| ProGlyProt ID | AC111 |
| Organism Information |
| Organism Name | Methanococcus maripaludis S2 |
| Domain | Archaea |
| Classification | Family: Methanococcaceae
Order: Methanococcales
Class: Methanococci or Methanothermea
Division or phylum: "Euryarchaeota" |
| Taxonomic ID (NCBI) | 39152 |
| Genome Sequence (s) |
| GeneBank | BX950229.1 |
| EMBL | BX950229 |
| Gene Information |
| Gene Name | flaB3 (MMP1668) |
| NCBI Gene ID | 2761414 |
| GenBank Gene Sequence | 2761414 |
| Protein Information |
| Protein Name | FlaB3 |
| UniProtKB/SwissProt ID | Q6LWP1 |
| NCBI RefSeq | NP_988788.1 |
| EMBL-CDS | CAF31224.1 |
| UniProtKB Sequence | >tr|Q6LWP1|Q6LWP1_METMP Flagellin B3 OS=Methanococcus maripaludis GN=flaB3 PE=3 SV=1
MVKKFMKSKKGAVGIGTLIIFIAMVLVAAIAASVIINTAGKLQHKASTVGEESTEQVASG
IQVLKVIGHADTKTTIDKLAVMVAPNVGGEIDLSTTILTLGTGDAKYSLVYDSTQHNADV
KDDGSDSIFDETEWGSGSKYGLIVLQDSDNSTAEATHPTINYGDKVYITVTMDVNSTSKV
SGEVIPDYGASGIVEFRAPSVFTETVVTLQ
|
| Sequence length | 210 AA |
| Subcellular Location | Surface associated |
| Function | Flagellin |
| Protein Structure |
| PDB ID | |
| Glycosylation Status |
| Glycosylation Type | N (Asn) linked |
| Experimentally Validated Glycosite(s) in Full Length Protein | (Signal peptide: 1-11) N150, N175
|
| Experimentally Validated Glycosite(s ) in Mature Protein | N139, N164
|
| Glycosite(s) Annotated Protein Sequence | >tr|Q6LWP1|Q6LWP1_METMP Flagellin B3 OS=Methanococcus maripaludis GN=flaB3 PE=3 SV=1
MVKKFMKSKKGAVGIGTLIIFIAMVLVAAIAASVIINTAGKLQHKASTVGEESTEQVASG
IQVLKVIGHADTKTTIDKLAVMVAPNVGGEIDLSTTILTLGTGDAKYSLVYDSTQHNADV
KDDGSDSIFDETEWGSGSKYGLIVLQDSDN*(150)STAEATHPTINYGDKVYITVTMDVN*(175)STSKV
SGEVIPDYGASGIVEFRAPSVFTETVVTLQ
|
| Sequence Around Glycosites (21 AA) | YGLIVLQDSDNSTAEATHPTI
KVYITVTMDVNSTSKVSGEVI
|
| Glycosite Sequence Logo | seqlogo |
| Glycosite Sequence Logo |  |
| Technique(s) used for Glycosylation Detection | Aberrant SDS-PAGE migration and glycan detection by MS |
| Technique(s) used for Glycosylated Residue(s) Detection | NanoLC–MS/MS (nano-liquid chromatography-electrospray tandem mass spectrometry) |
| Protein Glycosylation- Implication | Glycan is necessary for proper flagellar filament assembly and function. A minimal portion of the glycan (at least two sugars) is required to facilitate flagellar assembly and the presence of each additional sugar results in increased motility, as measured on semi-swarm plates. |
| Glycan Information |
| Glycan Annotation | Linkage: β-GalNAc-Asn.
N-linked tetrasaccharide (1036.4 Da) composed of N-acetylgalactosamine, di-acetylated glucuronic acid, an acetylated and acetamidino-modified mannuronic acid linked to threonine, and a novel terminal sugar: Sug-4-β-ManNAc3NAmA6Thr-4-β-GlcNAc3NAcA-3-β-GalNAc-Asn where Sug is a novel monosaccharide unit, (5S)-2-acetamido-2,4-dideoxy-5-O-methyl-α-L-erythro-hexos-5-ulo-1,5-pyranose. |
| Technique(s) used for Glycan Identification | NanoLC–MS/MS analysis and 1H, 13C NMR spectroscopy |
| Protein Glycosylation linked (PGL) gene(s) |
| OST Gene Name | aglB |
| OST NCBI Gene ID | 2761329 |
| OST GenBank Gene Sequence | 2761329 |
| OST Protein Name | AglB/STT3 subunit |
| OST UniProtKB/ SwissProt ID | Q6LXC8 |
| OST NCBI RefSeq | NP_988544.1 |
| OST EMBL-CDS | CAF30980.1 |
| OST UniProtKB Sequence | >tr|Q6LXC8|Q6LXC8_METMP Oligosaccharyl transferase, STT3 subunit OS=Methanococcus maripaludis GN=MMP1424 PE=4 SV=1
MGEFLNKVSDFFKKNEKIKIILILLFIGMMSFQIRAQTADMAFTDNSYLQDMFSDDNGRM
YLTALDPYYYLRMTENYVNNDYSNVGETTVGIDGENIPYDTIQYAPPGREAGLVSALSIA
TVLVYSVWNSIDSTVTIMNAAFWVPAIMSIFLGIPVFFIVRRNTASNIGGLVGALLLISS
PSLLYKTSAGFSDTPIFEILPLLFIVWMIMEAIHEQENSKKSGIFGGIAAILIGLYPMMW
SGWWYAFDITAGFLVLYTAYEYLTKSKNLKNVITTSLITLVGGAILVSLSTGLSGFINWI
LSPIGFTVINEATKITGWPNVYMTVSELAIPTVTDIIENSVGNIWLLIAGISGILLSFVS
FKHDKQKIDIKYALYLTLWLIATVYAATKGIRFVALMTPALAIGIGIFAGQIENIIKRYE
KKVEYILYPVIGILSVITLIKYGGELFNILVPTTYVPIAVYLSIIALLVLAVYKIIDIIS
EKEQAVKKVFGILLAFMLVFPSMAAAVPFYTAPTMNNGWMDSLSWIKSETPENSVVTCWW
DNGHIYTWATRKMVTFDGGSQNTPRAYWVGHAFSTSDENLSVGILRMLATSGDSAYDDDS
ILIKKTGSIKDTVDILNKILPLTRTEAKASLVNNYDLTDAEAEEVLDLTHPKVTNPDYLI
TYNRMTSIASVWSMFGNWNFSLPASTENSDREMGYYQQLGGSAQDINGTTVVYIPLQETD
SYRVINILEITDSEIKSANAVIDSNNQTSMQSPNFHKLILKVNGNVYEQETNENGDYSEI
VRLEKLSDGTYQVYAWVSSKNLEDSIYTKLHFLDGYGLEKISLEKESVDPTSYGIQPGFK
VYSVDYGTDYLN
|
| OST EC Number (BRENDA) | 2.4.1.119 |
| OST Genome Context | Q6LXC8 |
| Characterized Accessory Gene(s) | AglO, AglA, AglL are glycosyltransferases responsible for tetrasaccharide assembly. AglO adds the second sugar, AglA adds the third sugar, and AglL transfers the last sugar. MMP0350 (acetyltransferase-second sugar ), MMP1081 (Acetamidino transfer-third sugar), MMP1085 (Methyltransferase-fourth sugar). MMP1081 and MMP1085 are unpublished results as referred in ref. 4.
Characterized by gene deletion studies. |
| PGL Additional Links | CAZy |
| Literatures |
| Reference(s) | 1) Jarrell, K.F., Jones, G.M. and Nair, D.B. Biosynthesis and role of N-linked glycosylation in cell surface structures of archaea with a focus on flagella and s layers. Int J Microbiol, 2010, 470138. [PubMed: 20721273]
2) Kelly, J., Logan, S.M., Jarrell, K.F., VanDyke, D.J. and Vinogradov, E. (2009) A novel N-linked flagellar glycan from Methanococcus maripaludis. Carbohydr Res, 344, 648-653. [PubMed: 19203750]
3) VanDyke, D.J., Wu, J., Logan, S.M., Kelly, J.F., Mizuno, S., Aizawa, S |
| Additional Comments | Sug, a novel monosaccharide unit, (5S)-2-acetamido-2,4-dideoxy-5-O-methyl-α-L-erythro-hexos-5-ulo-1,5-pyranose is the first example of a naturally occuring diglycoside of an aldulose. |
| Year of Identification | 2008 |
| Year of Validation | 2009 |
