| DB ID | MyCo_5620 |
| Title | Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis |
| Year | 2012 |
| PMID | 22731696 |
| Fungal Diseases involved | Chronic necrotizing pulmonary aspergillosis |
| Associated Medical Condition | None |
| Genus | Aspergillus |
| Species | fumigatus |
| Organism | Aspergillus fumigatus |
| Ethical Statement | Informed consent was obtained from patients and controls, and the study was approved by Regional Committee for Medical and Research Ethics, South-East, Norway. |
| Site of Infection | Lungs |
| Opportunistic invasive | Opportunistic |
| Sample type | Biopsy |
| Sample source | Extracted RNA |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | IL-10 |
| Biomarker Full Name | Interleukin-10 |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | Norway |
| Cohort | Between January 2002 and January 2008, 10 patients referred to the Department of Respiratory Medicine, Rik-shospitalet, Oslo University Hospital, where a diagnosis of CNPA were established, were included in the study. The study population consisted of two women and eight men, ranging from 52 to 78 years of age (median 63 years). |
| Cohort No. | 29 |
| Age Group | 63 |
| P Value | p<0.001 |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Chronic necrotizing pulmonary Aspergillosis (CNPA) is a locally destructive process of the lung due to semi invasive infection by Aspergillus species often accompanied by development of a fungus ball (aspergilloma). CNPA which is a relatively uncommon form of Aspergillus infection, typically occurs as a complication of preexisting chronic lung diseases such as emphysema, sarcoidosis, tuberculosis sequela and other fibrotic lung diseases as well as in mildly immunocompromised patients e.g. diabetes mellitus, chronic liver disease and alcoholism. The most common symptoms are chronic, productive cough with or without hemoptysis and pleuritic chest pain, but constitutional symptoms (i.e. weight loss, fever and lack of energy) are also seen. Although a few reports suggest a dysregulated cytokine response and involvement of complement activation in CNPA, little is known about the immunopathogenic mechanisms of this disorder. Moreover, whereas thrombosis and vascular invasion are histopathological hallmarks of invasive aspergillosis in severely immunocompromised patients, the role of platelet and endothelial cell activation as well as the levels of prothrombotic mediators in CNPA are largely unknown. |
| Technique | PCR |
| Analysis Method | qRT-PCR |
| ELISA kits | ELISA Bio-Plex Pro Human 8-plex express assay (Bio-Rad Laboratories, Hercules, CA), ELISA enzyme immunoassays (EIAs) [R&D Systems (Minneapolis, MN)], ELISA EIAs (American Diagnostica, Stamford, CT), ELISA EIAs Dako (Glostrup, Denmark) and Bender MedSystems (Vienna, Austria). |
| Assay Data | None |
| Validation Techniques used | ELISA, qRT-PCR |
| Up Regulation Down Regulation | Increase |
| Sequence Data | None |
| External Link | None |
