| DB ID | MyCo_5472 |
| Title | Evaluation of real-time PCR, galactomannan enzyme-linked immunosorbent assay (ELISA), and a novel lateral-flow device for diagnosis of invasive aspergillosis |
| Year | 2013 |
| PMID | 23486712 |
| Fungal Diseases involved | Invasive aspergillosis |
| Associated Medical Condition | None |
| Genus | Aspergillus |
| Species | spp. |
| Organism | Aspergillus spp. |
| Ethical Statement | The study was conducted as a retrospective performance assessment not requiring ethical approval. |
| Site of Infection | None |
| Opportunistic invasive | Opportunistic |
| Sample type | Body fluid |
| Sample source | EDTA Blood |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | 28S rRNA gene |
| Biomarker Full Name | 28S rRNA gene |
| Biomarker Type | Diagnostic |
| Biomolecule | Gene |
| Geographical Location | UK |
| Cohort | The study comprised 103 adult hematology patients at high risk of developing IA, including eight proven cases of IA, 14 probable cases, 22 possible cases, and 59 controls not achieving an EORTC/MSG diagnosis. |
| Cohort No. | 103 |
| Age Group | None |
| P Value | p=0.0001 |
| Sensitivity | 0.818 |
| Specificity | 0.864 |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Invasive aspergillosis (IA) is a serious infection in immunocompromised patients. Delays in diagnosis contribute to the high morbidity and mortality associated with this disease. The detection of circulating biomarkers (DNA or antigens) indicative of IA is an attractive strategy that allows frequent testing, increasing the opportunity for detecting early infection before overt disease develops. High assay sensitivity and negative predictive value (NPV) can exclude disease, preventing unnecessary treatment in patients who are consistently negative. The number of biomarker test options is limited. |
| Technique | PCR |
| Analysis Method | Aspergillus RT-PCR |
| ELISA kits | Platelia ELISA kits (Bio-Rad, United Kingdom) |
| Assay Data | None |
| Validation Techniques used | ELISA, PCR, LFD |
| Up Regulation Down Regulation | Increase |
| Sequence Data | None |
| External Link | None |
