| DB ID | MyCo_5956 |
| Title | Development of an isolate-specific marker for tracking Phaeomoniella chlamydospora infection in grapevines |
| Year | 2005 |
| PMID | 16596959 |
| Fungal Diseases involved | Phaeomoniella chlamydospora infection |
| Associated Medical Condition | None |
| Genus | Phaeomoniella |
| Species | chlamydospora |
| Organism | Phaeomoniella chlamydospora |
| Ethical Statement | None |
| Site of Infection | None |
| Opportunistic invasive | None |
| Sample type | Plant extracts |
| Sample source | Extracted DNA |
| Host Group | Plant |
| Host Common name | Grapevine |
| Host Scientific name | Vitis vinifera |
| Biomarker Name | Isolate A21 of moxY |
| Biomarker Full Name | Isolate A21 of moxY |
| Biomarker Type | Diagnostic |
| Biomolecule | Gene |
| Geographical Location | New Zealand |
| Cohort | Fifty-three isolates of P. chlamydospora and four isolates of Pm. aleophilum were used in this study. For genomic DNA (gDNA) extraction, a spore suspension from each isolate was streaked onto PDA and incubated 4–7 d at 22 C in alternating 12 h light and dark conditions. |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Esca and esca-like syndromes are devastating diseases of mature vines in Europe and North America. The etiology of esca is not understood completely, but it appears to be a complex disease involving the succession of different fungi that culminate in an extensive white rot of the vine trunk (Mugnai 1999). Several fungi are associated with esca-affected grapevines, including Phaeomoniella chlamydospora, Phaeoacremonium aleophilum, to a lesser extent Pm. inflatipes. All species are isolated with a high frequen- cy from discolored woody tissue of esca-affected vines, especially from brown or black longitudinal streaks, where marked gummosis of the xylem elements also occurs. |
| Technique | PCR |
| Analysis Method | PCR-RFLP Approach |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | PCR-RFLP Approach |
| Up Regulation Down Regulation | Positive |
| Sequence Data | GenBank accession number AY921607 |
| External Link | None |
