| DB ID | MyCo_4821 |
| Title | A Metabolomic Study of Epichloë Endophytes for Screening Antifungal Metabolites |
| Year | 2022 |
| PMID | 35050159 |
| Fungal Diseases involved | Epichloë endophytes |
| Associated Medical Condition | None |
| Genus | Epichloë |
| Species | spp. |
| Organism | Epichloë spp. |
| Ethical Statement | None |
| Site of Infection | None |
| Opportunistic invasive | None |
| Sample type | Plant extracts |
| Sample source | Extracted DNA |
| Host Group | Plant |
| Host Common name | Perennial ryegrass |
| Host Scientific name | Lolium perenne |
| Biomarker Name | M3 |
| Biomarker Full Name | M3 |
| Biomarker Type | Diagnostic |
| Biomolecule | Metabolite |
| Geographical Location | Australia |
| Cohort | All plant material was obtained from a glasshouse maintained (natural day lengths and a mean temperature of 22◦C) collection at the Agriculture Victoria, Bundoora, Victoria, Australia. Prior to harvesting for metabolomics analysis, the presence and identity of endophyte strains was confirmed by the extraction of DNA using Qiagen MagAttract DNA (Qiagen, Hilden, Germany) and SNP-based diagnostic testing, KASP analysis (Kompetitive Allele Specific PCR) (KASP—, LGC Genomics, Teddington, UK). |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Perennial ryegrass and tall fescue, widely utilized for pasture and turf, are the key source of feed for grazing animals across agricultural industries globally. Pasture yield, quality, and performance directly affect animal health performance, product quality, and industry profitability. Therefore, pasture species and varieties are extensively studied for their yield properties and performance traits to identify the best performing pasture grasses suitable for a vast range of climatic regions throughout the world. Yield, growth parameters, digestibility, nutrient concentrations, abiotic and biotic stress tolerance are some of the characteristics widely studied. For decades genetic research has been carried out to develop pasture cultivars that are better performing in target climates. Epichloë sp., of the family Clavicipitaceae, are fungal symbionts of cool-season grasses from the Poaceae sub-family Pooideae. Epichloë sp. endophytes improve host grass performance by providing the benefits of plant growth promotion, abiotic stress tolerance, pest tolerance, and disease resistance to the host plant by the production of functional secondary metabolites. Due to the importance of these alkaloidal metabolites in animal welfare and pest protection, comprehensive investigations of their biological activity, mode of action, and quantitative levels in grasses infected with Epichloë sp. endophytes have been reported in the literature. |
| Technique | Liquid chromatography |
| Analysis Method | Liquid Chromatography-Mass Spectrometry (LC–MS) based metabolomics |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | Liquid Chromatography-Mass Spectrometry (LC–MS) based metabolomics |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
| External Link | None |
