| DB ID | MyCo_6481 |
| Title | Diagnostic accuracy of fungal PCR and β-d-glucan for detection of candidaemia: a preliminary evaluation |
| Year | 2017 |
| PMID | 28970296 |
| Fungal Diseases involved | Candidemia |
| Associated Medical Condition | None |
| Genus | Candida |
| Species | albicans |
| Organism | Candida albicans |
| Ethical Statement | None |
| Site of Infection | None |
| Opportunistic invasive | None |
| Sample type | Body fluid |
| Sample source | Serum |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | ITS |
| Biomarker Full Name | Internal Transcribed Spacer |
| Biomarker Type | Diagnostic |
| Biomolecule | Gene |
| Geographical Location | UK |
| Cohort | Specimens from consecutive eligible patients with blood-culture-confirmed candidaemia constituted cases and were identified prospectively, over a 4-month period, in a large university hospital laboratory. A serum specimen was obtained from each case patient on the same day that the first positive blood culture had been drawn (‘paired serum’). These sera had been taken for normal clinical care and were surplus to routine testing requirements. When available, surplus serum specimens that had been obtained the day after the first positive blood culture had been drawn were also retrieved. Inclusion criteria were as follows: candidaemia with an available paired serum specimen of at least 1 mL; age >18 years; not neutropenic. A total of 12 case patients were identified, of which 10 met the inclusion criteria. |
| Cohort No. | 12 |
| Age Group | > 18 |
| P Value | None |
| Sensitivity | 0.444 |
| Specificity | 0.872 |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | The mortality associated with sepsis caused by Candida infection is high and increases with delayed initiation of antifungal therapy. Blood culture is the current standard method for detection of candidaemia and is problematic in that there is a time delay between sampling and obtaining a result. Turnaround time for blood culture is frequently >48 hours and can be up to 5 days. Furthermore, candidaemia is not synonymous with invasive candidosis. Because blood culture fails to diagnose all autopsy-confirmed cases of invasive candidosis, reliance on blood culture positivity leads to underdiagnosis. |
| Technique | PCR |
| Analysis Method | Renishaw RenDx Fungiplex PCR |
| ELISA kits | None |
| Assay Data | FDA- Fungitell® assay (Fungitell, Associates of Cape Cod, USA), RenDx Fungiplex (Renishaw Diagnostics) |
| Validation Techniques used | Renishaw RenDx Fungiplex PCR |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
| External Link | None |
