| DB ID | MyCo_5762 |
| Title | Targeting essential genes of Nosema for the diagnosis of pebrine disease in silkworms |
| Year | 2020 |
| PMID | 33128512 |
| Fungal Diseases involved | Pebrine disease |
| Associated Medical Condition | None |
| Genus | Nosema |
| Species | bombycis |
| Organism | Nosema bombycis |
| Ethical Statement | None |
| Site of Infection | None |
| Opportunistic invasive | None |
| Sample type | Biopsy |
| Sample source | Extracted DNA |
| Host Group | Animal |
| Host Common name | Silkworm |
| Host Scientific name | Bombyx mori |
| Biomarker Name | β-tubulin |
| Biomarker Full Name | β-tubulin |
| Biomarker Type | Diagnostic |
| Biomolecule | Gene |
| Geographical Location | India |
| Cohort | The Nosema spores (from Nosema bombycis, Nosema mylitta, Nosema assamensis, Nosema ricini) were collected from infected Bombyx mori, Antheraea mylitta, Antheraea assama and Samia ricini silkworms respectively from different geographical regions of India such as Karnataka, Assam, Manipur and Tamil Nadu. Infected silk moths around 20–30 in number were crushed in 0.6% potassium carbonate (K2CO3) solution using a sterile mortar and pestle. |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Like any other organism, silkworms are also prone to various diseases and insect infestation which impose severe threat to the silkworm rearing. Nosema, a Microsporidia is one among the opportunistic pathogen which causes a devastating disease called as “pebrine” (also called as microsporidiosis or pepper disease) in silkworms leading to huge crop losses (~36%), affecting sericulture economics. Microsporidians have evolved over many years and possess unique ability to steal host genes as well as nutrients, causing immunodepletion and severe crisis leading to death of host. They were initially considered to be primitive eukaryotes and currently classified as Fungi owning to their peculiar genome and biology. They are noxious to almost all the vertebrates and invertebrates including humans. Nosema bombycis was the first microsporidian to be identified as entomopathogen infecting silkworms. |
| Technique | PCR |
| Analysis Method | qRT-PCR |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | qRT-PCR |
| Up Regulation Down Regulation | Positive |
| Sequence Data | AccNo: DQ663475 |
| External Link | None |
