ProGlyProt ID AC107 Organism Information Organism Name Halobacterium salinarum (halobium) R1M1/NRC-1 Domain Archaea Classification Family: Halobacteriaceae Order: Halobacteriales Class: Halobacteria or Halomebacteria Division or phylum: "Euryarchaeota" Taxonomic ID (NCBI) 64091 Genome Sequence (s) GeneBank AE004437.1 EMBL AE004437 Gene Information Gene Name flaB3 (VNG_0962G) NCBI Gene ID 1447687 GenBank Gene Sequence 1447687 Protein Information Protein Name Flagellin B3 UniProtKB/SwissProt ID P13078 NCBI RefSeq NP_279905.1 EMBL-CDS AAG19385.1 UniProtKB Sequence >sp|P13078|FLAB3_HALSA Flagellin B3 OS=Halobacterium salinarium (strain ATCC 700922 / JCM 11081 / NRC-1) GN=flaB3 PE=3 SV=1 MFEFITDEDERGQVGIGTLIVFIAMVLVAAIAAGVLINTAGYLQSKGSATGEEASAQVSN RINIVSAYGNVNSEKVDYVNLTVRQAAGADNINLTKSTIQWIGPDKATTLTYSSNSPSSL GENFTTESIKGNNADVLVEQSDRIKVIMYASGVSSTLGSGEEVQLTVTTQYGSKTTYWAH VPESLKDKNAVKL Sequence length 193 AA Subcellular Location Flagellum Function Flagellin is the structural subunit of the flagellar filaments. Protein Structure PDB ID Glycosylation Status Glycosylation Type N (Asn) linked Experimentally Validated Glycosite(s) in Full Length Protein N91 Experimentally Validated Glycosite(s ) in Mature Protein N91 Glycosite(s) Annotated Protein Sequence >sp|P13078|FLAB3_HALSA Flagellin B3 OS=Halobacterium salinarium (strain ATCC 700922 / JCM 11081 / NRC-1) GN=flaB3 PE=3 SV=1 MFEFITDEDERGQVGIGTLIVFIAMVLVAAIAAGVLINTAGYLQSKGSATGEEASAQVSN RINIVSAYGNVNSEKVDYVNLTVRQAAGADN*(91)INLTKSTIQWIGPDKATTLTYSSNSPSSL GENFTTESIKGNNADVLVEQSDRIKVIMYASGVSSTLGSGEEVQLTVTTQYGSKTTYWAH VPESLKDKNAVKL Sequence Around Glycosites (21 AA) LTVRQAAGADNINLTKSTIQW Glycosite Sequence Logo seqlogo Glycosite Sequence Logo Technique(s) used for Glycosylation Detection Mass shift on SDS-PAGE after deglycosylation with anhydrous hydrogen fluoride. Technique(s) used for Glycosylated Residue(s) Detection Glycopeptide sequencing Protein Glycosylation- Implication Glycan Information Glycan Annotation Linkage: Glc-Asn. Sulfated oligosaccharides that resemble those of the cell-suface glycoprotein in the bacterium are present. Glucose and glucuronic acid are the constituents of the glycans which are of the type GlcA-(1→4)-GlcA-(1→4)-GlcA-(1→4)-Glc. Technique(s) used for Glycan Identification GLC-MS (gas liquid chromatography-mass spectrometry) after perfluoropropionylation. Protein Glycosylation linked (PGL) gene(s) OST Gene Name OST NCBI Gene ID OST GenBank Gene Sequence OST Protein Name OST UniProtKB/ SwissProt ID OST NCBI RefSeq OST EMBL-CDS OST UniProtKB Sequence OST EC Number (BRENDA) OST Genome Context Characterized Accessory Gene(s) PGL Additional Links CAZy Literatures Reference(s) 1) Cohen-Krausz, S. and Trachtenberg, S. (2002) The structure of the archeabacterial flagellar filament of the extreme halophile Halobacterium salinarum R1M1 and its relation to eubacterial flagellar filaments and type IV pili. J Mol Biol, 321, 383-395. [PubMed: 12162953] 2) Gerl, L., Deutzmann, R. and Sumper, M. (1989) Halobacterial flagellins are encoded by a multigene family. Identification of all five gene products. FEBS Lett, 244, 137-140. [PubMed: 2924901] 3) Gerl, L. and Sumper, M Additional Comments Removal of Mg++ ions in the growth medium inhibits glycosylation of proteins in vivo. This leads to the reduction in the molecular masses of newly synthesized flagellins. There might be another modification of the flagellins that is resistant to HF treatment. Halobacterial glycoproteins including flagellins are glycosylated at the extracellular surface of the cell membrane. Year of Identification 1985 Year of Validation 1988