| DB ID | MyCo_7160 |
| Title | Endocytic Markers Associated with the Internalization and Processing of Aspergillus fumigatus Conidia by BEAS-2B Cells |
| Year | 2019 |
| PMID | 30728282 |
| Fungal Diseases involved | Aspergillus fumigatus infection |
| Associated Medical Condition | None |
| Genus | Aspergillus |
| Species | fumigatus |
| Organism | Aspergillus fumigatus |
| Ethical Statement | All protocols involving animals were approved by the Institutional Animal Care and Use Committee at Virginia Tech and in compliance with Public Health Service Policy (PHS [approval no. 16-085]). |
| Site of Infection | None |
| Opportunistic invasive | Invasive/Opportunistic |
| Sample type | Biopsy |
| Sample source | Extracted tissue |
| Host Group | Animal |
| Host Common name | Mice |
| Host Scientific name | Mus musculus |
| Biomarker Name | 2xFYVE |
| Biomarker Full Name | 2xFYVE |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | USA |
| Cohort | None |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Aspergillus fumigatus is an opportunistic fungal pathogen capable of invasive pul- monary infection in a diverse array of immunocompromised individuals. Chronic exposure to A. fumigatus may also result in the development and progression of an allergic response, the most severe being acute bronchopulmonary aspergillosis (ABPA). An allergic response occurs predominately in individuals with asthma or cystic fibrosis. A. fumigatus conidia are found abundantly throughout nature and the built environment, making exposure invariable. Most inhaled conidia are thought to be removed by mucociliary clearance in respiring organisms. However, A. fumigatus conidia can easily bypass mucociliary clearance and infiltrate lung alveoli due to their small size (2 to 3 m in diameter). Airway epithelial cells (AECs) make up a large percentage of the pulmonary-air interface and are thought to play an important role in initial conidial recognition, processing, and immune signaling. There is a need to mechanistically understand how A. fumigatus conidia are processed by AECs. |
| Technique | Analytic |
| Analysis Method | Western Blotting |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | Western Blotting, Cell-line-based conidial challenge assays |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
| External Link | None |
