| DB ID | MyCo_5949 |
| Title | PCR and other non-culture methods for diagnosis of invasive Candida infections in allogeneic bone marrow and solid organ transplant recipients |
| Year | 1999 |
| PMID | 10424090 |
| Fungal Diseases involved | Invasive candidiasis |
| Associated Medical Condition | allogeneic bone marrow and solid organ transplant recipients |
| Genus | Candida |
| Species | spp. |
| Organism | Candida spp. |
| Ethical Statement | None |
| Site of Infection | None |
| Opportunistic invasive | Opportunistic |
| Sample type | Body fluid |
| Sample source | Urine |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | BDG |
| Biomarker Full Name | 1-3-beta-D-Glucan |
| Biomarker Type | Diagnostic |
| Biomolecule | None |
| Geographical Location | Sweden |
| Cohort | From December 1994 to April 1997, 30 recipients of allogeneic bone marrow transplant (BMT) and 10 recipients of solid organ transplants (SOT) (five liver, three kidney, two pancreas and simul-taneously kidney) were prospectively studied. In this prospective study 197 serum and 152 urine samples were collected from 40 bone marrow and solid organ transplant recipients with clinically suspected invasive fungal infection lipid formulation of amphotericin B or fluconazole. |
| Cohort No. | 40 |
| Age Group | 6–63 |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Candida and Aspergillus species are the main causes of invasive fungal infections (IFI) in allogeneic bone marrow and organ transplant recipients, with high mortality rates, even in patients receiving antifungal treatment. IFI continues to be a major challenge for the clinician since diagnosis on clinical grounds is difficult and conventional culture-based methods are slow and insensitive. |
| Technique | PCR |
| Analysis Method | PCR based d/l-arabinitol assays |
| ELISA kits | None |
| Assay Data | BGD Gluspecy kit (Seikagaku Corp., Tokyo, Japan) |
| Validation Techniques used | FDA approved Fungitell assay, PCR based d/l-arabinitol assays |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
| External Link | None |
