ProGlyProt IDAC101
Organism Information
Organism NameHalobacterium salinarum (halobium) R1M1/NRC-1
DomainArchaea
ClassificationFamily: Halobacteriaceae
Order: Halobacteriales
Class: Halobacteria or Halomebacteria
Division or phylum: "Euryarchaeota"
Taxonomic ID (NCBI)64091
Genome Sequence (s)
GeneBankAE004437.1
EMBLAE004437
Gene Information
Gene Namecsg (VNG_2679G)
NCBI Gene ID1449008
GenBank Gene Sequence1449008
Protein Information
Protein NameS layer glycopeptide
UniProtKB/SwissProt IDQ9HM69
NCBI RefSeqNP_281222.1
EMBL-CDSAAG20702.1
UniProtKB Sequence>sp|Q9HM69|CSG_HALSA Cell surface glycoprotein OS=Halobacterium salinarium GN=csg PE=1 SV=2 MTDTTGKLRAVLLTALMVGSVIGAGVAFTGGAAAANASDLNDYQRFNENTNYTYSTASED GKTEGSVASGATIFQGEEDVTFRKLDNEKEVSPATLSRTGGSDEGVPLQMPIPEDQSTGS YDSNGPDNDEADFGVTVQSPSVTMLEVRNNADNDVTGGVLNTQQDESSIAVDYNYYAAED LELTVEDEDGLDVTDEILAADQSGGAYEDGTGNNGPNTLRFDIDPNNVDAGDYTVSVEGV EDLDFGDATESASVTISSSNKASLNLAEDEVVQGANLKYTIENSPEGNYHAVTIDSSDFR DSSSGADAAKVMRSVGDTVDTGLVVDNDSTTEIVDDYENTSISDVDYAYAIVEIDDGNGV GSIETQYLDDSSADIDLYPASDTEDAPDYVNSNEELTNGSALDGVSTDDDTDFDVTQGDI TLDNPTGAYVVGSEVDINGTANEGTDDVVLYARDNNDFELVTVDGEKSIEVDSDDTFEEE DITLSDGDKGGDDILGLPGTYRLGIIAKSDAVNSSGGVKDNIDTSDFNQGVSSTSSIRVT DTELTASFETYNGQVADDDNQIDVEGTAPGKDNVAAIIIGSRGKVKFQSISVDSDDTFDE EDIDISELRQGSASAHILSSGRDGKFGEDTANSISDLEDEVGNYTSGSPTGDQIRDRILS NTVDDTASDDLIVTQQFRLVDGLTTIEATEGGEAGGSLTVMGTTNRKADDNTITVELLQG DASIEINSTDEWNSDGQWSVDVPLSNVEPGNYTVEADDGDNTDRQNVEIVEELEEPDQTT VDQPENNQTMTTTMTETTTETTTEMTTTQENTTENGSEGTSDGESGGSIPGFGVGVALVA VLGAALLALRQN
Sequence length852 AA
Subcellular LocationSurface
Function
Protein Structure
PDB ID
Glycosylation Status
Glycosylation TypeN (Asn) linked
Experimentally Validated Glycosite(s) in Full Length ProteinN2 [Replacement of the Ser residue at the consensus sequence by Val, Leu, and Asn, did not abolish glycosylation of Asn-2. However, replacing Ser-481 in the sequence Asn-Ser-Ser for Val prevented glycosylation of Asn-479.]
Experimentally Validated Glycosite(s ) in Mature ProteinN2
Glycosite(s) Annotated Protein SequenceAN*(2)AVDLNDYQR
(csg mutants with Val, Leu or Asn at 4th position were created)
Sequence Around Glycosites (21 AA)
Glycosite Sequence Logoseqlogo
Glycosite Sequence Logo
Technique(s) used for Glycosylation DetectionEdman degradation using an automated gas phase sequencer.
Technique(s) used for Glycosylated Residue(s) DetectionEdman degradation using gas phase sequencing (known residues are mutated).
Protein Glycosylation- Implication
Glycan Information
Glycan AnnotationSame sugar pattern like that of the wild-type is present. Galacturonic acid, 3-O-methyl-galacturonic acid, galactose, N-acetylglucosamine, and N-acetylgalactosamine have been observed.
Technique(s) used for Glycan IdentificationGas chromatographic analysis of pentafluoropropionyl-methyl-glycosides.
Protein Glycosylation linked (PGL) gene(s)
OST Gene NameaglB/stt3
OST NCBI Gene ID5953829
OST GenBank Gene Sequence5953829
OST Protein NameAglB/STT3 subunit
OST UniProtKB/ SwissProt IDB0R4T2
OST NCBI RefSeqYP_001689095.1
OST EMBL-CDSCAP13747.1
OST UniProtKB Sequence>tr|B0R4T2|B0R4T2_HALS3 Oligosaccharyl transferase OS=Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1) GN=stt3 PE=4 SV=1 MSETTDASGLSAGLPSRALGALKDWYHVPVLGAVLAFMFWVRVQSWDNFLQNGEVYLSGN DAYYHLRQVTYTVHHFPETMPFDPWTNFPHGTLAGQFGTLFDQILAAVALVVGMGSPSEH TIAMTLLLAPPVFGALLVVPTYVMGRRIAGRLGGLFGAVILGLLPGYFLQRTLAGAADHN GAEPLFMTLAVVGLMVALSVAEREKPVFEQFRAWDATGLRDVVGWSVLAGVATAVYMWVW PPGVLLVGIFGAFFLVKLVGDYVTGTSPDHVAVVGAVSMTTTALLMFAPLGESGFGVSGF SFLQPVFALGVAVGCVVLAWLARVFDERSLSPAAYVATVAGTLVVVVGALALVAPGFWSS LQSNLLNYIGLSSTADTRTIGEAQPFLFRTGRYGLGMFGVVFLEYGTAFFSALVGAGAIL LKPHLTSGSVRRIVGVAGATAVLAIVAVSPAVPAAIGGVVGLGGQLTALLIAAVVLAAIA ATGQYDADKLLLVVWGAFVTSAAFTQVRFNYYLVVPVVVLNAYVLRAVLAAVDLDRPVAA IEGVSWYQVGTVVIVALIVLVPVAAPAVAEATNNDSASGPVSPIKLSNNQNQPVPLQSAI TAGQGQGPGGVVAWDDAMDWYQSHTPKQGTYGGADNADALDYNGTYSQTEDFDYPAGSFG VLSWWDYGHWMTVQGHAIPHANPFQQGATSAANFLLADDEQQSESVLADIEEDDAKNRYV AVDWKMVMPPLPYLSSGQSKFSAPVVFYDGPRDLSQGDFYQQAYNADWEENRISNARFFR QSPLLKKQAYYESMMVRLYRYHGSAKQAEPVVADWRRSVNTGQGTAAAFDAATNNTKQFD SMADAKAYVRNDSTA
OST EC Number (BRENDA)2.4.1.119
OST Genome Context
Characterized Accessory Gene(s)
PGL Additional LinksCAZy
Literatures
Reference(s)1) Zeitler, R., Hochmuth, E., Deutzmann, R. and Sumper, M. (1998) Exchange of Ser-4 for Val, Leu or Asn in the sequon Asn-Ala-Ser does not prevent N-glycosylation of the cell surface glycoprotein from Halobacterium halobium. Glycobiology, 8, 1157-1164. [PubMed: 9858637]
Additional CommentsEngineered glycopeptide.
It has been suggested that there may be two different N-glycosyltransferases in H.halobium, one of which does not use the typical consensus sequence Asn-Xaa-Ser/Thr necessary for all other N-glycosyltransferases.
Synthetic oligonucleotides corresponding to nucleotide 100–132 of the csg open reading frame were used to produce the peptides.
While the N-glycosyltransferase acting at N479 is a conventional one requiring the hydroxyamino acid in the N-glycosy
Year of Identification1998
Year of Validation1998