| DB ID | MyCo_5580 |
| Title | Detection of circulating fungal DNA by polymerase chain reaction in a fatal case of Cunninghamella bertholletiae infection |
| Year | 2020 |
| PMID | 32461900 |
| Fungal Diseases involved | Mucormycosis |
| Associated Medical Condition | Refractory osteosarcoma |
| Genus | Cunninghamella |
| Species | bertholletiae |
| Organism | Cunninghamella bertholletiae |
| Ethical Statement | All procedures performed in this study involving human participants were approved by the ethical review committee of the Kyoto Prefectural University of Medicine. |
| Site of Infection | None |
| Opportunistic invasive | Opportunistic |
| Sample type | Biopsy |
| Sample source | Extracted DNA |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | 146-bp portion of the D1/D2 region |
| Biomarker Full Name | 146-bp portion of the D1/D2 region |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | Japan |
| Cohort | A 23-year-old male with refractory osteosarcoma was admitted with multiple lung metastases. He was on oral voriconazole prophylaxis after pulmonary aspergillosis. He suffered from fever during temporary neutropenia following chemotherapy and showed several neurological and respiratory symptoms. Despite liposomal-amphotericin B administration, the symptoms rapidly progressed, and he died ve days after the onset of neurological symptoms. |
| Cohort No. | 1 |
| Age Group | 23 |
| P Value | p<0.05 |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | The incidence of mucormycosis has been increasing in the last two decades among immunocompromised patients. The average annual incidence rate of mucormycosis increases with age from 0.3/million in children aged 0–9 years to 3.9/million in patients aged > 89 years. Patients with diabetes and malignancies, and those receiving immunosuppressive agents, deferoxamine therapy, or broad-spectrum antimicrobial drugs are at the highest risk of zygomycosis (mucormycosis). The overall mortality rate due to mucormycosis has been reported at almost 100 % in disseminated subtypes, 85 % in gastrointestinal subtypes, 76 % or more in pulmonary subtypes, 62 % in rhinocerebral subtypes, and 10 % in cutaneous subtypes. Cunninghamella bertholletiae rarely causes mucormycosis, but it is responsible for the highest mortality among all mucormycetes. Despite its increasing frequency, mucormycosis remains challenging to diagnose because the fungi belonging to the order Mucorales are dif cult to culture and have no specific biomarker. |
| Technique | PCR |
| Analysis Method | qPCR |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | qPCR |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
| External Link | None |
