| DB ID | MyCo_4860 |
| Title | Metabolite profiling of susceptible and resistant wheat (Triticum aestivum) cultivars responding to Puccinia striiformis f. sp. tritici infection |
| Year | 2023 |
| PMID | 37264330 |
| Fungal Diseases involved | Puccinia striiformis f. sp. tritici infection |
| Associated Medical Condition | None |
| Genus | Puccinia |
| Species | striiformis f. sp. tritici |
| Organism | Puccinia striiformis f. spp. tritici |
| Ethical Statement | The authors confirm that all the experimental research done on cultivated plants and carried out in accordance with relevant institutional, national, and international guidelines, standards, and legislation. The plants used were cultivated under controlled conditions and thus posed no threat to other plant species, and no specific permission was required to collect the required sample material used in this study. The plants were cultivated with seeds obtained from the Small Grain Centre, Agricultural Research Council (ARC-SGI), South Africa and sample material was collected and stored at the University of Johannesburg, Research Centre for Plant Metabolomics until utilisation. |
| Site of Infection | None |
| Opportunistic invasive | None |
| Sample type | Biopsy |
| Sample source | Tissue Supernatant |
| Host Group | Plant |
| Host Common name | Wheat |
| Host Scientific name | Triticum aestivum |
| Biomarker Name | Luteolin-6-C-hexoside-O-hexoside |
| Biomarker Full Name | Luteolin-6-C-hexoside-O-hexoside |
| Biomarker Type | Diagnostic |
| Biomolecule | Metabolite |
| Geographical Location | South Africa |
| Cohort | Wheat cultivars were selected to elucidate the responsive mechanisms of the susceptible variety (Gariep) to Pst compared to its resistant counterpart (Koonap) at a metabolic level. Previous studies have reported metabolic perturbations in plants due to plant-pathogen interactions. In the current study, methanolic extracts of Pst-treated and untreated Koonap and Gariep wheat cultivars were analysed on an LC-MS system to acquire chromatographic and MS profiles of the samples. |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Puccinia striiformis f. sp. tritici (Pst) is an economically devasting disease that is prominent in cereal crops such as wheat (Triticum aestivum). The fungal pathogen can cause approximately 30–70% losses in crop productivity and yields. Pst has become difficult to manage due to its ease of transmission through wind dispersal over long distances, and intercontinental dispersal has been previously reported. The ease of transmission has resulted in further destruction because of new and more virulent strains infecting crops previously resistant to a different strain. |
| Technique | Liquid chromatography |
| Analysis Method | Liquid Chromatography-Mass Spectrometry (LC–MS) based metabolomics |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | Liquid Chromatography-Mass Spectrometry (LC–MS) based metabolomics |
| Up Regulation Down Regulation | Down regulated |
| Sequence Data | None |
| External Link | None |
