| DB ID | MyCo_5473 |
| Title | Assessment of Aspergillus-specific PCR as a screening method for invasive aspergillosis in paediatric cancer patients and allogeneic haematopoietic stem cell recipients with suspected infections |
| Year | 2014 |
| PMID | 24655146 |
| Fungal Diseases involved | Invasive aspergillosis |
| Associated Medical Condition | Paediatric cancer patients and allogeneic haematopoietic stem cell recipients with suspected infections |
| Genus | Aspergillus |
| Species | spp. |
| Organism | Aspergillus spp. |
| Ethical Statement | None |
| Site of Infection | None |
| Opportunistic invasive | Invasive |
| Sample type | Body fluid |
| Sample source | Blood |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | 18S rRNA gene |
| Biomarker Full Name | 18S rRNA gene |
| Biomarker Type | Diagnostic |
| Biomolecule | Gene |
| Geographical Location | Germany |
| Cohort | The study was performed on a cohort of 95 unselected paediatric pts (male: 46, female: 49) of a median age of 9.5 years (range 0.5–20.5 years) and 253 episodes of suspected infections were analysed. Patient characteristics. |
| Cohort No. | 95 |
| Age Group | 0.5-20.5 |
| P Value | p<0.001 |
| Sensitivity | 0.34 |
| Specificity | 0.78 |
| Positive Predictive Value | 0.31 |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Invasive aspergillosis (IA) is a severe, life-threatening complication in immunocompromised patients with a major impact on morbidity and mortality. As the majority of data is derived from clinical studies involving adult patients, data on the paediatric patient population are few, but suggest differences in aetiology and host factors between adults and paediatric patients. Besides immunosuppressive states induced by intensive chemotherapy in paediatric malignancies, children with inherited defects in host defence, such as chronic granulomatous disease represent unique patient populations susceptible for IA. |
| Technique | PCR |
| Analysis Method | Aspergillus-specific PCR |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | Aspergillus-specific PCR |
| Up Regulation Down Regulation | Positive |
| Sequence Data | GenBank Accession ID: AB008401 |
| External Link | None |
