr|Q2EMT4|Q2EMT4_METVO STT3 OS=Methanococcus voltae GN=MVO1749 PE=4 SV=1 MTENNEKVKNSDSANNQSSKNSKFNFNFEDKKVKCAKTILIIIFLAFLSFQMRAQTADMG FTTNEQYLDVFSDDNGRMYLTALDPYYYLRMSENYLENGHTGDTLKNIDGQQVPWDSYKY GPTGARATFNLLSVVTVWVYQVWHAMDSTVTLMNAAFWVPAILSMFLITPIFFTVRRITS SDIGGAVAAILASLSPSIFVKTVAGFSDTPILEILPLLFIVWFIIEAIHYSKEKNYKSLI YGLLATLMLALYPFMWSAWWYGYYIVIAFLVIYAIYKGISYNSIAKYTKSKNNNHKDKIE SEKLEMLNILKISGLFIIGGAVLITALYGVSTTMNALQAPLNYLGLDEVSSQTGWPNVLT TVSELDTASLDEIISSSLGSIHLFAIGLIGIFLSLFRKVLTPVKQISNGLAEKLDIKYAL LLIIWFAVTFLAASKGVRFVALMVPPLSIGVGIFVGFIEQFIKNNLDKKYEYVAYPTIAI IVLYALFTIYRADSADLVRMLLPSNYVPIAEGIMLASLAVLIIYKVAELIAESNKKLVMN KIFMILLAIGLITPTIATIVPFYSVPTYNDGWGESLEWINTQTPNNSVVTCWWDNGHIYT WKTDRMVTFDGSSQNTPRAYWVGRAFSTSNESLANGIFRMLASSGDKAYTTDSVLIKKTG SIKNTVDVLNEILPLTKSDAQKALKNSSYKFTDTEVSEILDATHPKVTNPDYLITYNRMT SIASVWSYFGNWDFNLPAGTSRSEREAGSFQGLQTYATNINDTLIVRSLIQQTAEYNIYT LIEVRNETLTGAMMAVTNDGQMQTQQLNMHKVKLMVNENGKSKMYNSLADPDGQLSLLIK VDKNSIIGTDGSNNPVYSSSSWMATANLEDSVYSKLHFFDGEGLDTIKLEKESLDPTANG VQPGFKVFSVD OST EC Number (BRENDA) OST Genome Context Characterized Accessory Gene(s) AglH, AglC, AglK, AglA glycosyltransferases are involved in the biosynthesis of the glycan. AglH is a GlcNAc-1-phosphate transferase transfering first sugar to dolichol pyrophosphate. AglC and AglK add the second sugar residue and AglA adds the third sugar to the glycan. Characterized by gene deletion studies PGL Additional Links CAZy Literatures Reference(s) 1) Chaban, B., Logan, S.M., Kelly, J.F. and Jarrell, K.F. (2009) AglC and AglK are involved in biosynthesis and attachment of diacetylated glucuronic acid to the N-glycan in Methanococcus voltae. J Bacteriol, 191, 187-195. [PubMed: 18978056] 2) Shams-Eldin, H., Chaban, B., Niehus, S., Schwarz, R.T. and Jarrell, K.F. (2008) Identification of the archaeal alg7 gene homolog (encoding N-acetylglucosamine-1-phosphate transferase) of the N-linked glycosylation system by cross-domain complementati Additional Comments Post translational modification was detected in the year 1999. It was confirmed as glycosylation (by identifying the glycans) in 2005. Year of Identification 2005 Year of Validation 2005