ProGlyProt ID AC101 Organism Information Organism Name Halobacterium salinarum (halobium) R1M1/NRC-1 Domain Archaea Classification Family: Halobacteriaceae Order: Halobacteriales Class: Halobacteria or Halomebacteria Division or phylum: "Euryarchaeota" Taxonomic ID (NCBI) 64091 Genome Sequence (s) GeneBank AE004437.1 EMBL AE004437 Gene Information Gene Name csg (VNG_2679G) NCBI Gene ID 1449008 GenBank Gene Sequence 1449008 Protein Information Protein Name S layer glycopeptide UniProtKB/SwissProt ID Q9HM69 NCBI RefSeq NP_281222.1 EMBL-CDS AAG20702.1 UniProtKB Sequence >sp|Q9HM69|CSG_HALSA Cell surface glycoprotein OS=Halobacterium salinarium GN=csg PE=1 SV=2 MTDTTGKLRAVLLTALMVGSVIGAGVAFTGGAAAANASDLNDYQRFNENTNYTYSTASED GKTEGSVASGATIFQGEEDVTFRKLDNEKEVSPATLSRTGGSDEGVPLQMPIPEDQSTGS YDSNGPDNDEADFGVTVQSPSVTMLEVRNNADNDVTGGVLNTQQDESSIAVDYNYYAAED LELTVEDEDGLDVTDEILAADQSGGAYEDGTGNNGPNTLRFDIDPNNVDAGDYTVSVEGV EDLDFGDATESASVTISSSNKASLNLAEDEVVQGANLKYTIENSPEGNYHAVTIDSSDFR DSSSGADAAKVMRSVGDTVDTGLVVDNDSTTEIVDDYENTSISDVDYAYAIVEIDDGNGV GSIETQYLDDSSADIDLYPASDTEDAPDYVNSNEELTNGSALDGVSTDDDTDFDVTQGDI TLDNPTGAYVVGSEVDINGTANEGTDDVVLYARDNNDFELVTVDGEKSIEVDSDDTFEEE DITLSDGDKGGDDILGLPGTYRLGIIAKSDAVNSSGGVKDNIDTSDFNQGVSSTSSIRVT DTELTASFETYNGQVADDDNQIDVEGTAPGKDNVAAIIIGSRGKVKFQSISVDSDDTFDE EDIDISELRQGSASAHILSSGRDGKFGEDTANSISDLEDEVGNYTSGSPTGDQIRDRILS NTVDDTASDDLIVTQQFRLVDGLTTIEATEGGEAGGSLTVMGTTNRKADDNTITVELLQG DASIEINSTDEWNSDGQWSVDVPLSNVEPGNYTVEADDGDNTDRQNVEIVEELEEPDQTT VDQPENNQTMTTTMTETTTETTTEMTTTQENTTENGSEGTSDGESGGSIPGFGVGVALVA VLGAALLALRQN Sequence length 852 AA Subcellular Location Surface Function Protein Structure PDB ID Glycosylation Status Glycosylation Type N (Asn) linked Experimentally Validated Glycosite(s) in Full Length Protein N2 [Replacement of the Ser residue at the consensus sequence by Val, Leu, and Asn, did not abolish glycosylation of Asn-2. However, replacing Ser-481 in the sequence Asn-Ser-Ser for Val prevented glycosylation of Asn-479.] Experimentally Validated Glycosite(s ) in Mature Protein N2 Glycosite(s) Annotated Protein Sequence AN*(2)AVDLNDYQR (csg mutants with Val, Leu or Asn at 4th position were created) Sequence Around Glycosites (21 AA) Glycosite Sequence Logo seqlogo Glycosite Sequence Logo Technique(s) used for Glycosylation Detection Edman degradation using an automated gas phase sequencer. Technique(s) used for Glycosylated Residue(s) Detection Edman degradation using gas phase sequencing (known residues are mutated). Protein Glycosylation- Implication Glycan Information Glycan Annotation Same sugar pattern like that of the wild-type is present. Galacturonic acid, 3-O-methyl-galacturonic acid, galactose, N-acetylglucosamine, and N-acetylgalactosamine have been observed. Technique(s) used for Glycan Identification Gas chromatographic analysis of pentafluoropropionyl-methyl-glycosides. Protein Glycosylation linked (PGL) gene(s) OST Gene Name aglB/stt3 OST NCBI Gene ID 5953829 OST GenBank Gene Sequence 5953829 OST Protein Name AglB/STT3 subunit OST UniProtKB/ SwissProt ID B0R4T2 OST NCBI RefSeq YP_001689095.1 OST EMBL-CDS CAP13747.1 OST UniProtKB Sequence >tr|B0R4T2|B0R4T2_HALS3 Oligosaccharyl transferase OS=Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1) GN=stt3 PE=4 SV=1 MSETTDASGLSAGLPSRALGALKDWYHVPVLGAVLAFMFWVRVQSWDNFLQNGEVYLSGN DAYYHLRQVTYTVHHFPETMPFDPWTNFPHGTLAGQFGTLFDQILAAVALVVGMGSPSEH TIAMTLLLAPPVFGALLVVPTYVMGRRIAGRLGGLFGAVILGLLPGYFLQRTLAGAADHN GAEPLFMTLAVVGLMVALSVAEREKPVFEQFRAWDATGLRDVVGWSVLAGVATAVYMWVW PPGVLLVGIFGAFFLVKLVGDYVTGTSPDHVAVVGAVSMTTTALLMFAPLGESGFGVSGF SFLQPVFALGVAVGCVVLAWLARVFDERSLSPAAYVATVAGTLVVVVGALALVAPGFWSS LQSNLLNYIGLSSTADTRTIGEAQPFLFRTGRYGLGMFGVVFLEYGTAFFSALVGAGAIL LKPHLTSGSVRRIVGVAGATAVLAIVAVSPAVPAAIGGVVGLGGQLTALLIAAVVLAAIA ATGQYDADKLLLVVWGAFVTSAAFTQVRFNYYLVVPVVVLNAYVLRAVLAAVDLDRPVAA IEGVSWYQVGTVVIVALIVLVPVAAPAVAEATNNDSASGPVSPIKLSNNQNQPVPLQSAI TAGQGQGPGGVVAWDDAMDWYQSHTPKQGTYGGADNADALDYNGTYSQTEDFDYPAGSFG VLSWWDYGHWMTVQGHAIPHANPFQQGATSAANFLLADDEQQSESVLADIEEDDAKNRYV AVDWKMVMPPLPYLSSGQSKFSAPVVFYDGPRDLSQGDFYQQAYNADWEENRISNARFFR QSPLLKKQAYYESMMVRLYRYHGSAKQAEPVVADWRRSVNTGQGTAAAFDAATNNTKQFD SMADAKAYVRNDSTA OST EC Number (BRENDA) 2.4.1.119 OST Genome Context Characterized Accessory Gene(s) PGL Additional Links CAZy Literatures Reference(s) 1) Zeitler, R., Hochmuth, E., Deutzmann, R. and Sumper, M. (1998) Exchange of Ser-4 for Val, Leu or Asn in the sequon Asn-Ala-Ser does not prevent N-glycosylation of the cell surface glycoprotein from Halobacterium halobium. Glycobiology, 8, 1157-1164. [PubMed: 9858637] Additional Comments Engineered glycopeptide. It has been suggested that there may be two different N-glycosyltransferases in H.halobium, one of which does not use the typical consensus sequence Asn-Xaa-Ser/Thr necessary for all other N-glycosyltransferases. Synthetic oligonucleotides corresponding to nucleotide 100–132 of the csg open reading frame were used to produce the peptides. While the N-glycosyltransferase acting at N479 is a conventional one requiring the hydroxyamino acid in the N-glycosy Year of Identification 1998 Year of Validation 1998