| DB ID | MyCo_5176 |
| Title | Comparative metabolomics implicates threitol as a fungal signal supporting colonization of Armillaria luteobubalina on eucalypt roots |
| Year | 2020 |
| PMID | 31797388 |
| Fungal Diseases involved | Armillaria root rot |
| Associated Medical Condition | None |
| Genus | Armillaria |
| Species | luteobuablina |
| Organism | Armillaria luteobuablina |
| Ethical Statement | None |
| Site of Infection | Root |
| Opportunistic invasive | None |
| Sample type | Extracted Seedlings |
| Sample source | Extracted Seedlings |
| Host Group | Plant |
| Host Common name | Eucalypt |
| Host Scientific name | Eucalyptus grandis |
| Biomarker Name | Threitol |
| Biomarker Full Name | Threitol |
| Biomarker Type | Diagnostic |
| Biomolecule | Metabolite |
| Geographical Location | Australia |
| Cohort | To establish the experiment, E. grandis seeds (seedlot 20974) were obtained from the Commonwealth Scientific and Industrial Research Organisation (Clyton, Victoria, Australia) tree seed centre. Seeds sterilization was performed by submerging the seeds in 30% (v/v) H2O2 for 10 min followed by washing in sterilized deionized water for 5 min five times. After sterilization, seeds were germinated in 1% water agar for 3 weeks before transferring into half‐strength Modified Melin‐Norkrans (MMN) medium. The E. grandis seedlings were cultivated in a controlled growth chamber (22–30°C; 16‐hr light cycle) for 1 month prior to the presymbiotic interaction with microbes. |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Armillaria root disease is a ubiquitous root disease threatening numerous tree species in the world including a wide range of eucalypt species. Armillaria‐infected eucalypts usually show symptoms such as white mycelical sheets under the bark, white rot of sapwood, black rhizomorphs penetrating root surfaces, and honey‐ coloured mushrooms clusters. Further aboveground symptoms include reduced growth of the host, distress cone crop, and crown thinning. As Armillaria usually causes only a minor disturbance to native eucalypts forests and plantations, these fungi are often considered as an unimportant, indigenous soil‐borne pathogen of eucalypts in Australia. However, when trees are under stress due to drought or temperature extremes, they became more prone to Armillaria disease. Therefore, in view of changing global climatic conditions, root rot attributed to Armillaria infection could become more prominent in the future. |
| Technique | Liquid chromatography |
| Analysis Method | Metabolomics Approach |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | Metabolomics Approach, RT-qPCR |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
| External Link | None |
