| DB ID | MyCo_5767 |
| Title | Proteomic analysis reveals that iron availability alters the metabolic status of the pathogenic fungus Paracoccidioides brasiliensis |
| Year | 2011 |
| PMID | 21829521 |
| Fungal Diseases involved | Paracoccidioidomycosis |
| Associated Medical Condition | None |
| Genus | Paracoccidioides |
| Species | brasiliensis |
| Organism | Paracoccidioides brasiliensis |
| Ethical Statement | The anima protocol was approved by the Universidade Federal de Goia ´s committee of the ethical treatment of animals (Number: 131/ 2008). |
| Site of Infection | None |
| Opportunistic invasive | None |
| Sample type | Biopsy |
| Sample source | Homogenized tissue extracts |
| Host Group | Animal |
| Host Common name | Mice |
| Host Scientific name | Mus musculus |
| Biomarker Name | Sit1 |
| Biomarker Full Name | Sit1 |
| Biomarker Type | Diagnostic |
| Biomolecule | Gene |
| Geographical Location | Brazil |
| Cohort | To investigate the effect of iron availability on P. brasiliensis colonization we infected BALB/c mice that were iron supple- mented. All animal experiments were performed in accordance with the international rules for animal experimentation. Thirty day old female BALB/c mice (n = 4) were injected intraperitoneally with a solution containing Ferrodex diluted in 0.9% NaCl (w/v; 50 mg/kg; Tortuga Companhia Zoote ´cnica Agra ´ria, Brazil). |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | None |
| Technique | PCR |
| Analysis Method | qRT-PCR |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | qRT-PCR, 2D-gel electrophoresis, Enzyme activity assays, Mass Spectrometry analysis, Western blot analysis |
| Up Regulation Down Regulation | Up regulated |
| Sequence Data | None |
| External Link | None |
