| DB ID | MyCo_5484 |
| Title | Usefulness of molecular markers in the diagnosis of occupational and recreational histoplasmosis outbreaks |
| Year | 2016 |
| PMID | 27726064 |
| Fungal Diseases involved | Histoplasmosis |
| Associated Medical Condition | None |
| Genus | Histoplasma |
| Species | capsulatum |
| Organism | Histoplasma capsulatum |
| Ethical Statement | Animal manipulations were in accordance with the suggestions of the Ethics Committee of the School of Medicine, UNAM, and following the recommendations of the Animal Care and Use Committee of the UNAM and the Mexican Official Guide (NOM 062-ZOO-1999). |
| Site of Infection | None |
| Opportunistic invasive | Opportunistic |
| Sample type | Macromolecule |
| Sample source | Extracted DNA |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | Hcp100 |
| Biomarker Full Name | Hcp100 |
| Biomarker Type | Diagnostic |
| Biomolecule | Gene |
| Geographical Location | Mexico |
| Cohort | In this study, clinical (serum) and environmental samples (moist soil containing organic matter) were analysed. These samples were collected during three histoplasmosis outbreaks between 2012 and 2013 in the Mexican states of Morelos, San Luis Potosí and Chiapas. The probable clinical diagnosis of the seven patients involved in the outbreaks was PPH. |
| Cohort No. | 7 |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Histoplasmosis is an endemic mycosis in the Americas, particularly in the USA. However, it frequently occurs as the epidemic form of the disease, such as in local outbreaks in North, Central and South America, where histoplasmosis has been reported between parallels 54° North and 38° South. In Mexico, the disease has been reported across the entire republic, with predominance in the central states, followed by the South Pacific and Gulf of Mexico. This disease is caused by the inhalation of mycelial propagules (mainly microconidia and hyphae fragments) of the dimorphic fungus Histoplasma capsulatum, which is found in moist soils, mainly in those containing bird and/or bat guano. Histoplasmosis can remain asymptomatic or present different clinical forms, from mild or moderate primary lung infection to widespread severe infection in immunocompromised or in immunocompetent patients who inhale a massive dose of the infectious propagules. |
| Technique | PCR |
| Analysis Method | Nested PCR |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | ELISA, PCR |
| Up Regulation Down Regulation | None |
| Sequence Data | GenBank accession no. AJ005963 |
| External Link | GenBank accession no. AJ005963 |
