| DB ID | MyCo_5623 |
| Title | Aspergillus fumigatus promotes T helper type 2 responses through thymic stromal lymphopoietin production by human corneal epithelial cells |
| Year | 2016 |
| PMID | 26759304 |
| Fungal Diseases involved | Fungal keratitis |
| Associated Medical Condition | None |
| Genus | Aspergillus |
| Species | fumigatus |
| Organism | Aspergillus fumigatus |
| Ethical Statement | Ethical approval was obtained from the Medical Ethical Committee of Qilu Hospital, Shandong University. All volunteers provided informed consent. |
| Site of Infection | Eye |
| Opportunistic invasive | Opportunistic |
| Sample type | Macromolecule |
| Sample source | Extracted RNA |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | IL-13 |
| Biomarker Full Name | Interleukin-13 |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | China |
| Cohort | The A. fumigatus strain CCTCC 93024, purchased from the China Centre for Type Culture Collection, was cultured on Sabouraud dextrose agar (BD Diagnostic Systems, Sparks, MD, USA) for 24h at 37 °C on a shaking table with a rotation speed of 200 rpm. Human corneal epithelial cells, an SV40-transformed cell line, were kindly provided by Dr Fu-Shin Yu of Wayne State University in the USA and maintained in Dulbecco’s modified Eagle’s medium/F12 (Invitrogen-Gibco, Carlsbad, CA, USA) supplemented with 50% defined keratinocyte serum-free medium (Invitrogen-Gibco, Carlsbad, CA, USA) at 37 °C in a humidified 5% CO2 incubator. The siRNA oligonucleotides for TSLP were designed and synthesized by GenePharma Corporation (Shanghai, China). Peripheral blood mononuclear cells (PBMCs) from healthy volunteers (10 men and 8 women with mean age 29 ± 6.04 years) taken from ethylene diamine tetraacetic acid blood were purified by Ficoll- Hypaque density gradient centrifugation. |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Fungal keratitis (FK) is a potentially visionthreatening corneal infection, which accounts for about 65% of all corneal ulcers worldwide. FK usually follows vegetal trauma and is becoming the first indication for corneal transplantation in China. One of the main etiologic agents of FK is Aspergillus fumigatus (A. fumigatus). Due to glucocorticoid abuse, delayed diagnosis, limited antifungal medications and low drug sensitivities, FK is still one of the major intractable diseases and recently has been increasing in both the USA and China. If not effectively treated, it can lead to blindness or even loss of the eyeball. In recent years, comprehensive researches have been carried out on corneal innate immunity to FK. Corneal epithelial cells are the first line of defence against fungal infection, recognizing pathogen-associated molecular patterns through pattern recognition receptors, which include toll-like receptors (TLRs), nucleotide-binding oligomerization domain-like receptors and C-type lectin receptors. Crosstalk among TLRs, nucleotide-binding oligomerization domains and CLRs can enhance nuclear factor κB activation, induce inflammatory factors and antimicrobial peptides release, such as IL-1β, IL-6, IL-8, tumour necrosis factor α, human betadefensin 2 and LL37. |
| Technique | PCR |
| Analysis Method | qRT-PCR |
| ELISA kits | ELISA Kit (KYM Co., Ltd, Beijing, China). |
| Assay Data | None |
| Validation Techniques used | ELISA, qRT-PCR, Flow Cytometry |
| Up Regulation Down Regulation | Increase |
| Sequence Data | None |
| External Link | None |
