| DB ID | MyCo_6415 |
| Title | Proteomic characterization of human proinflammatory M1 and anti-inflammatory M2 macrophages and their response to Candida albicans |
| Year | 2014 |
| PMID | 24687989 |
| Fungal Diseases involved | Candida albicans infection |
| Associated Medical Condition | None |
| Genus | Candida |
| Species | albicans |
| Organism | Candida albicans |
| Ethical Statement | None |
| Site of Infection | None |
| Opportunistic invasive | Opportunistic |
| Sample type | Biopsy |
| Sample source | Tissue Supernatant |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | Tpi1 |
| Biomarker Full Name | Triosephosphate isomerase |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | Spain |
| Cohort | The C. albicans strain was a clinical isolate (SC5314), and was maintained on solid yeast extract peptone dextrose medium (1%D-glucose, 1%Difco Yeast Extract, and 2% agar) and incubated at 30̊ C for at least 2 days before use. Human peripheral blood mononuclear cells were isolated from buffy coats of healthy donors over a Lymphoprep (Nycomed Pharma) gradient according to standard procedures. Monocytes were purified from peripheral blood mononuclear cell by magnetic cell sorting using anti-CD14- coated beads (Miltenyi Biotech). To generate M1 (GM-CSF) and M2 (M-CSF) monocyte-derived macrophages, CD14+ cells were cultured with 1000 U/mL rhGM-CSF (Immuno- Tools) or 10 ng/mLM-CSF (ImmunoTools), respectively, and with cytokine addition every two days. After seven days, positive control M1 and M2 macrophages were stimulated with 10 ng/mL LPS and cultured for 24 h before collecting supernatants for cytokine determination. |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Candida albicans is an opportunistic pathogen causing symptomatic infections especially in patients with compromised immune functions. This opportunistic yeast possesses the remarkable ability to survive and proliferate in a changing environment, adapting its growth to physiological extremes of pH, osmolarity, availability of nutrients, and temperature. C. albicans is a commensal fungus present in the skin and mucosal flora in the majority of healthy humans. The transition from unicellular yeast to filamentous forms is a crucial C. albicans virulence factor, and appears to determine tissue invasion and escape from the host immune response. |
| Technique | Bioinformatics analysis |
| Analysis Method | Proteomics Based |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | Quantitative Proteomics, Two-dimensional difference in-gel electrophoresis (2D-DIGE), ELISA, Mass spectrometry, qRT-PCR, Fluorescence microscopy, Western blotting |
| Up Regulation Down Regulation | Differentially Expressed |
| Sequence Data | None |
| External Link | None |
