| DB ID | MyCo_6790 |
| Title | Transcriptional, hormonal, and metabolic changes in susceptible grape berries under powdery mildew infection |
| Year | 2021 |
| PMID | 34106234 |
| Fungal Diseases involved | Powdery Mildew Disease |
| Associated Medical Condition | None |
| Genus | Erysiphe |
| Species | necator |
| Organism | Erysiphe necator |
| Ethical Statement | None |
| Site of Infection | None |
| Opportunistic invasive | None |
| Sample type | Plant extracts |
| Sample source | Extracted RNA |
| Host Group | Plant |
| Host Common name | Grapevine |
| Host Scientific name | Vitis vinifera |
| Biomarker Name | Resveratrol |
| Biomarker Full Name | Resveratrol |
| Biomarker Type | Diagnostic |
| Biomolecule | Metabolite |
| Geographical Location | Germany |
| Cohort | Grape berry clusters (V. vinifera L. cv. Carignan) were collected in 2017 from a commercial vineyard subjected to regular phytosanitary treatments at Torres Vedras region, Portugal (39°04′43.2″N, 9°20′58.9″W). Sampling was performed in two conditions, healthy berries and naturally infected berries, at two developmental stages, late green (EL33) and early véraison (EL35; 25–30% colored berries). For each condition (PM infected and control) and time point, four to five biological replicates (corresponding to four to five clusters from different plants, 20–25 berries from each cluster) were collected on 13 July and 2 August 2017 after visual inspection of symptoms. Grape clusters were harvested around 11.30 h and immediately frozen in liquid nitrogen, transported in dry ice to the laboratory, and stored at −80 °C until further use. Prior to transcriptomic and metabolomic analysis, berries were deseeded and ground. Three to four replicates were used for metabolomics and hormone quantification and some of those samples were pooled to obtain three independent biological replicates for RNA-seq analysis. |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Grapevine (Vitis vinifera L.) is a perennial woody plant highly susceptible to several abiotic and biotic stresses. Powdery mildew (PM) is one of the most dramatic diseases affecting grape production worldwide. It is caused by the ascomycete fungus Erysiphe necator Schw. (syn. Uncinula necator [Schw.] Burr.), an obligate biotrophic fungus that infects berry clusters and predisposes them to bunch rot infections. Eurasia-originated V. vinifera species are more susceptible to PM than the native North American Vitis species. Moreover, resistance to PM was also found in Chinese accessions of non-vinifera species, namely V. romanetti, V. pseudoreticulata and V. piasezkii, as well as in Central Asian accessions of V. vinifera. Since most of the cultivars used for wine and table grape production belong to V. vinifera, PM has spread to all vinicultural regions, and the control strategy is entirely dependent on the widespread application of sulfur-based and synthetic fungicides resulting in environmental poisoning and an impact on health. |
| Technique | Bioinformatics analysis |
| Analysis Method | Transcriptomics |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | Transcriptomics |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
| External Link | None |
