| DB ID | MyCo_5567 |
| Title | A Comprehensive in Silico Analysis of Regulatory SNPs of Human CLEC7A Gene and Its Validation as Genotypic and Phenotypic Disease Marker in Recurrent Vulvovaginal Infections |
| Year | 2018 |
| PMID | 29616193 |
| Fungal Diseases involved | Vulvovaginal candidiasis |
| Associated Medical Condition | Recurrent Vulvovaginal infections (RVVI) |
| Genus | None |
| Species | None |
| Organism | None |
| Ethical Statement | The present study was commenced after getting approval from the Institutional Ethics Committee (Approval no. 06/HG dated 02/01/2015) of Guru Nanak Dev University, Amritsar (Punjab), India, in accordance with Indian Council of Medical Research guidelines (ICMR, 2006)modified fromWorld Medical Association (2004). Voluntary consent in written was attained from all the subjects. To ensure that subjects involved in the study had necessary information to make an informed choice the information provided to them include comprehensive depiction of the present study purpose, confirmation of secrecy of their provided information, vaginal, and blood samples collection procedures and possible risks and benefits of the study. The necessary information was recorded in a pre-designed Proforma. In addition, the bio-medical waste was segregated and managed on the basis of color coding as mentioned in Bio-Medical Waste (Management & Handling) Rules, 1998 (amended in 2000). |
| Site of Infection | Vagina |
| Opportunistic invasive | None |
| Sample type | Body fluid |
| Sample source | Peripheral blood samples |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | MBL2 |
| Biomarker Full Name | Mannose binding Lectin 2 |
| Biomarker Type | Predictive |
| Biomolecule | Gene |
| Geographical Location | India |
| Cohort | The study included 258 RVVI cases (Age, mean |
| Cohort No. | 258 Patients |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Vulvovaginal infections (VVI) are the commonly reported microbiological syndrome affecting millions of women globally in all strata of society. An abnormal vaginal discharge is a key trait of VVI and its estimated prevalence in India is 30%. Bacterial Vaginosis (BV), Vulvovaginal Candidiasis (VVC), and Trichomoniasis (TV) are the three main causes of VVI. Mutual existence of these causes is termed as Mixed Infections (MI) that contribute >20% of women with VVI. Besides this, cases of recurrent VVI (RVVI) have also emerged that is commonly stated as repeated experiences of vaginal infections in a definite period, this includes recurrent VVC (RVVC) and recurrent BV (RBV). RVVC refers to ≥4 repeated episodes of VVC in 12-months while RBV refers to the repeated episodes of BV within 3 months with recurrence rates as high as 30–50%. Literature regarding RVVI pathogenesis suggests that it is caused by a fall in hydrogen peroxide producing lactobacilli and overgrowth of microbes that are either normally present in human vaginal microbiome in lower quantity or sexually transmitted. In addition, use of contraceptives, excessive antibiotics, smoking, sexual activity, immunosuppression, and black race are the other known predisposing factors that leads to RVVI. Untreated RVVI can lead to complications like infertility, pre-term birth, miscarriage, vulvovaginal inflammation, and other infectious diseases. Though diverse microbial strains have been defined in literature as a causative agent for RVVI, till date no major determinants have been recognized that could explain susceptibility to this disease condition. So, it was hypothesized that RVVI susceptibility may be determined by individual’s own genetic factors. |
| Technique | PCR |
| Analysis Method | polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP) |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | ELISA, PCR-RFLP |
| Up Regulation Down Regulation | Decrease |
| Sequence Data | None |
| External Link | None |
