| DB ID | MyCo_6407 |
| Title | Proteome analysis of Aspergillus flavus isolate-specific responses to oxidative stress in relationship to aflatoxin production capability |
| Year | 2018 |
| PMID | 29467403 |
| Fungal Diseases involved | Aspergillosis |
| Associated Medical Condition | None |
| Genus | Aspergillus |
| Species | flavus |
| Organism | Aspergillus flavus |
| Ethical Statement | None |
| Site of Infection | None |
| Opportunistic invasive | Opportunistic |
| Sample type | Plant extracts |
| Sample source | Plant extracts |
| Host Group | Plant |
| Host Common name | Maize |
| Host Scientific name | Zea mays |
| Biomarker Name | hsp90 |
| Biomarker Full Name | Heat shock protein 90 |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | USA |
| Cohort | The AF13 isolate used in this study was obtained from Dr. Kenneth Damann, Department of Plant Pathology and Crop Physiology, Louisiana State University, Baton Rouge, LA. The NRRL3357 isolate was obtained from the USDA National Culture Repository, Peoria, IL. The K54A isolate was obtained from Dr. Hamed Abbas, USDA-ARS, Mycotoxin Res Unit, Stoneville, MS. All isolates were received on potato dextrose agar (PDA) and were sub cultured on V8 agar (20% V8 juice, 1% CaCO3, 2% agar) prior to use. |
| Cohort No. | None |
| Age Group | None |
| P Value | p≤0.05 |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | ≥1.2 or ≤0.8 |
| Pathway | None |
| Disease Introduction Mechanism | Aspergillus flavus (Link ex Fr, Teleomorph: Petromyces flavus) is a facultative plant pathogen, which is capable of infecting maize and peanut. The infection of these crops by A. flavus poses a serious threat to human and animal health due to its production of carcinogenic mycotoxins, termed as aflatoxins, resulting in contamination of foodstuffs and livestock feed. Aflatoxin contamination also leads to significant economic losses globally due to lost crop value and regulatory restrictions on import and export of contaminated materials. |
| Technique | Bioinformatics analysis |
| Analysis Method | Proteomics Based |
| ELISA kits | iTRAQ Reagents –8-plex kit (AB Sciex Inc., Foster City, CA, USA) |
| Assay Data | None |
| Validation Techniques used | iTRAQ (Isobaric Tags for Relative and Absolute Quantification) Proteomics approach |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
| External Link | None |
