| DB ID | MyCo_5947 |
| Title | Variation in virulence of Aspergillus fumigatus strains in a murine model of invasive pulmonary aspergillosis |
| Year | 1996 |
| PMID | 8810945 |
| Fungal Diseases involved | Invasive pulmonary aspergillosis |
| Associated Medical Condition | None |
| Genus | Aspergillus |
| Species | fumigatus |
| Organism | Aspergillus fumigatus |
| Ethical Statement | None |
| Site of Infection | None |
| Opportunistic invasive | Invasive |
| Sample type | Biopsy |
| Sample source | Extracted DNA |
| Host Group | Animal |
| Host Common name | Mice |
| Host Scientific name | Mus musculus |
| Biomarker Name | 0.95-kb Aspergillus DNA fragment |
| Biomarker Full Name | 0.95-kb Aspergillus DNA fragment |
| Biomarker Type | Diagnostic |
| Biomolecule | Gene |
| Geographical Location | France |
| Cohort | Swiss white female mice (OF1, IFAC REDO) weighing 26-28 g were immunosuppressed with hydrocortisone acetate (Lab. Roussel, Paris) 129 mg/kg by subcuta- neous injections on day -4, -2, 0, +3 and +5. On day 0, groups of 5-10 mice were anaesthetised by inhalation with a mixture of absolute ethano1:chlor- oform:ether (1:2:3) and 4 x lo5 A . fumigatus conidia in 30 pl of sterile saline were inoculated intranasally. Mice were kept in an air filter hood and received sterile food and bedding. |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Aspergillus fumigatus is ubiquitous in the environment and its conidia are inhaled frequently by many normal, healthy people, yet only rarely does invasive aspergil- losis develop. Nevertheless, this common saprophyte is life-threatening for immunocompromised patients and the gravity of disease depends particularly on the host’s phagocytic defences. However, the key events involved in pathogenesis of the infection are not yet known. It has been suggested that the production of serine and metallo-proteases plays a role in the pathogenicity of A. fumigatus. |
| Technique | PCR |
| Analysis Method | PCR |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | PCR |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
| External Link | None |
