| DB ID | MyCo_5779 |
| Title | Pneumocystis infection alters the activation state of pulmonary macrophages |
| Year | 2016 |
| PMID | 27720434 |
| Fungal Diseases involved | Pneumocystis infection |
| Associated Medical Condition | None |
| Genus | Pneumocystis |
| Species | jirovecii |
| Organism | Pneumocystis jirovecii |
| Ethical Statement | All experimental animal studies were approved by the University of Kentucky Institutional Animal Care and Use Committee and were conducted in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals. |
| Site of Infection | None |
| Opportunistic invasive | Opportunistic |
| Sample type | Body fluid |
| Sample source | Lungs lavage |
| Host Group | Animal |
| Host Common name | Mice |
| Host Scientific name | Mus musculus |
| Biomarker Name | IL-10 |
| Biomarker Full Name | Interleukin-10 |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | USA |
| Cohort | Five- to 8-wk-old C57BL/6, BALB/c, and BALB/c-Il4ratCAMSz/J (IL-4RĪ±ā/ā) mice were obtained from Jackson Laboratories, bred, and maintained at the AALAC accredited University of Kentucky Animal Facility and were used in the study. |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Pneumocystis jiroveci is an opportunistic fungal pathogen that often causes pneumonia in immunocompromised patients. Recently it was recognized that colonization with Pneumocystis is associated with a decline in pulmonary function in smokers and patients with severe forms of chronic obstructive pulmonary disease (COPD) as well as cystic fibrosis (CF). In addition to colonization and infection with multiple microorganisms, these patients have the hallmarks of chronic lung diseases (airway inflammation, airway remodeling, and parenchymal damage). This creates a complex microenvironment that complicates our understanding of the role of microbes during the development of pulmonary damage and repair. It is unknown whether the presence of Pneumocystis alters the homeostatic set point of immune activation, nor how this influences subsequent pulmonary insults. |
| Technique | PCR |
| Analysis Method | qRT-PCR |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | Flow Cytometry Analysis, qRT-PCR |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
| External Link | None |
