| DB ID | MyCo_5593 |
| Title | Detection of a molecular biomarker for zygomycetes by quantitative PCR assays of plasma, bronchoalveolar lavage, and lung tissue in a rabbit model of experimental pulmonary zygomycosis |
| Year | 2008 |
| PMID | 18845827 |
| Fungal Diseases involved | Pulmonary Zygomycosis |
| Associated Medical Condition | None |
| Genus | Rhizopus |
| Species | oryzae |
| Organism | Rhizopus oryzae |
| Ethical Statement | All rabbits were individually housed and monitored under humane care and use of standards in facilities accredited by the Association for Assessment and Accreditation of Laboratory Animal Care International and according to the guidelines of the National Research Council for the care and use of laboratory animals and under approval by the Animal Care and Use Committee of the National Cancer Institute. |
| Site of Infection | None |
| Opportunistic invasive | Opportunistic |
| Sample type | Body fluid |
| Sample source | Bronchoalveolar lavage fluid (BALF) |
| Host Group | Animal |
| Host Common name | Rabbit |
| Host Scientific name | Oryctolagus cuniculus |
| Biomarker Name | 28S rRNA gene |
| Biomarker Full Name | 28S rRNA gene |
| Biomarker Type | Diagnostic |
| Biomolecule | Gene |
| Geographical Location | USA |
| Cohort | Both qPCR assays were designed around the 28S rRNA gene in order to optimize the specificity of the assay. This was facilitated by the relative com- pleteness of the sequence data available on the 28S region of the rRNA gene complex. All rabbits were individually housed and monitored under humane care and use of standards in facilities accredited by the Association for Assessment and Accreditation of Laboratory Animal Care International and according to the guidelines of the National Research Council for the care and use of laboratory animals and under approval by the Animal Care and Use Committee of the National Cancer Institute. |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | The number of cases of zygomycosis have increased over the last six decades, making the diagnosis of these infections im- perative. Infections with Zygomycetes may advance rapidly, leading to fatality, particularly in patients with under- lying illnesses such as diabetes, bone marrow or solid-organ transplantation, and renal failure. The modes of transmis- sion are varied for the immunocompromised patient, but the inhalation of sporangiospores with subsequent pulmonary in- fection is the most common. A recent review reported an overall mortality of 76% for patients with pulmonary infec- tions due to Zygomycetes. Furthermore, in the immuno- compromised host, hematogenous dissemination is frequent and contributes to the high mortality rate, underscoring the urgency of making a rapid and accurate diagnosis of pulmo-nary zygomycosis. |
| Technique | PCR |
| Analysis Method | qPCR |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | qPCR |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
| External Link | None |
