| DB ID | MyCo_6523 |
| Title | Applicability of the Platelia EIA® Aspergillus test for the diagnosis of aspergilosis in penguins |
| Year | 2018 |
| PMID | 30133554 |
| Fungal Diseases involved | Aspergillosis |
| Associated Medical Condition | None |
| Genus | Aspergillus |
| Species | spp. |
| Organism | Aspergillus spp. |
| Ethical Statement | None |
| Site of Infection | None |
| Opportunistic invasive | Opportunistic |
| Sample type | Body fluid |
| Sample source | Serum |
| Host Group | Animal |
| Host Common name | Magellanic penguins |
| Host Scientific name | Spheniscus magellanicus |
| Biomarker Name | GM |
| Biomarker Full Name | Galactomannan |
| Biomarker Type | Negative |
| Biomolecule | Protein |
| Geographical Location | Brazil |
| Cohort | They were included in the study, blood serum samples of Magellanic penguins that died of aspergillosis during the rehabilitation period in the Centro de Recuperação de Animais Marinhos of Rio Grande - CRAM-FURG (n=29). The samples are stored in the mycology laboratory- FAMED-FURG, and all cases were confirmed from post-mortem examinations with mycological culture and histopathological examination. As a control group, were included over 23 serum samples from healthy Penguins CRAM-FURG, which they were rehabilitated and released to their natural habitat. All samples were aliquoted in biosafety cabinet to prevent contamination by airborne conidia and found themselves stored at -20 ° C. |
| Cohort No. | 29 |
| Age Group | None |
| P Value | p=0.503 |
| Sensitivity | 86.2 to 34.5% |
| Specificity | 87% and 26.1%. |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Aspergillus species have been known for decades as important pathogens of birds, leading to high mortality rates, including the Spheniscidae family, being about 90-95% of the cases of aspergillosis caused by Aspergillus section Fumigati. The diagnosis ante-mortem of invasive aspergillosis (IA) in birds is limited and traditional techniques, such as blood tests, biochemical tests and imaging studies may reveal only nonspecific changes. Mycological classic tests have low sensitivity and / or specificity, and use of serological tests, although indicated in the literature it is not in routine use, as the gold standard is still restricted to histopathological and mycological post-mortem. |
| Technique | ELISA |
| Analysis Method | sandwich ELISA technique |
| ELISA kits | Platelia Aspergillus EIA ELISA Kit (Bio-Rad USA) |
| Assay Data | None |
| Validation Techniques used | ELISA |
| Up Regulation Down Regulation | No Statistical Difference |
| Sequence Data | None |
| External Link | None |
