| DB ID | MyCo_4204 |
| Title | Immunoproteomic Analysis Reveals Novel Candidate Antigens for the Diagnosis of Paracoccidioidomycosis Due to Paracoccidioides lutzii |
| Year | 2020 |
| PMID | 33322269 |
| Fungal Diseases involved | Paracoccidioidomycosis |
| Associated Medical Condition | None |
| Genus | Paracoccidioides |
| Species | lutzii |
| Organism | Paracoccidioides lutzii |
| Ethical Statement | This study was approved (CAAE: 17177613.6.0000.5541) by the research ethics committee of Federal University of Mato Grosso, and protocol numbers 1796–10 and CEP 3147220120 were approve by the counterpart committee of Federal University of São Paulo. All adult subjects provided informed written consent, and the study was also approved under number 288.250/CEP/HUJM/UFMT. |
| Site of Infection | None |
| Opportunistic invasive | None |
| Sample type | Body fluid |
| Sample source | Serum |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | NAD(P)H: quinone oxidoreductase, type IV |
| Biomarker Full Name | NAD(P)H: quinone oxidoreductase, type IV |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | Brazil |
| Cohort | The Paracoccidioides spp. strains used in this study were the classical EPM208 and Pb18 (EPM16) isolates. A total of 20 sera from patients with confirmed PCM and 10 self-reported healthy normal donors were used in this investigation. Patients had the chronic form of PCM and exhibited clinical and laboratory signs of the disease, with pulmonary system involvement and mucosal or mucocutaneous lesions. PCM was confirmed in all patients by laboratory demonstration of pathognomonic “ship’s wheel” budding yeast cells and fungal isolation in vitro. |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Paracoccidioidomycosis (PCM) is a life-threatening systemic infection caused by the fungal pathogen Paracoccidioides brasiliensis and related species. The disease was first described in Brazil of the Americas. The genus Paracoccidioides was described in 1930 by Floriano de Almeida, and currently it infects at least 10 million people, being the most prevalent systemic mycosis in Latin America. The incidence of PCM is estimated to be one to three cases per 100,000 inhabitants, and the majority of cases occur in endemic areas of Brazil, Colombia, and Venezuela. The characteristics of the work environment can play a role, together with other risk factors, in PCM development. Therefore, PCM has been mostly reported in male rural workers during the most productive years of their lives, which poses a significant public health problem and causes substantial economic losses. Mortality associated with PCM varies between 6.1% and 7.6%, ranking it as the eighth most common cause of death among infectious and parasitic chronic diseases. |
| Technique | Bioinformatics analysis |
| Analysis Method | Immunoproteomic |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | Immunoproteomic, 2D Gel Electrophoresis |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
| External Link | None |
