| DB ID | MyCo_6939 |
| Title | Identification of Oil Palm's Consistently Upregulated Genes during Early Infections of Ganoderma boninense via RNA-Seq Technology and Real-Time Quantitative PCR |
| Year | 2021 |
| PMID | 34685835 |
| Fungal Diseases involved | Basal stem rot (BSR) disease |
| Associated Medical Condition | None |
| Genus | Ganoderma |
| Species | boninense |
| Organism | Ganoderma boninense |
| Ethical Statement | None |
| Site of Infection | Root |
| Opportunistic invasive | None |
| Sample type | Plant extracts |
| Sample source | Plant extracts |
| Host Group | Plant |
| Host Common name | Palm |
| Host Scientific name | Arecaceae |
| Biomarker Name | GLUCAN (XLOC_009482 ) |
| Biomarker Full Name | Glucan endo-1,3-beta-glucosidase-like |
| Biomarker Type | Diagnostic |
| Biomolecule | Gene |
| Geographical Location | Malaysia |
| Cohort | The root tissues used for the transcriptome library generation were sampled from 12-month old commercial oil palm seedlings, Tenera (DxP), which were artificially infected with Ganoderma boninense colonized on rubber woodblock (RWB) using a root inoculation technique. The RWBs were inoculated with a 7-day-old pure culture of Ganoderma boninense PER71. G. boninense strain PER71 is an aggressive fungal pathogen that causes BSR. |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | 17.18 |
| Pathway | None |
| Disease Introduction Mechanism | Malaysian oil palm cultivation suffers from a devastating disease known as basal stem rot (BSR), caused by the fungus Ganoderma boninense. BSR is a fatal disease in the oil palm industry in Malaysia. It causes the internal trunk tissue to rot, thus reducing the number of standing palms. Therefore, the threat of BSR disease towards the oil palm industry warrants extensive effort in finding a solution to eradicate this disease permanently. |
| Technique | Bioinformatics analysis |
| Analysis Method | RNA-sequencing (RNA-seq) transcriptomics Approach |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | RNA-sequencing (RNA-seq) transcriptomics Approach, qRT-PCR |
| Up Regulation Down Regulation | Up regulated |
| Sequence Data | XP 010921890.1 |
| External Link | None |
