| DB ID | MyCo_5783 |
| Title | PD-1 Deficiency Promotes Macrophage Activation and T-Helper Cell Type 1/T-Helper Cell Type 17 Response in Pneumocystis Pneumonia |
| Year | 2020 |
| PMID | 32048861 |
| Fungal Diseases involved | Pneumocystis pneumonia |
| Associated Medical Condition | None |
| Genus | Pneumocystis |
| Species | murina |
| Organism | Pneumocystis murina |
| Ethical Statement | The study protocol was approved by the Institutional Review Board/Ethics Committee of Beijing Chao-Yang Hospital (Beijing, China). |
| Site of Infection | Lungs |
| Opportunistic invasive | Opportunistic |
| Sample type | Body fluid |
| Sample source | Bronchoalveolar lavage fluid (BALF) |
| Host Group | Animal |
| Host Common name | Mice |
| Host Scientific name | Mus musculus |
| Biomarker Name | PD-L1 |
| Biomarker Full Name | PD-1/programmed death-ligand 1 |
| Biomarker Type | Diagnostic |
| Biomolecule | Gene |
| Geographical Location | China |
| Cohort | Adult patients diagnosed with non-HIV PCP in Beijing Chao-Yang Hospital were enrolled. Further detailed information is provided in the online supplement. PD-1−/− mice were purchased from the Jackson Laboratory (Bar Harbor, ME, USA). Severe combined immunodeficient (SCID) mice and wild-type (WT) mice were obtained from Vital River Laboratory Animal Co., Ltd. (Beijing, China). The mice were treated with dexamethasone (Dex). Further information is provided in the online supplement. |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Pneumocystis is an opportunistic fungal pathogen able to cause life-threatening pneumonia in immunocompromised hosts. Before the advent of effective antiretroviral and routine prophylaxis therapy, Pneumocystis pneumonia (PCP) was the most common cause of death among human immunodeficiency virus (HIV) patients. Recently, the growth in immunosuppressive therapy has provoked an increase in the incidence of PCP in the non-HIV immunosuppressed population. In addition, non-HIV PCP patients tend to have higher rates of mechanical ventilation and mortality compared with HIV PCP patients. |
| Technique | PCR |
| Analysis Method | qRT-PCR |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | qRT-PCR, Flow cytometry, Nitrite and H2O2 Assays, Pneumocystis-specific Serum IgG and IgE Analysis, TaqMan RT-PCR, Phagocytosis Assay |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
| External Link | None |
