| DB ID | MyCo_5457 |
| Title | Combining Aspergillus mitochondrial and ribosomal QPCR, in addition to galactomannan assay, for early diagnosis of invasive aspergillosis in hematology patients |
| Year | 2015 |
| PMID | 26162471 |
| Fungal Diseases involved | Invasive aspergillosis |
| Associated Medical Condition | Haematology patients |
| Genus | Aspergillus |
| Species | fumigatus |
| Organism | Aspergillus fumigatus |
| Ethical Statement | None |
| Site of Infection | None |
| Opportunistic invasive | Invasive |
| Sample type | Body fluid |
| Sample source | Serum |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | ITS |
| Biomarker Full Name | Internal Transcribed Spacer |
| Biomarker Type | Diagnostic |
| Biomolecule | Gene |
| Geographical Location | France |
| Cohort | The study was performed in the Hematology ICU at Besanc ¸on University Hospital from March 2012 to December 2013 (21 months); 185 patients out of 480 admissions were included in the study. |
| Cohort No. | 185 |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Invasive aspergillosis (IA) remains a clinically challenging opportunistic fungal infection and leads to significant mor-bidity and mortality in heavily immunocompromised pa- tients. The early diagnosis of IA and the prompt initiation of antifungal therapy have been shown to reduce mortality in patients with IA. Diagnosis of IA is rarely proven by histological examination and is frequently a probable or possible diagnosis based on a combination of clinical, ra- diological with and without microbiological criteria. Aspergillus galactomannan (GM) antigen is the biomarker most often used in current practice to screen for aspergillosis in leukemia patients. |
| Technique | PCR |
| Analysis Method | AfQPCR |
| ELISA kits | PlateliaTM Aspergillus EIA assay (Bio-Rad, Munich, Germany) |
| Assay Data | None |
| Validation Techniques used | GM-Platelia™ Aspergillus Ag ELISA, AfQPCR |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
| External Link | None |
