| DB ID | MyCo_5770 |
| Title | An NMRA-Like Protein Regulates Gene Expression in Phytophthora capsici to Drive the Infection Cycle on Tomato |
| Year | 2018 |
| PMID | 29419371 |
| Fungal Diseases involved | Phytophthora Fungal infection |
| Associated Medical Condition | None |
| Genus | Phytophthora |
| Species | capsici |
| Organism | Phytophthora capsici |
| Ethical Statement | None |
| Site of Infection | Leaves |
| Opportunistic invasive | None |
| Sample type | Plant extracts |
| Sample source | Plant extracts |
| Host Group | Plant |
| Host Common name | Tomato |
| Host Scientific name | Solanum lycopersicum |
| Biomarker Name | PcNMRAL1 |
| Biomarker Full Name | PcNMRAL1 |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | UK |
| Cohort | P. capsici susceptible tomato plants (S. lycoperisum cv. Moneymaker) were grown in a greenhouse at 22-25 oC with 16 h light. P. capsici strains (LT6535 and LT1534) were grown on V8 agar medium in the dark at 25 oC for 3 days and subsequently at 22 oC in the light to induce sporangia formation. To harvest sporangia and induce zoospore release, plates were flooded with ice cold water, agitated with a plate spreader to dislodge sporangia, and the sporangia suspension incubated at 22 oC in the light until motile zoospores were released. Zoospores were diluted to a concentration of 1 x 105 spores ml-1 and used to inoculate 4-5 week old detached tomato leaves by placing 20 µl droplets onto the abaxial surface. Leaves were kept in a box lined with moist tissue to create a humid environment, and incubated at 22 oC with a 16 h photoperiod for the duration of the experiment. For lesion measurements, the diameter of the lesion was measured with a ruler. For RNA or protein extraction from infected leaf tissue, leaf discs containing the inoculation site were collected using a core borer (5 mm diameter) and flash frozen in liquid nitrogen before extraction. |
| Cohort No. | None |
| Age Group | None |
| P Value | p<0.005 |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Plant pathogenic oomycetes cause devastating diseases on a wide range of plants important in food production, forestry and natural ecosystems. For example, Phytophthora infestans, the causal agent of late blight on potato and tomato, continues to cause multi-billion dollar losses each year (Birch and Whisson 2001; Nowicki et al. 2012). Other economically devastating pathogens in the genus include P. sojae and P. capsici, the major disease- causing agents of soybean and pepper, respectively. The vast economic damage that Phytophthora pathogens cause, have been and continue to drive efforts to understand the basic processes involved in pathogenicity. |
| Technique | PCR |
| Analysis Method | qRT-PCR |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | qRT-PCR, Western Blot, Microarray |
| Up Regulation Down Regulation | Overexpressed |
| Sequence Data | None |
| External Link | None |
