| DB ID | MyCo_5598 |
| Title | Could aflatoxin B1 production by Aspergillus flavus affect the severity of keratitis: an experience in two tertiary health care centers, Egypt |
| Year | 2019 |
| PMID | 31332609 |
| Fungal Diseases involved | Fungal keratitis |
| Associated Medical Condition | None |
| Genus | Aspergillus |
| Species | flavus |
| Organism | Aspergillus flavus |
| Ethical Statement | None |
| Site of Infection | Eye |
| Opportunistic invasive | None |
| Sample type | Biopsy |
| Sample source | Corneal samples |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | AFB1 |
| Biomarker Full Name | AFB1 |
| Biomarker Type | Diagnostic |
| Biomolecule | Gene |
| Geographical Location | Egypt |
| Cohort | A total of 843 corneal scrapping samples from patients seeking medical advice in outpatient clinics of two ophthalmic tertiary centers in Mansoura, Egypt, were obtained. Samples were collected under complete aseptic precautions using sterile disposable blades. Those patients showing evidence of infective keratitis were enrolled in this study. |
| Cohort No. | None |
| Age Group | None |
| P Value | p<0.0001 |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | One of the most important ophthalmic problems that cause visual disability is mycotic keratitis. Increasing fungal infections in the last 30 years was due to immunodeficiency disorders, misuse and overuse of antibiotics to treat bacterial infections, and the wide dependence on indwelling devices, besides the worldwide spreading of the use of contact lenses. Aspergillus flavus is considered an important etiology of keratitis second to Aspergillus fumigatus. Aspergillus flavus produces potent mycotoxins; aflatoxins, which can cause acute hepatic damage, liver cirrhosis, and tumors and has teratogenic effects. |
| Technique | PCR |
| Analysis Method | qRT-PCR |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | qRT-PCR |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
| External Link | None |
