| DB ID | MyCo_6593 |
| Title | (1-3)-beta-D-glucan in association with lactate dehydrogenase as biomarkers of Pneumocystis pneumonia (PcP) in HIV-infected patients |
| Year | 2014 |
| PMID | 24487911 |
| Fungal Diseases involved | Pneumocystis pneumonia |
| Associated Medical Condition | HIV-AIDS-infected patients |
| Genus | Pneumocystis |
| Species | jirovecii |
| Organism | Pneumocystis jirovecii |
| Ethical Statement | The present study had the approval of the Institutional Review Boards/Ethical Committees from the involved institutions (Faculdade de Psicologia e Ciências da Educação, Universidade de Coimbra, CMDT, Coimbra, Portugal). |
| Site of Infection | None |
| Opportunistic invasive | Opportunistic |
| Sample type | Body fluid |
| Sample source | Serum |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | BDG |
| Biomarker Full Name | 1-3-beta-D-Glucan |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | Portugal |
| Cohort | A total of 150 participants (100 patients and 50 healthy blood donors) were included in this study. One hundred pulmonary specimens (65 IS and 35 BAL fluids) and sera were obtained from the 100 HIV-positive adult patients with respiratory symptoms, living in Lisbon, Portugal. These samples were collected between 1998 and 2011 for diagnostic purpose before patients start their anti-P . jirovecii therapeutic regimen with trimethoprim-sulfamethoxazole. |
| Cohort No. | 150 |
| Age Group | None |
| P Value | p<0.001 |
| Sensitivity | 91.30% |
| Specificity | 61.30% |
| Positive Predictive Value | 85.10% |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Pneumocystis jirovecii is an opportunistic pathogen capable of causing fatal interstitial pneumonia in immunocompromised patients. Clinical diagnosis is complex because no clinical symptoms, radiologic features, or gasometric data are specific to Pneumocystis pneumonia (PcP). Since P. jirovecii cannot be cultured reliably, non-culturing methods are used for diagnosis of the disease, including direct cytochemical staining, immunofluorescent staining with monoclonal antibodies (IF), and molecular methods such as PCR. |
| Technique | Assay |
| Analysis Method | Serology Based |
| ELISA kits | Lactate Dehydrogenase Assay Kit (BioVision, CA, USA) |
| Assay Data | FDA- Fungitell®, Cape Cod International, Inc.; Falmounth, MA, USA |
| Validation Techniques used | FDA approved Fungitell assay and Lactate Dehydrogenase Assay |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
| External Link | None |
