ProGlyProt IDAC107
Organism Information
Organism NameHalobacterium salinarum (halobium) R1M1/NRC-1
DomainArchaea
ClassificationFamily: Halobacteriaceae
Order: Halobacteriales
Class: Halobacteria or Halomebacteria
Division or phylum: "Euryarchaeota"
Taxonomic ID (NCBI)64091
Genome Sequence (s)
GeneBankAE004437.1
EMBLAE004437
Gene Information
Gene NameflaB3 (VNG_0962G)
NCBI Gene ID1447687
GenBank Gene Sequence1447687
Protein Information
Protein NameFlagellin B3
UniProtKB/SwissProt IDP13078
NCBI RefSeqNP_279905.1
EMBL-CDSAAG19385.1
UniProtKB Sequence>sp|P13078|FLAB3_HALSA Flagellin B3 OS=Halobacterium salinarium (strain ATCC 700922 / JCM 11081 / NRC-1) GN=flaB3 PE=3 SV=1 MFEFITDEDERGQVGIGTLIVFIAMVLVAAIAAGVLINTAGYLQSKGSATGEEASAQVSN RINIVSAYGNVNSEKVDYVNLTVRQAAGADNINLTKSTIQWIGPDKATTLTYSSNSPSSL GENFTTESIKGNNADVLVEQSDRIKVIMYASGVSSTLGSGEEVQLTVTTQYGSKTTYWAH VPESLKDKNAVKL
Sequence length193 AA
Subcellular LocationFlagellum
FunctionFlagellin is the structural subunit of the flagellar filaments.
Protein Structure
PDB ID
Glycosylation Status
Glycosylation TypeN (Asn) linked
Experimentally Validated Glycosite(s) in Full Length ProteinN91
Experimentally Validated Glycosite(s ) in Mature ProteinN91
Glycosite(s) Annotated Protein Sequence>sp|P13078|FLAB3_HALSA Flagellin B3 OS=Halobacterium salinarium (strain ATCC 700922 / JCM 11081 / NRC-1) GN=flaB3 PE=3 SV=1 MFEFITDEDERGQVGIGTLIVFIAMVLVAAIAAGVLINTAGYLQSKGSATGEEASAQVSN RINIVSAYGNVNSEKVDYVNLTVRQAAGADN*(91)INLTKSTIQWIGPDKATTLTYSSNSPSSL GENFTTESIKGNNADVLVEQSDRIKVIMYASGVSSTLGSGEEVQLTVTTQYGSKTTYWAH VPESLKDKNAVKL
Sequence Around Glycosites (21 AA)LTVRQAAGADNINLTKSTIQW
Glycosite Sequence Logoseqlogo
Glycosite Sequence Logo
Technique(s) used for Glycosylation DetectionMass shift on SDS-PAGE after deglycosylation with anhydrous hydrogen fluoride.
Technique(s) used for Glycosylated Residue(s) DetectionGlycopeptide sequencing
Protein Glycosylation- Implication
Glycan Information
Glycan AnnotationLinkage: Glc-Asn.
Sulfated oligosaccharides that resemble those of the cell-suface glycoprotein in the bacterium are present. Glucose and glucuronic acid are the constituents of the glycans which are of the type GlcA-(1→4)-GlcA-(1→4)-GlcA-(1→4)-Glc.
Technique(s) used for Glycan IdentificationGLC-MS (gas liquid chromatography-mass spectrometry) after perfluoropropionylation.
Protein Glycosylation linked (PGL) gene(s)
OST Gene Name
OST NCBI Gene ID
OST GenBank Gene Sequence
OST Protein Name
OST UniProtKB/ SwissProt ID
OST NCBI RefSeq
OST EMBL-CDS
OST UniProtKB Sequence
OST EC Number (BRENDA)
OST Genome Context
Characterized Accessory Gene(s)
PGL Additional LinksCAZy
Literatures
Reference(s)1) Cohen-Krausz, S. and Trachtenberg, S. (2002) The structure of the archeabacterial flagellar filament of the extreme halophile Halobacterium salinarum R1M1 and its relation to eubacterial flagellar filaments and type IV pili. J Mol Biol, 321, 383-395. [PubMed: 12162953]
2) Gerl, L., Deutzmann, R. and Sumper, M. (1989) Halobacterial flagellins are encoded by a multigene family. Identification of all five gene products. FEBS Lett, 244, 137-140. [PubMed: 2924901]
3) Gerl, L. and Sumper, M
Additional CommentsRemoval of Mg++ ions in the growth medium inhibits glycosylation of proteins in vivo. This leads to the reduction in the molecular masses of newly synthesized flagellins. There might be another modification of the flagellins that is resistant to HF treatment. Halobacterial glycoproteins including flagellins are glycosylated at the extracellular surface of the cell membrane.
Year of Identification1985
Year of Validation1988