| DB ID | MyCo_5910 |
| Title | Species-specific detection of Candida tropicalis using evolutionary conserved intein DNA sequences |
| Year | 2018 |
| PMID | 29427451 |
| Fungal Diseases involved | Candida tropicalis infection |
| Associated Medical Condition | None |
| Genus | Candida |
| Species | tropicalis |
| Organism | Candida tropicalis |
| Ethical Statement | None |
| Site of Infection | None |
| Opportunistic invasive | Opportunistic |
| Sample type | Biopsy |
| Sample source | Extracted DNA |
| Host Group | None |
| Host Common name | None |
| Host Scientific name | None |
| Biomarker Name | Intein DNA-vacuolar membrane ATPase (VMA) |
| Biomarker Full Name | Intein DNA-vacuolar membrane ATPase (VMA) |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | India |
| Cohort | Inbase, a curated intein database, was initially analyzed for Candida species with intein sequences (Perler 2002). Three of the species namely, C. parapsilosis, C. tropicalis and C. glabrata were the only Candida species with intein amino acid sequences. Amino acid sequence of C. tropicalis vacuolar membrane ATPase (VMA) were selected and reverse transcribed using the protein query to retrieve the nucleotide sequence. |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Candida tropicalis is the prime non-albicans known to cause local and systemic candidiasis in immunocompromised patients. Human infections caused by C. tropicalis has intensely increased in last few decades. Also, the emergence of multi-drug resistant strains of C. tropicalis have raised the necessity for developing newer antifungal drugs. The antibiotic resistance pattern significantly differs within Candida species and strains, hence, it is exceedingly indispensable to diagnose the causative agent for accurate therapeutic management of candidiasis. |
| Technique | PCR |
| Analysis Method | RT-PCR |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | RT-PCR |
| Up Regulation Down Regulation | Positive |
| Sequence Data | NCBI Accession No: JQ670682.1 |
| External Link | None |
