| DB ID | MyCo_5287 |
| Title | Influence of Aspergillus fumigatus conidia viability on murine pulmonary microRNA and mRNA expression following subchronic inhalation exposure |
| Year | 2016 |
| PMID | 27473664 |
| Fungal Diseases involved | Aspergillosis |
| Associated Medical Condition | None |
| Genus | Aspergillus |
| Species | fumigatus |
| Organism | Aspergillus fumigatus |
| Ethical Statement | All animal procedures were performed under a NIOSH Animal Care and Use Committee approved protocol. |
| Site of Infection | Lungs |
| Opportunistic invasive | None |
| Sample type | Biopsy |
| Sample source | Extracted RNA |
| Host Group | Animal |
| Host Common name | Mice |
| Host Scientific name | Mus musculus |
| Biomarker Name | miR-30c-5p |
| Biomarker Full Name | miR-30c-5p |
| Biomarker Type | Diagnostic |
| Biomolecule | miRNA |
| Geographical Location | Morgantown, WV |
| Cohort | Female B6C3F1/N mice, aged 5ā6 weeks old were acquired from the National Toxicology Program and mouse colony housed at Taconic (Germantown, NY). Mice were acclimated for approximately one week prior to exposures and housed in filtered, ventilated polycarbonate cages in groups of 5 on autoclaved hardwood chip bedding. |
| Cohort No. | None |
| Age Group | None |
| P Value | pā¤0.05 |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | -21.05 |
| Pathway | None |
| Disease Introduction Mechanism | Exposure to fungal bioaerosols in indoor environments has been associated with adverse respiratory health effects. Interest in understanding the mechanisms associated with fungal exposures has increased following recent natural disasters, including hurricanes Katrina, Rita, and more recently Sandy, that have resulted in the prolific contamination of flooded indoor building materials. Aspergillus fumigatus is a saprophytic mold naturally found in the environment that produces respirable asexual conidia. In some damp indoor environments, A. fumigatus can be found growing on common building materials such as wood, gypsum board, and chipboard and disturbance of these contaminated building materials can result in elevated personal exposure. To date, the influence of conidia viability on the microRNA (miRNA) and messenger RNA (mRNA) profiles underlying pulmonary immune and toxicological responses to A. fumigatus exposure are not fully characterized. |
| Technique | Analytic |
| Analysis Method | MicroRNA Array Profiling |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | MicroRNA Array Profiling, qRT-PCR |
| Up Regulation Down Regulation | Down regulated |
| Sequence Data | None |
| External Link | None |
