| DB ID | MyCo_5482 |
| Title | Internal transcribed spacer 2 amplicon as a molecular marker for identification of Peronospora parasitica (crucifer downy mildew) |
| Year | 2004 |
| PMID | 14962138 |
| Fungal Diseases involved | Peronospora parasitica infection |
| Associated Medical Condition | None |
| Genus | Peronospora |
| Species | parasitica |
| Organism | Peronospora parasitica |
| Ethical Statement | None |
| Site of Infection | None |
| Opportunistic invasive | None |
| Sample type | Plant extracts |
| Sample source | Extracted DNA |
| Host Group | Plant |
| Host Common name | Cabbage |
| Host Scientific name | Brassica oleracea |
| Biomarker Name | ITS |
| Biomarker Full Name | Internal Transcribed Spacer |
| Biomarker Type | Diagnostic |
| Biomolecule | Gene |
| Geographical Location | Portugal |
| Cohort | Forty-four P. parasitica isolates, collected from B. oleracea plants, were grown on seedlings of B. oleracea hosts |
| Cohort No. | 44 |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Peronospora parasitica is an exclusively biotrophic oomycete responsible for crucifer downy mildew, one of the most important diseases of brassica crops worldwide. Brassicas are economically very important all over the world, Brassica oleracea being the most cultivated species in the Western Hemisphere, whereas B. campestris predomin- ates Asia. Between 1989 and 1991, world brassica crops represented 16 69 000 ha, increasing to 19 83 000 ha in 1999 (FAO 1999). Although field plants are also severely affected, crucifer downy mildew damages are particularly important in the nursery, when the infection may kill the seedlings, retard their development or cause lack of uniformity and quality. |
| Technique | PCR |
| Analysis Method | Mutiplex PCR |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | Mutiplex PCR |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
| External Link | None |
