| DB ID | MyCo_5131 |
| Title | Metabolome Dynamics of Smutted Sugarcane Reveals Mechanisms Involved in Disease Progression and Whip Emission |
| Year | 2017 |
| PMID | 28620397 |
| Fungal Diseases involved | Sugarcane smut disease |
| Associated Medical Condition | None |
| Genus | Sporisorium |
| Species | scitamineum |
| Organism | Sporisorium scitamineum |
| Ethical Statement | Sporisorium scitamineum SSC39 teliospores were collected as described by Taniguti et al. (2015). Experiments were performed using the smut susceptible Brazilian commercial variety of sugarcane, “RB925345”. The healthy plants used to conduct the experiments were collected from the experimental station of the Genetics Department at ESALQ-University of São Paulo, Piracicaba, São Paulo, Brazil. No special permits were necessary for the teliospores or cane collection to scientific research. |
| Site of Infection | None |
| Opportunistic invasive | None |
| Sample type | Plant extracts |
| Sample source | Plant extracts |
| Host Group | Plant |
| Host Common name | Sugarcane |
| Host Scientific name | Saccharum |
| Biomarker Name | Acidissiminol epoxide |
| Biomarker Full Name | Acidissiminol epoxide |
| Biomarker Type | Diagnostic |
| Biomolecule | Metabolite |
| Geographical Location | Brazil |
| Cohort | Sporisorium scitamineum SSC39 teliospores with viability 95% were used to inoculate single budded setts of 7-month-old plants. Setts were subjected to disinfection following three steps: thermal treatment (30 min in water bath at 52◦C; 1 kg of setts per 6 L of water); 10 min in sodium hypochlorite solution (4 mL.L−1) and three washes in distilled water. Setts were than inoculated using the puncture method (106 teliospores.mL−1 in saline solution; NaCl2 0.85 M). Mock-inoculated plants were prepared only with saline solution (control plants). Inoculated and control plants were placed in greenhouse benches in a completely randomized experimental design. The experiment was conducted from February to May of 2015. Plants were irrigated during early morning hours every day and no temperature control was used. Sampling was done from buds 5 days after inoculation (DAI), and the meristematic region of the main culm at 65 DAI, 100 DAI, and 120 DAI. The last corresponded to the time immediately after whip emission. Each time point analyzed was represented by five biological replicates composed of pools of three plants for 5, 65, and 100 DAI samples. The 120 DAI replicates were represented by one plant (Supporting Information Figure S1). All samples were frozen in liquid nitrogen immediately after collection and stored at−80◦C. Infected plants were compared to control samples of the same age. |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | None |
| Technique | Gas chromatography |
| Analysis Method | Metabolomics Approach |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | Gas chromatography coupled with mass spectrometry (GC-TOF-MS) and liquid chromatography coupled to electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS), qRT-PCR |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
| External Link | None |
