| DB ID | MyCo_5589 |
| Title | Increased virulence of Cunninghamella bertholletiae in experimental pulmonary mucormycosis: correlation with circulating molecular biomarkers, sporangiospore germination and hyphal metabolism |
| Year | 2013 |
| PMID | 22686246 |
| Fungal Diseases involved | Pulmonary mucormycosis |
| Associated Medical Condition | None |
| Genus | Rhizopus |
| Species | microsporus |
| Organism | Rhizopus microsporus |
| Ethical Statement | All rabbits were monitored under humane care and use standards in facilities accredited by the Asso- ciation for Assessment and Accreditation of Laboratory Animal Care International, and in accord with the guide- lines of the National Research Council for the care and use of laboratory animals, and under approval by the Animal Care and Use committee of the National Cancer Institute. |
| Site of Infection | None |
| Opportunistic invasive | Opportunistic |
| Sample type | Body fluid |
| Sample source | Serum |
| Host Group | Animal |
| Host Common name | Rabbit |
| Host Scientific name | Oryctolagus cuniculus |
| Biomarker Name | 28S rRNA gene |
| Biomarker Full Name | 28S rRNA gene |
| Biomarker Type | Diagnostic |
| Biomolecule | Gene |
| Geographical Location | Greece |
| Cohort | Two isolates of each of the following species Cunningha- mella bertholletiae (CB; NIH-182 and NIH-190), Rhizopus oryzae (RO; NIH-122 and NIH-176), and Mucor circinel- loides (MC; NIH-184 and NIH-234) and one isolate of R. microsporus (RM; NIH-230) were used in all experiments. There were no signifi cant inter-isolate variations among individual species and each was recovered from a patient with deep invasive mucormycosis. Healthy female New Zealand white rabbits (Covance Research Products, Inc., Denver, PA, USA) weighing 2.6 – 3.5 kg at the time of endotracheal inoculation were used in all experiments. All rabbits were monitored under humane care and use standards in facilities accredited by the Asso- ciation for Assessment and Accreditation of Laboratory Animal Care International, and in accord with the guide- lines of the National Research Council for the care and use of laboratory animals, and under approval by the Animal Care and Use committee of the National Cancer Institute. |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Invasive pulmonary mucormycosis (IPM) is an emerging life-threatening infection in hematopoietic stem cell trans-plant recipients, patients with hematological malignancies, and solid organ transplant recipients. Within the order of Mucorales, Rhizopus oryzae and R. microsporus are the most common species causing IPM. Mucor spp. and Cun- ninghamella spp. are less common but medically important causes of such infections. |
| Technique | PCR |
| Analysis Method | qPCR |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | qPCR |
| Up Regulation Down Regulation | Increase |
| Sequence Data | None |
| External Link | None |
